Difference between revisions of "Team:SSTi-SZGD"

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<div class="col-xs-12 col-sm-10 name hidden-xs">Team:SSTi-SZGD</div>
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<div class="col-xs-12 col-sm-10 name visible-xs">SSTi-SZGD</div>
 
 
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<section class="col-xs-12 col-sm-10 ProjectVideo">
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<video width="100%" controls="controls">
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<source src="https://static.igem.org/mediawiki/2018/9/90/T--SSTi-SZGD--ProjectVideo.mp4" type="video/mp4" />
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<section id="description" class="container description">
 
 
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<div class="Hyaluronic">
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Hyaluronic acid micro factory : A bacterium produces low molecular weight hyaluronic acid
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<div class="col-xs-12 paragraph one">
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<!--{cn}透明质酸微工厂：一菌直接生产小分子透明质酸-->
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<div class="col-xs-12 col-md-6 content">
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In our modern world, millions of people do not hesitate to spend a fortune to achieve younger looking and vibrant appearances, thus the cosmetic industry keeps growing.
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<!--{cn}在我们的现代社会，数以百万计的人会不假思索地花一大笔钱，以获得更年轻和更加充满活力的外观，因此化妆品行业不断增长。-->
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Engineering strain : Bacillus subtilis
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<img src="https://static.igem.org/mediawiki/2018/thumb/4/41/T--SSTi-SZGD--home_break.png/187px-T--SSTi-SZGD--home_break.png"/>
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<!--{cn}工程菌株：枯草芽孢杆菌-->
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Genetic origin : Streptococcus equi and Leech
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<!--{cn}遗传起源：马链球菌和水蛭-->
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Hyaluronic acid is one of the mostly used ingredients in cosmetic products, thanks to its exceptional water retention ability that helps rejuvenating skin and reducing wrinkles.
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<!--{cn}透明质酸是化妆品中最常用的成分之一，因为它具有超强的保水能力，可以帮助皮肤恢复活力，减少皱纹。-->
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<img src="https://static.igem.org/mediawiki/2018/thumb/4/41/T--SSTi-SZGD--home_break.png/187px-T--SSTi-SZGD--home_break.png"/>
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read more
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Hyaulornic acid production via microbial fermentation is generally through
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<!--{cn}通过微生物发酵产生的下透明质酸通常是通过-->
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<i>Streptococus<!--{cn}兽疫链球菌--></i>
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species, which contains endotoxins. We constructed recombinant
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<!--{cn}，但其中含有内毒素。为了安全的大量生产透明质酸，我们构建了食品级安全菌-->
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<i>Bacillus subtilis<!--{cn}枯草芽孢杆菌--></i>
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, the food-grade safe species, to mass produce hyaluronic acid.
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<!--{cn}。-->
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<img src="https://static.igem.org/mediawiki/2018/thumb/4/41/T--SSTi-SZGD--home_break.png/187px-T--SSTi-SZGD--home_break.png"/>
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After covalently cross-link, low molecular weight hyaluronic acid (LMW-HA) forms stable hydrogel that is important for film-forming process. By introducing a hydrolase gene, we constructed a
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<!--{cn}低分子量透明质酸(LMW-HA)共价交联后形成稳定的水凝胶，对成膜过程具有重要意义。通过引入水解酶基因，构建了一株能够生物合成LMW-HA的-->
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<i>B. Subtilis<!--{cn}枯草芽孢杆菌--></i>
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strain that is able to biosynthesize LMW-HA.
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<!--{cn}菌株。-->
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<img src="https://static.igem.org/mediawiki/2018/thumb/4/41/T--SSTi-SZGD--home_break.png/187px-T--SSTi-SZGD--home_break.png"/>
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<img src="https://static.igem.org/mediawiki/2018/thumb/4/40/T--SSTi-SZGD--home_hamp.png/800px-T--SSTi-SZGD--home_hamp.png"/>
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We developed a novel cosmetic product: HA Micro-Needle Patch !!!
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<!--{cn}我们开发了一种新型的化妆品:透明质酸微针贴片!!!-->
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<a href="https://2018.igem.org/Team:SSTi-SZGD/Description"><img src="https://static.igem.org/mediawiki/2018/4/4f/T--SSTi-SZGD--home_Project.png"/></a>
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<a href="https://2018.igem.org/Team:SSTi-SZGD/Applied_Design"><img src="https://static.igem.org/mediawiki/2018/b/b9/T--SSTi-SZGD--home_Applied_Design.png"/></a>
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Abstract
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The production of hyaluronic acid has been changed from traditional animal tissue formulation to microbial fermentation. However, there is no report that tissue cells or microorganisms can di-rectly produce low molecular weight hyaluronic acid. In order to prepare low molecular weight hyaluronic acid, physical and chemical methods are needed. However, there are many drawbacks in physical and chemical methods, such as poor product stability, low efficiency, complex reac-tion conditions and possible environmental pollution.
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<!--{cn}透明质酸的生产已经从传统的动物组织配方转变为微生物发酵，但目前还没有关于组织细胞或微生物可以直接生产低分子透明质酸的报道，为了制备低分子透明质酸，需要采用物理和化学的方法，但在物化方法上存在着产品稳定性差、效率低、反应条件复杂、可能对环境造成污染等问题。-->
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This year our project constructed a recombinant strain Bacillus subtilis 168E which could directly produce different molecular weight hyaluronic acid products by regulating the activities of LHAase. Bacillus subtilis 168 was selected as the best chassis. The HasA gene and identified pre-cursor  genes (tuaD , gtaB , gluM , glmM and glmS) was transferred into Bacillus subtilis. Since hyaluronic acid of high molecular weight was produced at this time, we transferred the LHAase gene into Bacillus subtilis 168 which is from leech resources coding hyaluronidase. Therefore the hyaluronic acid could be enzymatic hydrolyzed to different molecular weight.
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<!--{cn}因此，我们打算构建一株能直接产生低分子透明质酸的菌株，发现枯草芽孢杆菌168为最佳选择，将hsA基因转入枯草芽孢杆菌168，并对透明质酸途径基因进行优化，使其大量产生透明质酸。由于此时产生了高分子量的透明质酸，我们将该基因转入枯草杆菌。最后，我们获得了一株能直接生产低分子透明质酸产品的重组枯草芽孢杆菌168 E。-->
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Our intended application is use low molecular weight hyaluronic acid to prepare microneedles and that hyaluronic acid can be dissolved and stuck in the skin under the action of body fluid when it is punctured into the human skin, microneedles allow the penetration of low molecular weight HA through to the dermal papillary cutis, which could not only play a cosmetic role. Be-cause hyaluronic acid is an endogenous substance of the human body and does not cause the in-flammatory reaction, therefore it is a new type of microneedle material and has a potential mar-ket.
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<!--{cn}通过查阅文献，我们认为低分子量的透明质酸可以作为制备微针的基质，当透明质酸被刺入人体皮肤时，透明质酸可以在体液的作用下溶解和附着在皮肤中，这不仅可以起到美容作用，因为透明质酸是人体内的一种内源性物质，而不是引起人体内源性物质的原因。炎症反应，是一种新型的微针材料，具有潜在的市场前景。-->
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Copyright © 2018 SSTi-SZGD All Rights Reserved.
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Latest revision as of 05:50, 2 November 2018

SSTi-SZGD
In our modern world, millions of people do not hesitate to spend a fortune to achieve younger looking and vibrant appearances, thus the cosmetic industry keeps growing.
Hyaluronic acid is one of the mostly used ingredients in cosmetic products, thanks to its exceptional water retention ability that helps rejuvenating skin and reducing wrinkles.
Hyaulornic acid production via microbial fermentation is generally through Streptococus species, which contains endotoxins. We constructed recombinant Bacillus subtilis , the food-grade safe species, to mass produce hyaluronic acid.
After covalently cross-link, low molecular weight hyaluronic acid (LMW-HA) forms stable hydrogel that is important for film-forming process. By introducing a hydrolase gene, we constructed a B. Subtilis strain that is able to biosynthesize LMW-HA.
We developed a novel cosmetic product: HA Micro-Needle Patch !!!
Copyright © 2018 SSTi-SZGD All Rights Reserved.

Copyright © 2018 SSTi-SZGD All Rights Reserved.