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− | + | <div id="team_content" class="igem_2018_team_content"> | |
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+ | <img id="frontpagebigpic" src="https://static.igem.org/mediawiki/2018/9/90/T--DTU-Denmark--frontpagepicture.png" style="max-width: 100%;" > | ||
+ | </div> | ||
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+ | <style> | ||
+ | .frontlink:hover{opacity: 0.8; | ||
+ | filter: alpha(opacity=80);} | ||
+ | </style> | ||
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− | + | <div id="frontfirstwrapperid" class="frontfirstwrapper"> | |
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− | <div class=" | + | <div id="frontresumething" class="interlabspace frontresume" > |
− | < | + | <div class="spacefront"> |
− | < | + | <h1 style="font-size: 9vw;padding-bottom:20px;" id="frontheadline1">Fungal building materials for extreme environments</h1> |
− | + | <h5 style="color:#fff;text-align: center;">Colonization of uninhabitable areas, like Mars, will require building materials to be transported to the site of deployment. Transport limitations such as space and weight make this process very expensive. Based on these challenges, we propose to make building materials from fungal mycelium to be grown on site. Our project is focused on how to optimize the material properties of the fungi through engineering of basic fungal characteristics. Our initial studies identified <i>Aspergillus oryzae</i> as the best candidate chassis for material properties and ease of genetic engineering. Based on our choice of fungus, we decided to increase the gene expression of melanin to improve <i>A. oryzae’s</i> capabilities of withstanding UV radiation and change the colors of the fungi by inserting a blue chromoprotein gene. Furthermore, we have designed a final geometric structure that can withstand external conditions and reduce the amount of work needed to assemble it. | |
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+ | </h5> | ||
+ | <button type="button" class="btn btn-default" onclick="window.location.href='https://2018.igem.org/Team:DTU-Denmark/jamboree'" formtarget="_blank">Presentation and Poster</button> | ||
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</div> | </div> | ||
</div> | </div> | ||
+ | <div class="frontspace frontfirstwrapper2"> | ||
+ | <h1 id="fronttheplan">The Plan</h1> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <div class="frontheadlines"> | ||
+ | |||
+ | <h2 class="front_phase_headline">PHASE ONE - Preparations on Earth</h2> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
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+ | |||
+ | <div class="container-fluid"> | ||
+ | |||
+ | |||
+ | <!-- PHASE OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOONE --> | ||
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+ | <div class="frontspace frontphases" > | ||
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+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#protectionfromuv"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/b/b4/T--DTU-Denmark--front-icon-11.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">We would begin by genetically optimizing a fungal species for the purpose of making construction materials.</h5> | ||
+ | </div> | ||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#protectionfromuv"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/b/b4/T--DTU-Denmark--front-icon-11.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
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+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Vigorous testing and verification on the fungus would be performed on earth to ensure proper gene function and behavior before launch.</h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Results"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/1/1d/T--DTU-Denmark--front-icon-12.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/d/d1/T--DTU-Denmark--front-icon-13.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Various simulations and models will play a crucial part in designing sustainable structures that can protect humans against the hostile Martian environment.</h5> | ||
+ | </div> | ||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/d/d1/T--DTU-Denmark--front-icon-13.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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+ | <!-- PHASE TWOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO --> | ||
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+ | <div class="frontheadlines"> | ||
+ | <h2 class="front_phase_headline">PHASE TWO - Inventory</h2> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontspace frontphases"> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="frontleft frontcomputer"> | ||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#missionarchitecture"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/9/9f/T--DTU-Denmark--front-icon-21.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">The space shuttle would need to have the essentials for our fungus to be grown. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile" > | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#missionarchitecture"><img class="media object frontpics50 frontlink" src="https://static.igem.org/mediawiki/2018/9/9f/T--DTU-Denmark--front-icon-21.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Vials of spores from our GMO fungus would be prepared and a necessary means of biomass, most likely cyanobacteria, will be included. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#growingfungusonMars"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/5/53/T--DTU-Denmark--front-icon-22.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#buildstructures"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/7/72/T--DTU-Denmark--front-icon-23.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Specific molds will let the fungus grow in a desired shape. Creating the pieces for the dome. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#buildstructures"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/7/72/T--DTU-Denmark--front-icon-23.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
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+ | <div class="frontright"> | ||
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+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">General laboratory tools will have to be included to perform the needed work.</h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Safety"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/1/10/T--DTU-Denmark--front-icon-24.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
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+ | </div> | ||
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+ | <!-- PHASE THREEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE --> | ||
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+ | <div class="frontheadlines"> | ||
+ | <h2 class="front_phase_headline">PHASE THREE - Producing the Sheets</h2> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontspace frontphases"> | ||
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+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2b/T--DTU-Denmark--front-icon-31.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Plate production will be established inside a provisional inflatable tent. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2b/T--DTU-Denmark--front-icon-31.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Biomass and spores will be combined in easy-to-manage molds. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/f/ff/T--DTU-Denmark--front-icon-32.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/9/96/T--DTU-Denmark--front-icon-33.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Growth in a confined space will let the fungus conform into the desired shape. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/GrowthModelling"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/9/96/T--DTU-Denmark--front-icon-33.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">When the mold is appropriately filled, the fungus will be extracted and killed as preparation for the building process.</h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Results-choosing-organism"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/d/db/T--DTU-Denmark--front-icon-34.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
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+ | </div> | ||
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+ | <!-- PHASE FOOOOOOOOOOOOOOOOOOOOOOOOOOUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUR --> | ||
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+ | <div class="frontheadlines"> | ||
+ | <h2 class="front_phase_headline" >PHASE FOUR - Assembly</h2> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontspace frontphases"> | ||
+ | |||
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+ | |||
+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2e/T--DTU-Denmark--front-icon-41.png"></a> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">The produced materials are assembled to create larger habitat structures. Domes will likely be used as these are best at distributing the forces of internal pressure. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/2/2e/T--DTU-Denmark--front-icon-41.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Given the high pressure difference between human habitats and the Martian atmosphere, the final structures will have to be sealed as to make the structure completely airtight. | ||
+ | |||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/StructuralIntegrity"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/d/df/T--DTU-Denmark--front-icon-42.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#melanin"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/a/a1/T--DTU-Denmark--front-icon-43.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">At this point, otherwise optimized fungal species can be used to synthesize a range of useful biochemicals and materials. | ||
+ | </h5> | ||
+ | </div> | ||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#melanin"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/a/a1/T--DTU-Denmark--front-icon-43.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">The cyanobacteria used in the production of fungal materials can also be used to supply oxygen to the habitat.</h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://2018.igem.org/Team:DTU-Denmark/Design#growingfungusonMars"><img class="media object frontpics60 frontlink" src="https://static.igem.org/mediawiki/2018/e/e5/T--DTU-Denmark--front-icon-44.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
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+ | |||
+ | <!-- PHASE FIIIVEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE --> | ||
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+ | <div class="frontheadlines"> | ||
+ | <h2 class="front_phase_headline">Why do we want to land on Mars?</h2> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontspace frontphases"> | ||
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+ | <div class="frontleft"> | ||
+ | <div class="col-xs-2 frontcomputer"> | ||
+ | <a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/8/8e/T--DTU-Denmark--front-icon-51.png"></a> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">The first space race that led to the moon landing was hugely influenced by political currents, but the will to expand is a notion that dates back to the period after the era of imperialism. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2 frontmobile"> | ||
+ | <a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics80 frontlink" src="https://static.igem.org/mediawiki/2018/8/8e/T--DTU-Denmark--front-icon-51.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="frontright"> | ||
+ | |||
+ | <div class="col-xs-10"> | ||
+ | <h5 class="media heading">Many parallels from the moon landing can be drawn to the current race of getting the human race to Mars, where it can be concluded that getting there is inevitable. | ||
+ | </h5> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-xs-2"> | ||
+ | <a href="https://static.igem.org/mediawiki/2018/b/b9/T--DTU-Denmark--colonizing-mars.pdf" target="_blank"><img class="media object frontpics70 frontlink" src="https://static.igem.org/mediawiki/2018/0/07/T--DTU-Denmark--front-icon-52.png"></a> | ||
+ | </div> | ||
+ | </div> | ||
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+ | </div> | ||
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+ | <div class="frontspace frontresume" > | ||
+ | <div class="spacefront"> | ||
+ | <h1 id="frontheadline2">Challenges</h1> | ||
+ | <h5 style="color:#fff;text-align: center;"> Researching and optimizing a fungal species for the purpose of mycotecture. | ||
+ | <br><br> | ||
+ | Designing easily deployable production facilities that can protect the cultivation systems from the Martian environment. | ||
+ | <br><br> | ||
+ | We have not researched whether the structural properties depend on being on earth, eg. lower pressure compromising strength. | ||
+ | <br><br> | ||
+ | </h5> | ||
</div></div> | </div></div> | ||
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+ | <br><br> | ||
+ | <h5> | ||
+ | The team received a bronze medal for their project | ||
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+ | </h5> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/9/93/T--DTU-Denmark--front-bronze.png" style="width: 200px;" ></img> | ||
+ | <p> | ||
+ | <br><br></p> | ||
+ | </div> | ||
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<h5>Useful links</h5> | <h5>Useful links</h5> | ||
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<a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a> | <a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a> | ||
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Latest revision as of 23:15, 6 December 2018
Fungal building materials for extreme environments
Colonization of uninhabitable areas, like Mars, will require building materials to be transported to the site of deployment. Transport limitations such as space and weight make this process very expensive. Based on these challenges, we propose to make building materials from fungal mycelium to be grown on site. Our project is focused on how to optimize the material properties of the fungi through engineering of basic fungal characteristics. Our initial studies identified Aspergillus oryzae as the best candidate chassis for material properties and ease of genetic engineering. Based on our choice of fungus, we decided to increase the gene expression of melanin to improve A. oryzae’s capabilities of withstanding UV radiation and change the colors of the fungi by inserting a blue chromoprotein gene. Furthermore, we have designed a final geometric structure that can withstand external conditions and reduce the amount of work needed to assemble it.
The Plan
PHASE ONE - Preparations on Earth
We would begin by genetically optimizing a fungal species for the purpose of making construction materials.
Vigorous testing and verification on the fungus would be performed on earth to ensure proper gene function and behavior before launch.
PHASE TWO - Inventory
Vials of spores from our GMO fungus would be prepared and a necessary means of biomass, most likely cyanobacteria, will be included.
PHASE THREE - Producing the Sheets
PHASE FOUR - Assembly
The produced materials are assembled to create larger habitat structures. Domes will likely be used as these are best at distributing the forces of internal pressure.
Given the high pressure difference between human habitats and the Martian atmosphere, the final structures will have to be sealed as to make the structure completely airtight.
At this point, otherwise optimized fungal species can be used to synthesize a range of useful biochemicals and materials.
Why do we want to land on Mars?
The first space race that led to the moon landing was hugely influenced by political currents, but the will to expand is a notion that dates back to the period after the era of imperialism.
Challenges
Researching and optimizing a fungal species for the purpose of mycotecture.
Designing easily deployable production facilities that can protect the cultivation systems from the Martian environment.
We have not researched whether the structural properties depend on being on earth, eg. lower pressure compromising strength.
The team received a bronze medal for their project