Difference between revisions of "Team:Paris Bettencourt/Results"

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<h1>Key Results</h1>
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h2>Key results from the Human Practices</h2>
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<h3>What should this page contain?</h3>
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<li> Clearly and objectively describe the results of your work.</li>
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<li>By talking to experts, farmers and veterinaries, we identified that antimicrobial resistance of coliforms affecting piglets is a major concern</li>
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<li> Future plans for the project. </li>
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<li>We obtained access to raw data from ANSES and found out that this data highlights amoxicillin and tetracycline as drugs with the most frequent evolved resistance</li>
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<li> Considerations for replicating the experiments. </li>
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<h2>Key results from the STAR CORE Design and Production</h2>
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<li>We successfully cloned 131 STAR CORE combinations.</li>
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<li>9 out of the 11 proteins we expressed exhibited antibacterial activity comparable to existing antibiotics and AMPs.</li>
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<h2>Key results from the Testing</h2>
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<h3>Describe what your results mean </h3>
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<li>The STAR CORE we have obtained show antimicrobial activity.</li>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li>8 out of the 10 constructs showed antimicrobial activity towards E. coli while 9 out of the 10 constructs displayed antimicrobial potential towards B. subtilis.</li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li>The top performing STAR CORE was the Ferritin-Alyteserin fusion, with an activity almost 10 times higher than that of the control.
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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Some StarCores exhibited species-specific killing, being particularly active against E. coli.</li>
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<li>StarCores of varying geometry displayed similar MIC values.</li>
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<li>There was no clear correlation between the geometry of the STAR CORE and their antimicrobial activity, indicating a non-specific mechanism of action.</li>
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<h2>Key results from Optimization and Modeling</h2>
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<h3> Project Achievements </h3>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<li>Decreasing net charge on the peptide improves expression efficiency, however it decreases its antibacterial activity</li>
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<h3>Inspiration</h3>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Latest revision as of 12:19, 19 November 2018

Key Results

Key results from the Human Practices

  • By talking to experts, farmers and veterinaries, we identified that antimicrobial resistance of coliforms affecting piglets is a major concern
  • We obtained access to raw data from ANSES and found out that this data highlights amoxicillin and tetracycline as drugs with the most frequent evolved resistance

    • Key results from the STAR CORE Design and Production

  • We successfully cloned 131 STAR CORE combinations.
  • 9 out of the 11 proteins we expressed exhibited antibacterial activity comparable to existing antibiotics and AMPs.

    • Key results from the Testing

  • The STAR CORE we have obtained show antimicrobial activity.
  • 8 out of the 10 constructs showed antimicrobial activity towards E. coli while 9 out of the 10 constructs displayed antimicrobial potential towards B. subtilis.
  • The top performing STAR CORE was the Ferritin-Alyteserin fusion, with an activity almost 10 times higher than that of the control. Some StarCores exhibited species-specific killing, being particularly active against E. coli.
  • StarCores of varying geometry displayed similar MIC values.
  • There was no clear correlation between the geometry of the STAR CORE and their antimicrobial activity, indicating a non-specific mechanism of action.

    • Key results from Optimization and Modeling

  • Decreasing net charge on the peptide improves expression efficiency, however it decreases its antibacterial activity
    • Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    paris-bettencourt-2018@cri-paris.org