Difference between revisions of "Team:DTU-Denmark"

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<h1> Building Mycotextures </h1>
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<p>The DTU biobuilders are looking forward to enter iGEM once again! This year, we are working to develop a toolbox so properties of fungi can be manipulated and exploited to build fungal materials.</p>
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<img id="frontpagebigpic" src="https://static.igem.org/mediawiki/2018/9/90/T--DTU-Denmark--frontpagepicture.png" style="max-width: 100%;" >
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<div id="frontfirstwrapperid" class="frontfirstwrapper">
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<div id="frontresumething" class="interlabspace frontresume" >
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<div class="spacefront">
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h1 style="font-size: 9vw;padding-bottom:20px;" id="frontheadline1">Fungal building materials for extreme environments</h1>
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</ul>
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<h5 style="color:#fff;text-align: center;">Colonization of uninhabitable areas, like Mars, will require building materials to be transported to the site of deployment. Transport limitations such as space and weight make this process very expensive. Based on these challenges, we propose to make building materials from fungal mycelium to be grown on site. Our project is focused on how to optimize the material properties of the fungi through engineering of basic fungal characteristics. Our initial studies identified <i>Aspergillus oryzae</i> as the best candidate chassis for material properties and ease of genetic engineering. Based on our choice of fungus, we decided to increase the gene expression of melanin to improve <i>A. oryzae’s</i> capabilities of withstanding UV radiation and change the colors of the fungi by inserting a blue chromoprotein gene. Furthermore, we have designed a final geometric structure that can withstand external conditions and reduce the amount of work needed to assemble it.
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</h5>
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<button type="button" class="btn btn-default" onclick="window.location.href='https://2018.igem.org/Team:DTU-Denmark/jamboree'" formtarget="_blank">Presentation and Poster</button>
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</div>
 
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<div class="frontspace frontfirstwrapper2">
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<h1 id="fronttheplan">The Plan</h1>
  
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<h2 class="front_phase_headline">PHASE ONE - Preparations on Earth</h2>
  
  
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<div class="column full_size" >
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</div>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<div class="container-fluid">
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<div class="col-xs-2 frontcomputer">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#protectionfromuv"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/b/b4/T--DTU-Denmark--front-icon-11.png"></a>
 
</div>
 
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<h5 class="media heading">We would begin by genetically optimizing a fungal species for the purpose of making construction materials.</h5>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#protectionfromuv"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/b/b4/T--DTU-Denmark--front-icon-11.png"></a>
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<div class="clear extra_space"></div>
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<h5  class="media heading">Vigorous testing and verification on the fungus would be performed on earth to ensure proper gene function and behavior before launch.</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Results"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/1/1d/T--DTU-Denmark--front-icon-12.png"></a>
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</div>
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<div class="frontleft">
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<div class="col-xs-2 frontcomputer">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/d/d1/T--DTU-Denmark--front-icon-13.png"></a>
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<div class="col-xs-10">
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<h5 class="media heading">Various simulations and models will play a crucial part in designing sustainable structures that can protect humans against the hostile Martian environment.</h5>
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</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/d/d1/T--DTU-Denmark--front-icon-13.png"></a>
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</div>
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</div>
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</div>
  
  
  
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<div class="column third_size" >
 
  
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<h3> Uploading pictures and files </h3>
 
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 
  
  
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="button_link">
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<div class="frontheadlines">
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<a href="https://2018.igem.org/Special:Upload">
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<h2 class="front_phase_headline">PHASE TWO - Inventory</h2>
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UPLOAD FILES
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</a>
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</div>
 
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<div class="frontspace frontphases">
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<div class="frontleft frontcomputer">
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#missionarchitecture"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/9/9f/T--DTU-Denmark--front-icon-21.png"></a>
 
</div>
 
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<div class="col-xs-10">
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<h3> Wiki template information </h3>
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<h5 class="media heading">The space shuttle would need to have the essentials for our fungus to be grown.
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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</h5>
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</div>  
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<div class="col-xs-2 frontmobile" >
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#missionarchitecture"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/9/9f/T--DTU-Denmark--front-icon-21.png"></a>
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</div>
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<div class="frontright">
  
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<div class="col-xs-10">
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<h5  class="media heading">Vials of spores from our GMO fungus would be prepared and a necessary means of  biomass, most likely cyanobacteria, will be included.
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</h5>
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<div class="col-xs-2">
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<div class="highlight decoration_B_full">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#growingfungusonMars"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/5/53/T--DTU-Denmark--front-icon-22.png"></a>
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<h3> Editing your wiki </h3>
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</div>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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</div>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2018.igem.org/wiki/index.php?title=Team:DTU-Denmark&action=edit">  
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<div class="frontleft">
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EDIT PAGE
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<div class="col-xs-2 frontcomputer">
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</a>
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#buildstructures"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/7/72/T--DTU-Denmark--front-icon-23.png"></a>
 
</div>
 
</div>
  
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<div class="col-xs-10">
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<h5 class="media heading">Specific molds will let the fungus grow in a desired shape. Creating the pieces for the dome.
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</h5>
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</div>
  
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#buildstructures"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/7/72/T--DTU-Denmark--front-icon-23.png"></a>
 
</div>
 
</div>
 
</div>
 
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<div class="frontright">
  
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<div class="col-xs-10">
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<h5  class="media heading">General laboratory tools will have to be included to perform the needed work.</h5>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Safety"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/1/10/T--DTU-Denmark--front-icon-24.png"></a>
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<div class="frontheadlines">
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<h2 class="front_phase_headline">PHASE THREE - Producing the Sheets</h2>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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<div class="frontspace frontphases">
  
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<div class="frontleft">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<div class="col-xs-2 frontcomputer">
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<ul>
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2b/T--DTU-Denmark--front-icon-31.png"></a>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
 
</div>
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<div class="col-xs-10">
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<h5 class="media heading">Plate production will be established inside a provisional inflatable tent.
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</h5>
 
</div>
 
</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2b/T--DTU-Denmark--front-icon-31.png"></a>
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</div>
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<div class="frontright">
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<div class="col-xs-10">
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<h5  class="media heading">Biomass and spores will be combined in easy-to-manage molds.
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</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/f/ff/T--DTU-Denmark--front-icon-32.png"></a>
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</div>
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</div>
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<div class="frontleft">
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<div class="col-xs-2 frontcomputer">
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<a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/9/96/T--DTU-Denmark--front-icon-33.png"></a>
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</div>
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<div class="col-xs-10">
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<h5 class="media heading">Growth in a confined space will let the fungus conform into the desired shape.
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</h5>
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</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/9/96/T--DTU-Denmark--front-icon-33.png"></a>
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<div class="frontright">
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<h5  class="media heading">When the mold is appropriately filled, the fungus will be extracted and killed as preparation for the building process.</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Results-choosing-organism"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/d/db/T--DTU-Denmark--front-icon-34.png"></a>
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</div>
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<div class="frontheadlines">
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<h2 class="front_phase_headline" >PHASE FOUR - Assembly</h2>
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<div class="frontspace frontphases">
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<div class="frontleft">
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<div class="col-xs-2 frontcomputer">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2e/T--DTU-Denmark--front-icon-41.png"></a>
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<h5 class="media heading">The produced materials are assembled to create larger habitat structures. Domes will likely be used as these are best at distributing the forces of internal pressure.
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</h5>
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</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2e/T--DTU-Denmark--front-icon-41.png"></a>
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</div>
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</div>
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<div class="frontright">
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<div class="col-xs-10">
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<h5  class="media heading">Given the high pressure difference between human habitats and the Martian atmosphere, the final structures will have to be sealed as to make the structure completely airtight.
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</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/d/df/T--DTU-Denmark--front-icon-42.png"></a>
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</div>
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</div>
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<div class="frontleft">
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<div class="col-xs-2 frontcomputer">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#melanin"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/a/a1/T--DTU-Denmark--front-icon-43.png"></a>
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</div>
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<div class="col-xs-10">
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<h5 class="media heading">At this point, otherwise optimized fungal species can be used to synthesize a range of useful biochemicals and materials.
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</h5>
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</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#melanin"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/a/a1/T--DTU-Denmark--front-icon-43.png"></a>
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</div>
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</div>
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<div class="frontright">
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<div class="col-xs-10">
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<h5  class="media heading">The cyanobacteria used in the production of fungal materials can also be used to supply oxygen to the habitat.</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://2018.igem.org/Team:DTU-Denmark/Design#growingfungusonMars"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/e/e5/T--DTU-Denmark--front-icon-44.png"></a>
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</div>
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</div>
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<div class="frontheadlines">
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<h2 class="front_phase_headline">Why do we want to land on Mars?</h2>
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<div class="frontspace frontphases">
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<div class="frontleft">
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<div class="col-xs-2 frontcomputer">
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<a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/8/8e/T--DTU-Denmark--front-icon-51.png"></a>
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</div>
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<div class="col-xs-10">
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<h5 class="media heading">The first space race that led to the moon landing was hugely influenced by political currents, but the will to expand is a notion that dates back to the period after the era of imperialism.
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</h5>
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</div>
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<div class="col-xs-2 frontmobile">
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<a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/8/8e/T--DTU-Denmark--front-icon-51.png"></a>
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</div>
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</div>
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<div class="frontright">
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<div class="col-xs-10">
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<h5  class="media heading">Many parallels from the moon landing can be drawn to the current race of getting the human race to Mars, where it can be concluded that getting there is inevitable.
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</h5>
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</div>
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<div class="col-xs-2">
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<a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/0/07/T--DTU-Denmark--front-icon-52.png"></a>
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</div>
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</div>
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<div class="frontspace frontresume" >
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<div class="spacefront">
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<h1 id="frontheadline2">Challenges</h1>
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Designing easily deployable production facilities that can protect the cultivation systems from the Martian environment.
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Latest revision as of 23:15, 6 December 2018

Fungal building materials for extreme environments

Colonization of uninhabitable areas, like Mars, will require building materials to be transported to the site of deployment. Transport limitations such as space and weight make this process very expensive. Based on these challenges, we propose to make building materials from fungal mycelium to be grown on site. Our project is focused on how to optimize the material properties of the fungi through engineering of basic fungal characteristics. Our initial studies identified Aspergillus oryzae as the best candidate chassis for material properties and ease of genetic engineering. Based on our choice of fungus, we decided to increase the gene expression of melanin to improve A. oryzae’s capabilities of withstanding UV radiation and change the colors of the fungi by inserting a blue chromoprotein gene. Furthermore, we have designed a final geometric structure that can withstand external conditions and reduce the amount of work needed to assemble it.

The Plan

PHASE ONE - Preparations on Earth

We would begin by genetically optimizing a fungal species for the purpose of making construction materials.
Vigorous testing and verification on the fungus would be performed on earth to ensure proper gene function and behavior before launch.
Various simulations and models will play a crucial part in designing sustainable structures that can protect humans against the hostile Martian environment.

PHASE TWO - Inventory

The space shuttle would need to have the essentials for our fungus to be grown.
Vials of spores from our GMO fungus would be prepared and a necessary means of biomass, most likely cyanobacteria, will be included.
Specific molds will let the fungus grow in a desired shape. Creating the pieces for the dome.
General laboratory tools will have to be included to perform the needed work.

PHASE THREE - Producing the Sheets

Plate production will be established inside a provisional inflatable tent.
Biomass and spores will be combined in easy-to-manage molds.
Growth in a confined space will let the fungus conform into the desired shape.
When the mold is appropriately filled, the fungus will be extracted and killed as preparation for the building process.

PHASE FOUR - Assembly

The produced materials are assembled to create larger habitat structures. Domes will likely be used as these are best at distributing the forces of internal pressure.
Given the high pressure difference between human habitats and the Martian atmosphere, the final structures will have to be sealed as to make the structure completely airtight.
At this point, otherwise optimized fungal species can be used to synthesize a range of useful biochemicals and materials.
The cyanobacteria used in the production of fungal materials can also be used to supply oxygen to the habitat.

Why do we want to land on Mars?

The first space race that led to the moon landing was hugely influenced by political currents, but the will to expand is a notion that dates back to the period after the era of imperialism.
Many parallels from the moon landing can be drawn to the current race of getting the human race to Mars, where it can be concluded that getting there is inevitable.

Challenges

Researching and optimizing a fungal species for the purpose of mycotecture.

Designing easily deployable production facilities that can protect the cultivation systems from the Martian environment.

We have not researched whether the structural properties depend on being on earth, eg. lower pressure compromising strength.



The team received a bronze medal for their project