Difference between revisions of "Team:FJNU-China"
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:FJNU-China/CSS/bootstrap-min?action=raw&ctype=text/css | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:FJNU-China/CSS/bootstrap-min?action=raw&ctype=text/css | ||
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| − | + | <!-- 导航栏 --> | |
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<section class="intro"> | <section class="intro"> | ||
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<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
</button> | </button> | ||
| − | <a class="navbar-brand" href="https://2018.igem.org/Team:FJNU-China""><img src="https://static.igem.org/mediawiki/2018/9/9d/T--FJNU-China--index_logo.png" width="100px" height=" | + | <a class="navbar-brand" href="https://2018.igem.org/Team:FJNU-China""><img src="https://static.igem.org/mediawiki/2018/9/9d/T--FJNU-China--index_logo.png" width="100px" height="80px" alt="FJNU-China " style="margin-top: -5px !important;"></a> |
</div> | </div> | ||
<div class="collapse navbar-collapse nopadding" id="navbar-collapse"> | <div class="collapse navbar-collapse nopadding" id="navbar-collapse"> | ||
<ul class="nav navbar-nav main-menu"> | <ul class="nav navbar-nav main-menu"> | ||
<li class="active"><a href="https://2018.igem.org/Team:FJNU-China">Home</a></li> | <li class="active"><a href="https://2018.igem.org/Team:FJNU-China">Home</a></li> | ||
| − | <li class="clearfix"><a href=" | + | <li class="clearfix"><a href="#">Team</a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2018.igem.org/Team:FJNU-China/Team">About us</a></li> | <li><a href="https://2018.igem.org/Team:FJNU-China/Team">About us</a></li> | ||
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</ul> | </ul> | ||
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| − | <li class="clearfix"><a href=" | + | <li class="clearfix"><a href="#">Projects</a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2018.igem.org/Team:FJNU-China/Description">Description</a></li> | <li><a href="https://2018.igem.org/Team:FJNU-China/Description">Description</a></li> | ||
<li ><a href="https://2018.igem.org/Team:FJNU-China/Design">Design</a></li> | <li ><a href="https://2018.igem.org/Team:FJNU-China/Design">Design</a></li> | ||
<li ><a href="https://2018.igem.org/Team:FJNU-China/Result">Results</a></li> | <li ><a href="https://2018.igem.org/Team:FJNU-China/Result">Results</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:FJNU-China/Applied_Design">Application</a></li> | ||
<li><a href="https://2018.igem.org/Team:FJNU-China/Demonstrate">Demonstrate</a></li> | <li><a href="https://2018.igem.org/Team:FJNU-China/Demonstrate">Demonstrate</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <li class="clearfix"><a href="https://2018.igem.org/Team:FJNU-China/Model">Model</a></li> | |
<li class="clearfix"><a href="#">Laboratory</a> | <li class="clearfix"><a href="#">Laboratory</a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
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</section> | </section> | ||
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<div class="container"> | <div class="container"> | ||
<h1 class="animated fadeInDownBig">A wonderful combination of Bacteriostasis and Aroma Producing</h1> | <h1 class="animated fadeInDownBig">A wonderful combination of Bacteriostasis and Aroma Producing</h1> | ||
| − | <p class="animated fadeInUp">PLA broad spectrum bacteriostatic | + | <p class="animated fadeInUp">PLA, broad spectrum bacteriostatic </br>2-PE, origin of rose fragrance</p> |
| − | <a href="https://2018.igem.org/Team:FJNU-China/ | + | <a href="https://2018.igem.org/Team:FJNU-China/Design" class="animated fadeInUp">Read More</a> |
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<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md- | + | <div class="col-md-4 col-sm-4 tab2-img"> |
| − | <a name="why"> <img src="https://static.igem.org/mediawiki/2018/ | + | <a name="why"> <img src="https://static.igem.org/mediawiki/2018/2/26/T--FJNU-China--index-hand2.png" alt="" class="auto img-responsive center-block" style="width:70%;"></a> |
</div> | </div> | ||
| − | <div class="text col-md- | + | <div class="text col-md-8 col-sm-8 tab2-text"> |
<h3><span style="font-size: 40px">Why</span> do we do this ?</h3> | <h3><span style="font-size: 40px">Why</span> do we do this ?</h3> | ||
<p style="text-align: left;"> According to statistics, the microbes we touched each day is about 3 times more than the human cells. The infection with some specific microbes can cause infectious diseases and give unpleasant smell. Bacteria can infect any area of the body and cause different diseases: pneumonia, meningitis, food poisoning, etc.<br> | <p style="text-align: left;"> According to statistics, the microbes we touched each day is about 3 times more than the human cells. The infection with some specific microbes can cause infectious diseases and give unpleasant smell. Bacteria can infect any area of the body and cause different diseases: pneumonia, meningitis, food poisoning, etc.<br> | ||
| − | We believe that it will be very interesting and meaningful to inhibit most of the bacteria that affects our normal life and to make the environment that has an unusual smell become fragrant in a more efficient, environmentally friendly way.<br> | + | We believe that it will be very interesting and meaningful to<span style="font-weight:bold;"> inhibit most of the bacteria that affects our normal life and to make the environment that has an unusual smell become fragrant</span> in a more efficient, environmentally friendly way.<br> |
| − | Based on the principles of metabolic engineering, we engineered an<span style="font-style:italic;"> E.coli </span>strain producing | + | Based on the principles of metabolic engineering, we engineered an<span style="font-style:italic;"> E.coli </span> strain<span style="font-weight:bold;"> producing phenyllactic acid that has broad-spectrum antibacterial effects, and the rose-like aroma compound 2-phenylethanol</span>.They are all bio-safe substances of food grade. |
| − | We incorporated the common components of temperature and salt control in the synthesis system, which applied | + | We incorporated the common components of temperature and salt control in the synthesis system, which applied phenyllactic acid and 2-phenylethanol to the environment of our daily lives.The environemnt around us will be greatly improved due to the release of phenyllactic acid and 2-phenylethanol. |
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<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md- | + | <div class="col-md-4 col-sm-4"> |
| − | <img src="https://static.igem.org/mediawiki/2018/a/ab/T--FJNU-China--index-PLA.png" alt="" class="auto img-responsive center-block"> | + | <img src="https://static.igem.org/mediawiki/2018/a/ab/T--FJNU-China--index-PLA.png" alt="" class="auto img-responsive center-block"> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/a/a4/T--FJNU-China--index-2-PE.png" alt="" class="auto img-responsive center-block" style="width:70% !important; "> | |
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</div> | </div> | ||
| − | <div class="text col-md- | + | <div class="text col-md-8 col-sm-8"> |
<h3><span style="font-size: 40px">What</span> do we want to do?</h3> | <h3><span style="font-size: 40px">What</span> do we want to do?</h3> | ||
<p class="tab-p">We designed two applications to bring PLA and 2-PE into practice. In order to highlight the role of PLA and 2-PE, broad-spectrum antibacterial and rose-like aroma compound, we add it to solve the internal and external environment. </p> | <p class="tab-p">We designed two applications to bring PLA and 2-PE into practice. In order to highlight the role of PLA and 2-PE, broad-spectrum antibacterial and rose-like aroma compound, we add it to solve the internal and external environment. </p> | ||
| − | <p style="text-align: left;"> Phenyllactic acid（PLA）widely found in kimchi, honey and other foods. | + | <p style="text-align: left;"> Phenyllactic acid（PLA）widely found in kimchi, honey and other foods. It is a new type of natural antibacterial substance and preservative, which can inhibit a series of Gram-negative, positive bacteria and fungi. </br> |
| − | + | In our project, d-lactic acid dehydrogenase (D-ldh) and phenylalanine transaminase (Tyrb) were efficiently expressed in <span style="font-style:italic;">Escherichia coli</span>, besides, glutamate dehydrogenase (GDH) was expressed, for the internal recycling of cofactor NADH. The phenylalanine was converted to phenylpyruvate by the action of an ammonia-transferase (Tyrb), and then dehydrogenated by a lactate dehydrogenase (D-ldh) to produce phenyllactic acid.</br></br> 2-Phenylethanol (2-PE)is one of the most important perfume, which has a roselike quietly elegant, delicate and persistent aroma. In recent years, many studies have shown that phenylethyl alcohol not only has antibacterial activity, but also its phenylethanoid glycoside has medicinal value.</br> | |
| − | + | In our project, we used the <span style="font-style:italic;">tnaA</span> gene deletion strain of <span style="font-style:italic;">Escherichia coli</span> as an engineering strain, and the phenylalanine is converted to phenylpyruvate by the transamination of aromatic transaminase (TyrB), then acetaldehyde is formed by decarboxylation of phenylpyruvate decarboxylase (Aro10), and finally 2-phenylethyl is produced by dehydrogenation of phenyla- cetaldehyde reductase (PAR). The 2-PE biosynthetic pathway was constructed, and finally the metabolic pathway and optimization of the catalytic conditions were carried out to significantly increase the yield of 2-PE. | |
| + | |||
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<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md-6 col-sm-6 tab2-img"> | + | <div class="col-md-6 col-sm-6 tab2-img" style="margin-top:-50px;"> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/5/5c/T--FJNU-China--How_to_apply.png" alt="" class="auto img-responsive center-block"> |
</div> | </div> | ||
<div class="text col-md-6 col-sm-6 tab2-text"> | <div class="text col-md-6 col-sm-6 tab2-text"> | ||
| − | <h3><span style="font-size: 40px">How</span> | + | <h3><span style="font-size: 40px">How</span> to make practical use ?</h3> |
| − | <p style="text-align: left;"> | + | <p style="text-align: left;"> We plan to achieve the practical application of the project through the design of garbage can lid and body lotion.</br> |
| − | We | + | |
| + | The new garbage can lid that we designed can be used to contain our engineered bacteria. With pressing a red button on the top of it, the engineered bacteria in the lid could evenly flow into the can and start expressing PLA and 2PE, so as to inhibit the growth of harmful bacteria and produce rose-like aroma. This application design is currently practiced in the waste liquid cylinder in the laboratory, and we plan to extend it to more areas in our life | ||
| + | .</br> | ||
| + | |||
| + | We used body lotion or spray as our final product to get rid of bromidrosis. Our engineered bacteria will be made into freeze-dried powder and store at room temperature. Just need to add the powder into the body lotion, when the users want to use the body lotion. (Details can be found on our <a href="https://2018.igem.org/Team:FJNU-China/Applied_Design">Application </a>page.)</br> | ||
| + | |||
</p> | </p> | ||
</div> | </div> | ||
Latest revision as of 02:50, 8 December 2018
A wonderful combination of Bacteriostasis and Aroma Producing
PLA, broad spectrum bacteriostatic 2-PE, origin of rose fragrance
Read More
Why do we do this ?
According to statistics, the microbes we touched each day is about 3 times more than the human cells. The infection with some specific microbes can cause infectious diseases and give unpleasant smell. Bacteria can infect any area of the body and cause different diseases: pneumonia, meningitis, food poisoning, etc.
We believe that it will be very interesting and meaningful to inhibit most of the bacteria that affects our normal life and to make the environment that has an unusual smell become fragrant in a more efficient, environmentally friendly way.
Based on the principles of metabolic engineering, we engineered an E.coli strain producing phenyllactic acid that has broad-spectrum antibacterial effects, and the rose-like aroma compound 2-phenylethanol.They are all bio-safe substances of food grade.
We incorporated the common components of temperature and salt control in the synthesis system, which applied phenyllactic acid and 2-phenylethanol to the environment of our daily lives.The environemnt around us will be greatly improved due to the release of phenyllactic acid and 2-phenylethanol.
What do we want to do?
We designed two applications to bring PLA and 2-PE into practice. In order to highlight the role of PLA and 2-PE, broad-spectrum antibacterial and rose-like aroma compound, we add it to solve the internal and external environment.
Phenyllactic acid（PLA）widely found in kimchi, honey and other foods. It is a new type of natural antibacterial substance and preservative, which can inhibit a series of Gram-negative, positive bacteria and fungi. In our project, d-lactic acid dehydrogenase (D-ldh) and phenylalanine transaminase (Tyrb) were efficiently expressed in Escherichia coli, besides, glutamate dehydrogenase (GDH) was expressed, for the internal recycling of cofactor NADH. The phenylalanine was converted to phenylpyruvate by the action of an ammonia-transferase (Tyrb), and then dehydrogenated by a lactate dehydrogenase (D-ldh) to produce phenyllactic acid. 2-Phenylethanol (2-PE)is one of the most important perfume, which has a roselike quietly elegant, delicate and persistent aroma. In recent years, many studies have shown that phenylethyl alcohol not only has antibacterial activity, but also its phenylethanoid glycoside has medicinal value. In our project, we used the tnaA gene deletion strain of Escherichia coli as an engineering strain, and the phenylalanine is converted to phenylpyruvate by the transamination of aromatic transaminase (TyrB), then acetaldehyde is formed by decarboxylation of phenylpyruvate decarboxylase (Aro10), and finally 2-phenylethyl is produced by dehydrogenation of phenyla- cetaldehyde reductase (PAR). The 2-PE biosynthetic pathway was constructed, and finally the metabolic pathway and optimization of the catalytic conditions were carried out to significantly increase the yield of 2-PE.
How to make practical use ?
We plan to achieve the practical application of the project through the design of garbage can lid and body lotion. The new garbage can lid that we designed can be used to contain our engineered bacteria. With pressing a red button on the top of it, the engineered bacteria in the lid could evenly flow into the can and start expressing PLA and 2PE, so as to inhibit the growth of harmful bacteria and produce rose-like aroma. This application design is currently practiced in the waste liquid cylinder in the laboratory, and we plan to extend it to more areas in our life . We used body lotion or spray as our final product to get rid of bromidrosis. Our engineered bacteria will be made into freeze-dried powder and store at room temperature. Just need to add the powder into the body lotion, when the users want to use the body lotion. (Details can be found on our Application page.)