Difference between revisions of "Team:Paris Bettencourt/Active Testing"

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<li>Unbalanced amino acid usage, with a high density of lysine and arginine residues.</li>
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<li><b>Antimicrobial activity:</b> Do they kill bacteria? What is their effective concentration?</li>
 
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     <li>High density of positive charges, leading to misfolding and aggregation.</li>
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     <li><b>Specificity:</b> Do they selectively kill certain types of bacteria? </li>
 
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     <li>Direct toxicity to cell components caused by AMP activity.</li>
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     <li><b>Mechanism of action:</b> How do they kill the bacteria?</li>
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    <li><b>Toxicity:</b> How toxic are they to human cells? </li>
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    <li><b>Geometry:</b> Does the structure of a StarCore change the function?</li>
 
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Revision as of 22:50, 5 December 2018


Active Testing

Following the production phase of our project, we had 10 StarCore constructs to test. We set out to characterize their antimicrobial properties and mechanism of action. We had many questions in the following categories.

  • Antimicrobial activity: Do they kill bacteria? What is their effective concentration?

  • Specificity: Do they selectively kill certain types of bacteria?

  • Mechanism of action: How do they kill the bacteria?

  • Toxicity: How toxic are they to human cells?

  • Geometry: Does the structure of a StarCore change the function?

    • Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    paris-bettencourt-2018@cri-paris.org