Difference between revisions of "Safety/Final Safety Form"

 
 
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<div class="column full_size">
 
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<h1>Safety Form</h1>
 
<h1>Safety Form</h1>
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</div>
  
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<p>This form is for you to tell us all about your project, the organisms/parts you are using, the potential risks of your project, and what you are doing to reduce those risks.</p>
 
  
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<ul>
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<li><strong>We encourage STUDENTS, instead of instructors, to complete this form.</strong></li>  
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<div class="column quarter_size">
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        <li>You will need an Instructor or PI to sign and submit this form by the October 2 deadline in order to be qualified for award and prizes at the Giant Jamboree.</li>
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<img src="https://static.igem.org/mediawiki/2018/f/f7/Safety_form.svg" height="150px">
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        <li>As you type, this form will remember your answers - you do not need to click "Submit" until Parts 1-5 have been completed.</li>
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</ul>
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<div class="column three_quarter_size">
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<p>The purpose of this form is for everyone, including iGEM, to know you are doing your project safely and securely. This is how we know that everyone in iGEM is assessing and managing any risks to yourselves, your colleagues, communities or the environment. If you don't tell us how you are dealing with these risks, we don't know. Being transparent about any possible risks and how we are managing them is a key component of being a responsible scientist or engineer - so please answer the questions as fully as possible. </p>
 
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<h2> Deadlines </h2>
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<div class="highlight gray decoration_red_top">
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<p>If you will not be able to complete this form before the deadline, please email us (safety AT igem DOT org) and tell us about your situation.</p>
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<h3>Important</h3>
 +
<p>Complying with the safety and security requirements is a condition of competing at iGEM - that includes providing all the information in this form by the relevant deadlines. <span class="red_text">Failure to do so can lead to immediate disqualification from the competition and referral to the Responsible Conduct Committee.</span></p>
 
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<h3>Deadlines</h3>
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<p>The form should be updated throughout the project whenever there is a change that alters possible risks or the procedures, practices and containment provisions being used to manage them.
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</p>
  
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<div class="column half_size">
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<img src="https://static.igem.org/mediawiki/2018/5/56/Safety_form_team_fill.svg" height="100px">
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<h4> June 28th </h4>
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<p> An initial version of this form, with as much information as possible, is to be provided by the team by <a href="https://2019.igem.org/Calendar">June 28th</a>. The form should NOT be signed off by the PI. </p>
  
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<h4> Complete by Friday, June 30, 2018 </h4>
 
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<ul>
 
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<li> Part 1: Contact </li>
 
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<li>Part 2: About our Lab  </li>
 
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<li>Part 3: About our Project </li>
 
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<li><i>Note: you do not need to click "Submit". The form will automatically save your responses.</i></li>
 
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</ul>
 
 
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</div>
  
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<div class="column half_size" >
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<div class="column half_size">
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<h4> Complete by Monday, October 2, 2018 </h4>
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<img src="https://static.igem.org/mediawiki/2018/8/8b/Safety_form_pi_submit.svg" height="100px">
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<ul>
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<h4>September 13th</h4>
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<li>Part 4: New Parts </li>
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<p>
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<li>Part 5: Sign Off <i> submitted by the Instructor or PI </i>
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A final version of this form must be signed off by the PI by <a href="https://2019.igem.org/Calendar">September 13th</a>.<br>
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</ul>
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<small> (Please note, that you will need to plan ahead - your PI being unavailable or on vacation is not an acceptable excuse for late submission of this form)</small>
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</p>
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</div>
 
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<div class="column half_size">
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<h3>Approach</h3>
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<p>In general, we are looking for information in three areas. We want you to explain: </p>
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 +
<ol>
 +
<li>What risks might be connected to your project now or in the future (Part 1); </li>
 +
<li>What procedures, practices and containment provisions you are using to manage the risks you identify (Part 2); and </li>
 +
<li>That you are complying with iGEM’s safety and security <a href="https://2018.igem.org/Safety/Rules">rules</a> and <a href="https://2018.igem.org/Safety/Policies">policies</a> (Part 3).</li>
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<h2>Safety Insert Survey</h2>
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<p>
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This year, we included a Safety Insert in the Distribution Kit. We are asking each team to complete this brief survey about the insert. Please only submit one answer per team.
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</p>
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<div class="button">
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<a href="https://www.surveymonkey.co.uk/r/NJF25PX">
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SAFETY INSERT SURVEY
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</a>
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<h3>Other required safety and security information</h3>
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<h4> NOTE </H4>
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<p>Any time you are planning to use an organism or part not on the <a href="https://2018.igem.org/Safety/White_List">White List</a> the team needs to submit a <a href="https://2018.igem.org/Safety/Check_In">Check-in Form</a> <strong>before</strong> using them. <br><br>
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<p>
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Any time you are planning to use a vertebrate animal in your project the team needs to submit an <a href="https://2018.igem.org/Safety/Animal_Use_Check_In">Animal Use Form</a> <strong>before</strong> using them.
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Complying with the safety requirements is a condition of competing at iGEM - that includes providing all the information in this form by the relevant deadlines. <br> Failure to do so can lead to immediate disqualification from the competition and referral to the Responsible Conduct Committee.
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<h2> Team Selection </h2>  
 
<h2> Team Selection </h2>  
  
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<!-- <span id="form_title_display"></span> --> <span id="owner_name_display"></span> <span id="prompt_to_choose_team"> Please choose a team</span>
 
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<h4>  This form has been submitted.</H4>
 
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<p>You can <a href="#" id="popwin_unsubmit">unsubmit the form</a> if you wish to make further edits.</p>
 
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<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
 
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CONTACT INFORMATION
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OVERVIEW
  
 
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<h2>1.-  Contact Information </h2>  
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<h2>Overview </h2>
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<p>Name <input type="text" name="Team member Name" data-form-field="corr_team_member_name" placeholder="Name"></p>
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<p>Email <input type="text" name="Team member Email" data-validate-as="email" class="validate-on-change" data-form-field="corr_team_member_email" placeholder="Email"></span></p>
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<p class="question">Contact Information</p>
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<p class="question"> Name <input type="text" name="Team member Name" data-validate-as="check_characters" class="validate-on-change" data-form-field="corr_team_member_name" placeholder="Name"></p>
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<p class="question"> Email <input type="text" name="Team member Email" data-validate-as="email" class="validate-on-change" data-form-field="corr_team_member_email" placeholder="Email"></span></p>
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<!--------------------------------------------------------------------------------------------- Question 1 --------------------------------------------------------------------------------------------------------->
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<p class="question"> 1. Please upload a photo or two of your lab to the iGEM 2018 server (include your team name in the file name), preferably showing the relevant safety features and paste the link here: </p>
 +
 
 +
<p class="note">(Instructions on how to upload an image to our servers can be found <a href="https://2018.igem.org/Resources/Wiki_Editing_Help">here</a>)</p>
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<textarea rows="2" name="Lab Photograph" data-form-field="lab_photo"></textarea>
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<!--------------------------------------------------------------------------------------------- Question 2 --------------------------------------------------------------------------------------------------------->
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<div class="column full_size">
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<p class="question"> 2. Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. </p>
 +
<p class="note">(Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)</p>
 +
 +
<textarea rows="6" name="Describe Project" data-form-field="project_description"></textarea>
 +
 
</div>
 
</div>
  
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<div class="column half_size message_box">
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<h4> Note </h4>
+
 
−
<p>It is your responsibility to demonstrate that your work is safe. The easiest way to do this is through a well-researched and comprehensive safety form.</p>
+
<div class="column full_size">
 +
 
 +
<p class="note"> <b> Describe your project </b><br>
 +
This part of the form is for you to tell us about your primary project idea, what organisms and parts you will use and what your project will do. We know that iGEM teams often change project topics during the competition. When you change your primary project idea, please update this form to tell us about your new idea! If you are still working on multiple project ideas by June 29, you may choose one to describe on this form. If there are major changes to your project after June 29, please contact the Safety and Security Committee by emailing safety AT igem DOT org.  
 +
</p>
 
</div>
 
</div>
 +
 +
<div class="column half_size">
 +
 +
<p class="note">
 +
<b> Good example answers </b> <br>
 +
"Our bacteria will be engineered to interact with human cells. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."<br>
 +
"Our algae will receive gasses high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2 from the gas."
 +
</p>
 +
 +
</div>
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 +
<div class="column half_size">
 +
<p class="note"> <b> Bad example answers (not enough detail) </b><br>
 +
"We are engineering E. coli to cure liver cancer."
 +
<br>
 +
"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."</p>
 +
 +
</div>
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 +
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<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
 
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ABOUT OUR LAB
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Part 1 - Risks
  
 
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<fieldset>
 
<fieldset>
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<h2>2.- About our Lab </h2>
 
−
<p> This part of the form is for you to tell us about your laboratory space and your safety equipment. If you need help, please consult your faculty advisor or laboratory manager. You can also read the <a href="https://2018.igem.org/Safety">Safety page</a> and the <a href="https://2018.igem.org/Safety/Risk_Groups">Risk Groups page</a> for basic information about different types of biology labs. </p>
 
  
−
<!--------------------------------------------------------------------------------------------- Question 1 --------------------------------------------------------------------------------------------------------->
 
  
 +
<div class="column full_size">
  
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<p class="question">1. Please upload a photo or two of your lab to the iGEM 2018 server (include your team name in the file name), preferably showing the relevant safety features and paste the link here:</p>
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<h2>1. Identifying possible risks </h2>
−
<textarea rows="1" name="Lab Photograph" data-form-field="lab_photo"></textarea>
+
  
  
−
<!--------------------------------------------------------------------------------------------- Question 2 --------------------------------------------------------------------------------------------------------->
 
  
 +
<p class="note">iGEM has developed a <a href="https://2018.igem.org/Safety/Risk_Assessment_Tool"> Risk Assessment Tool </a> to help you identify possible risks to you, your colleagues, communities, or the environment. We encourage you to use this tool before filling in this part of the form.
 +
What are you using / planning to use?
 +
Some organisms and parts present risks beyond what is ordinary for lab work in synthetic biology. As your project progresses, you should consider the risks presented by each organism and part you plan to use.
 +
</p>
  
−
<br><br><br>
 
  
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<h3> Biosafety Level </h3>
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<!--------------------------------------------------------------------------------------------- Question 3 --------------------------------------------------------------------------------------------------------->
−
<p class="question">2. What is the Safety Level of your lab? <a target="_blank" href="https://2018.igem.org/Safety/Risk_Groups">[Help about Risk Groups and Safety Levels]</a></p>
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<div class="column half_size">
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<p class="question"> 3. Which whole organisms, including viruses, are you planning to use or using in your project?</p>
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<ul>
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<p class="note">Please provide as much detail as possible (such as strain information). If you are not using an organism, please note this.</p>
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<li><label><input type="radio" name="lab_safety_level" data-form-field="lab_bsl" value="bsl_1"> Level 1 (low risk)</label></li>
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<li><label><input type="radio" name="lab_safety_level"  data-form-field="lab_bsl" value="bsl_2"> Level 2 (moderate risk)</label></li>
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<li style="color: #a0a0a0;"><label><span class="wrapper"><input type="radio" disabled id="bsl_3"></span> Level 3 (high risk)</label></li>
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<li style="color: #a0a0a0;"><label><span class="wrapper"><input type="radio" disabled id="bsl_4"></span> Level 4 (extreme risk)</label></li>
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<li><label><input type="radio"  name="lab_safety_level" data-form-field="lab_bsl" value="bsl_none"> Our team is not doing any wet-lab work</label></li>
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</ul>
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</div>
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<div class="column half_size message_box">
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<textarea rows="5" name="which organisms" data-form-field="which_organisms"></textarea>
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<h4> Note </h4>
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<p>
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−
iGEM teams should not use Risk Group 3 or 4 organisms, and they should not work in Safety Level 3 or 4 labs.
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If you are planning to work at Safety Level 3 or 4, contact safety (AT) igem (DOT) org right away!!
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<li><label><input type="radio" name="lab_safety_level"  data-form-field="lab_bsl" value="bsl_other"> Other safety level:</label> <textarea rows="2" name="Other safety level (describe)" data-form-field="bsl_other_describe" data-validation="[value=bsl_other]" placeholder="Please describe"></textarea></li>
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<li><label><input type="radio" name="lab_safety_level" data-form-field="lab_bsl" value="bsl_multiple"> We have several different lab spaces with different Safety Levels:</label> <textarea rows="2" name="Different safety levels (describe)" data-form-field="bsl_multiple_describe" data-validation="[value=bsl_multiple]" placeholder=" Please describe what experiments you do in each space"></textarea></li>
 
  
  
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<li><label><input type="radio"name="lab_safety_level"  data-form-field="lab_bsl" value="bsl_unknown"> Unknown (please comment):</label> <textarea rows="2" name="Safety level unknown (comment)" data-form-field="bsl_unknown_describe" data-validation="[value=bsl_unknown]" placeholder="Please describe"></textarea></li>
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<!--------------------------------------------------------------------------------------------- Question 4 --------------------------------------------------------------------------------------------------------->
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<p class="question"> 4. What risks could these organisms pose to you or your colleagues, or to your community or the environment if they escape the lab?</p>
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<p class="note">If you are not using an organism, please note this.</p>
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<textarea rows="5" name="what risk organisms pose" data-form-field="what_risk_organism_pose"></textarea>
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<!--------------------------------------------------------------------------------------------- Question 5 --------------------------------------------------------------------------------------------------------->
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 +
<p class="question"> 5. Check all species you are using as a chassis in your project.</p>
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 +
<p class="note"><b>What is your chassis organism?</b> <br>For the purposes of iGEM, a chassis is the organism in which you are putting your parts, or which you are modifying in your project. Many teams will use a common lab organism as a chassis. Some teams may use a more exotic organism. Some project may not involve a chassis.</p>
  
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<!--------------------------------------------------------------------------------------------- Question 3 --------------------------------------------------------------------------------------------------------->
 
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<br><br><br>
 
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<h3> Work areas </h3>
 
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<p class="question"> 3 Which work areas do you use to handle biological materials? Please check all that apply.</p>
 
  
 
<ul>
 
<ul>
−
<li><label><input type="checkbox" name="workarea" data-form-field="workarea_open_bench" value="open_bench"> Open bench </label></li>
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<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_ecoli" value="ecoli"> Escherichia coli (give names of all strains you are using) </label>
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<li><label><input type="checkbox"  name="workarea" data-form-field="workarea_cabinet" value="cabinet"> Biosafety cabinet </label></li>
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<textarea rows="1" name="chassis e.coli" data-form-field="chassis_ecoli_describe" data-validation="[data-form-field=chassis_ecoli]" placeholder="Please comment"></textarea></li>
 +
 +
<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_yeast" value="yeast"> Yeast (Saccharomyces cerevisiae) </label></li>
 +
<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_lactobacillus" value="lactobacillus"> Lactobacillus spp. </label></li>
 +
<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_bacillus" value="bacillus_subtilis"> Bacillus subtilis </label></li>
 +
 +
<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_other" value="other"> Others (give species names) </label>
 +
<textarea rows="1" name="chassis other" data-form-field="chassis_other_describe" data-validation="[data-form-field=chassis_other]" placeholder="Please comment"></textarea></li>
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 +
<li><label><input type="checkbox" name="chassis_used" data-form-field="chassis_no_organism" value="no_organism"> No chassis organism (please comment) </label>
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<textarea rows="1" name="chassis no organism" data-form-field="chassis_no_organism_describe" data-validation="[data-form-field=chassis_no_organism]" placeholder="Please comment"></textarea></li>
  
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<li><label><input type="checkbox" name="workarea"  data-form-field="workarea_other_work" value="other_work"> Other work area: </label> <textarea rows="2" name="Other_work_area_describe" data-form-field="workarea" data-validation="[data-form-field=workarea_other_work]" placeholder="Please describe"></textarea></li>
+
</ul>
  
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<li><label><input type="checkbox" name="workarea"  data-form-field="workarea_unknown" value="work_unknown"> Unknown: </label> <textarea rows="2" name="Work_area_unknown_descrip" data-form-field="workarea_descrip" data-validation="[data-form-field=workarea_unknown]" placeholder="Please comment"></textarea></li>
+
<p class="question">Comments:</p>
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</ul>
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<textarea rows="5" name="chassis used comments" data-form-field="chassis_used_comments"></textarea>
  
  
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<!--------------------------------------------------------------------------------------------- Question 4 --------------------------------------------------------------------------------------------------------->
 
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<bR><bR><br>
 
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<h3> Biosafety Training </h3>
 
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<p class="question">4. Have your team members received any safety training yet?</p>
 
  
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<!--------------------------------------------------------------------------------------------- Question 6 --------------------------------------------------------------------------------------------------------->
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 +
<p class="question"> 6. What risks could your chassis pose to you or your colleagues, or to your community or the environment if they escape the lab? </p>
 +
<p class="note">If not using a chassis organism, please note this.</p>
 +
 
 +
<textarea rows="5" name="risk organisms pose if leave lab" data-form-field="what_risk_organism_pose_if_leave_lab"></textarea>
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<!--------------------------------------------------------------------------------------------- Question 7 --------------------------------------------------------------------------------------------------------->
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 +
<p class="note"><b>What parts are you making or planning to make?</b> <br>
 +
This part of the of the form is for you to tell us about the parts you are planning to make or have developed during your project.
 +
It summarises information that might already have been submitted through <a href="https://2018.igem.org/Safety/Check_In">Check-in forms</a>.
 +
If you submitted a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a> for an organism or part, you should still include it in this section. You may omit non-protein-coding parts (except if they are known virulence or anti-microbial factors – you should undertake a literature search to determine if they are), and you may omit parts that were already in the Registry if you are using them without significant modifications.
 +
For more information on virulence factors see the <a href="https://2018.igem.org/Safety/Policies">Safety Policy</a> page and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. If there are major changes to your project after June 29, please contact the Safety and Security Committee by emailing safety AT igem DOT org.
 +
</p>
 +
 
 +
 
 +
<p class="question"> 7. As part of your project, are you are planning to make / have made new parts or substantively changed existing parts in the Registry. </p>
 +
 
 
<ul>
 
<ul>
−
<li><input type="radio" name="safety_training" data-form-field="safety_training" value="training_already"> Yes, we have already received safety training.</label></li>
+
<li><label><input type="radio" name="change_parts_registry" data-form-field="change_parts_registry" value="change_parts_registry_yes"> Yes (All relevant new or revised parts should be described on a spreadsheet)</label></li>
 +
<li><label><input type="radio" name="change_parts_registry" data-form-field="change_parts_registry" value="change_parts_registry_no"> No </label></li>
 +
</ul>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 
 
  
−
<li><input type="radio" name="safety_training"  data-form-field="safety_training" value="training_notyet"> We plan to receive safety training in the future: </label> <textarea rows="2" name="Plan to receive safety training in future (when?)" data-form-field="training_notyet_describe" data-validation="[value=training_notyet]" placeholder="Please specify approximately when"></textarea></li>
 
  
−
<li><input type="radio" name="safety_training" data-form-field="safety_training" value="training_none"> We will not have safety training:</label> <textarea rows="2" name="No safety training (comment)" data-form-field="training_none_describe" data-validation="[value=training_none]" placeholder="Please comment"></textarea></li>
+
<!--------------------------------------------------------------------------------------------- Question 8 --------------------------------------------------------------------------------------------------------->
−
</ul>
+
 
 +
<p class="question">8. Part information is submitted in a spreadsheet. </p>
 +
<p class="note">
 +
Please visit this page to download a blank copy of the spreadsheet for this question. (If you need a CSV version instead of XLSX, visit this page.)
 +
</p>
 +
 
 +
<div class="button">
 +
<a href="https://static.igem.org/mediawiki/2018/0/0e/TeamName_Safety2018_Spreadsheet.xlsx">
 +
DOWNLOAD SPREADSHEET
 +
</a>
 
</div>
 
</div>
  
−
<div class="column half_size message_box">
+
 
−
<h4> Note </h4>
+
 
−
<p>
+
 
−
For the purposes of iGEM, biosafety and biosecurity training covers the procedures and practices used to manage risks to your team, colleagues and institution, communities and the environment - from accidents or deliberate misuse of your projects. All team members are expected to be aware of these risks and to work to manage them
+
<p class="note">
 +
<b>Instructions for parts spreadsheet:</b>
 +
Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".
 
</p>
 
</p>
 +
 +
 +
<p class="note">
 +
<b>A. Species name (including strain): </b> For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.
 +
</p>
 +
 +
<p class="note">
 +
<b>B. Risk Group:</b> Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)
 +
</p>
 +
 +
<p class="note">
 +
<b>C. Risk Group Source: </b>Cite the source from which you obtained the Risk Group information. See <a href="https://2018.igem.org/Safety/Risk_Groups#HowToFindRiskGroup">Risk Group Guide</a> for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.
 +
</p>
 +
 +
<p class="note">
 +
<b>D. Disease risk to humans:</b> Does this organism cause any disease in humans? If yes, what disease does it cause?
 +
</p>
 +
 +
<p class="note">
 +
<b>E. Disease or other risks to the environment:</b> Does the organism cause plant or animal diseases or would in any other way pose a risk to the environment if accidentally released?
 +
</p>
 +
 +
<p class="note">
 +
<b>F. Part number/name:</b>For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.
 +
</p>
 +
 +
<p class="note">
 +
<b>G. Natural function of part:</b> For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.
 +
</p>
 +
 +
<p class="note">
 +
<b>H.How did you acquire it:</b> Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)
 +
</p>
 +
 +
<p class="note">
 +
<b>I. How will you use it:</b> Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")
 +
</p>
 +
 +
<p class="note">
 +
<b>J. Notes: </b> Use this column to give any additional information that is necessary.
 +
</p>
 +
 +
 +
 +
 +
<p class="note"><B>Upload Spreadsheet</B> Please do not change the "Destination Filename"! [File:T--TeamName--Safety2018_Spreadsheet.xls]
 +
You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.
 +
</p>
 +
 +
 +
 +
<div class="button">
 +
<a href="" target="_blank" id="spreadsheet_upload">
 +
UPLOAD SPREADSHEET
 +
</a>
 
</div>
 
</div>
  
  
−
<!--------------------------------------------------------------------------------------------- Question 5  --------------------------------------------------------------------------------------------------------->
 
  
−
<div class="clear"></div>
 
  
−
<p class="question">5. Please briefly describe the topics that you learned about (or will learn about) in your safety training.</p>
 
  
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
  
  
−
<ul>
+
<!--------------------------------------------------------------------------------------------- Question 9 --------------------------------------------------------------------------------------------------------->
−
<li><input type="checkbox"  name="learn" data-form-field="learn_lab_access" value="learn_lab_access_and_rules"> Lab access and rules (including appropriate clothing, eating and drinking, etc.) </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_responsible" value="learn_responsible"> Responsible individuals (such as lab or departmental specialist or institutional biosafety officer) </label></li>
+
−
<li><input type="checkbox"  name ="learn" data-form-field="learn_biosafety_lvl" value="learn_biosafety_levels">Differences between biosafety levels </label></li>
+
−
<li><input type="checkbox"  name="learn" data-form-field="learn_biosafety_equip" value="learn_biosafety_equipment">Biosafety equipment (such as biosafety cabinets) </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_biosafety_microbal" value="learn_biosafety_good_microbal_technique"> Good microbial technique (such as lab practices)</label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_disinfection" value="learn_disinfection"> Disinfection and sterilization </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_emergency" value="learn_emergency"> Emergency procedures </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_transport" value="learn_transport"> Transport rules </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_fire_elect" value="learn_chemicals_fire_electrical">Chemicals, fire and electrical safety </label></li>
+
−
<li><input type="checkbox" name="learn" data-form-field="learn_other_m" value="learn_other"> We will not have safety training: </label> <textarea rows="2" name="learn" data-form-field="learn_other_describe" data-validation="[data-form-field=learn_other_m]" placeholder="Please comment"></textarea></li>
+
  
−
</ul>
 
  
  
 +
 +
<p class="question"> 9. What experiments will you do with your organisms and parts? </p>
  
−
<!--------------------------------------------------------------------------------------------- Question 6  --------------------------------------------------------------------------------------------------------->
+
<p class="note">Please explain briefly. We are particularly keen to understand the boundaries or scope of your project. You should include the names of species / cell lines / strains. You should include experiments involving parts taken from other organisms, even if they are being synthesized rather than isolated from nature – you need not include any parts already in the registry.</P>  
  
 +
<textarea rows="5" name="what_experiments_with_organism_parts" data-form-field="what_experiments_with_organism_parts"></textarea>
  
−
<p class="question"> 6.  Who provided your safety training? Did you get training from multiple people? </p>
 
  
 +
<p class="note">
 +
<b> Example answers </b> <br>
 +
"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."<br>
 +
"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."<br>
 +
"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."<br>
 +
“We are interested in a RNA-binding protein expressed in Kluyveromyces lactis. We have found the sequence in a paper and will have it synthesized by a provider.”
 +
</p>
  
−
<ul>
 
−
<li><input type="checkbox" name="training_who"  data-form-field="training_who_university" value="training_who_university"> University biosafety office </label></li>
 
−
<li><input type="checkbox" name="training_who"  data-form-field="training_who_department" value="training_who_department"> Departmental specialist </label></li>
 
−
<li><input type="checkbox" name="training_who"  data-form-field="training_who_pi" value="training_who_pi"> PIs/instructors </label></li>
 
−
<li><input type="checkbox" name="training_who"  data-form-field="training_who_other" value="training_who_other"> Other </label> <textarea rows="1" name="training_who_descrip" data-form-field="training_who_describe" data-validation="[data-form-field=training_who_other]" placeholder="Please comment"></textarea></li>
 
−
</ul>
 
  
  
−
<!--------------------------------------------------------------------------------------------- Question 7  --------------------------------------------------------------------------------------------------------->
 
  
−
<br><br><br>
 
−
<h3> Oversight </h3>
 
  
−
<p class="question">7. Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety? Please give a link to these guidelines, or briefly describe them if you cannot give a link.</p>
+
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
  
−
<textarea rows="3" name="7. Your institution's biosafety" data-form-field="institution_biosafety"></textarea>
 
  
 +
<!--------------------------------------------------------------------------------------------- Question 10 --------------------------------------------------------------------------------------------------------->
  
 +
<p class="question"> 10. What risks could arise from these experiments? </p>
 +
<p class="note">For example, could they produce aerosols making it more likely that you could inhale something? Or are you using needles and could accidentally stick yourself? Could you produce something that is not inactivated using standard lab protocols? If you are not conducting any experiments, please note this.</p>
  
−
<!--------------------------------------------------------------------------------------------- Question 8  --------------------------------------------------------------------------------------------------------->
+
<textarea rows="5" name="potential risk from experiments" data-form-field="potential_risk_from_experiments"></textarea>
  
  
−
<p class="question">8. In your country / region, what are the laws and regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</p>
 
  
−
<textarea rows="3" name="8. Your country's biosafety laws" data-form-field="country_biosafety"></textarea>
+
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
  
  
 +
<!--------------------------------------------------------------------------------------------- Question 11 --------------------------------------------------------------------------------------------------------->
  
−
<!--------------------------------------------------------------------------------------------- Question 9  --------------------------------------------------------------------------------------------------------->
+
<p class="question">11. Imagine that your project was fully developed into a real product that real people could use. How would people use it? </p>
−
<br><br><br>
+
<p class="note">Check all appropriate boxes and expand in the comments section.
 +
(Note: iGEM teams should not release modified organisms into the natural environment but you could imagine such a release if your project was fully developed.)  </p>
  
−
<h3> Uncertainties </h3>
 
  
−
<p class="question">9. Are there any areas where you have encountered uncertainties concerning institutional, regional, national or international rules and regulations and whether they are relevant to your lab and/or work?</p>
+
<ul>
  
−
<textarea rows="3" name="9. institutional uncertainties" data-form-field="institutional_uncertainties"></textarea>
+
<li><label><input type="checkbox" name="project_product_use" data-form-field="product_foundational" value="foundational"> Our project is foundational / we do not have a specific real-world application in mind
 +
(Examples: library of standardized promoters, system for communication between cells) </label></li>
 +
 +
<li><label><input type="checkbox" name="project_product_use" data-form-field="product_lab_only" value="only_lab"> Only in the lab (Examples: reporter strain for measuring the strength of promoters)</label></li>
 +
 +
  <li><label><input type="checkbox" name="project_product_use" data-form-field="product_factory" value="factory"> In a factory (Examples: cells that make a flavor chemical for food, cells that make biofuel)</label></li>
 +
 +
  <li><label><input type="checkbox" name="project_product_use" data-form-field="product_consumer" value="consumer"> In a consumer product that ordinary people buy (Examples: cells that clean your clothes, bread made with engineered yeast)</label></li>
 +
 +
    <li><label><input type="checkbox" name="project_product_use" data-form-field="product_agriculture_farm" value="agriculture_farm">In agriculture / on a farm (Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)</label></li>
 +
   
 +
    <li><label><input type="checkbox" name="project_product_use" data-form-field="product_small_device" value="small_device"> In a small enclosed device (Examples: a bio-sensing strip with cells that detect arsenic)</label></li>
 +
   
 +
    <li><label><input type="checkbox" name="project_product_use" data-form-field="product_natural_environment" value="natural_environment"> In the natural environment (Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought) </label></li>
 +
   
 +
    <li><label><input type="checkbox" name="project_product_use" data-form-field="product_human_body_or_food" value="human_body_or_food"> To be used in the human body, or in food (Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants) </label></li>
 +
   
 +
<li><label><input type="checkbox" name="project_product_use" data-form-field="product_other" value="other"> Other (Examples: bacteria that live on Mars, or a software project)  </label>
 +
<textarea rows="1" name="describe product use" data-form-field="product_use_describe" data-validation="[data-form-field=product_other]" placeholder="Please comment"></textarea></li>
 +
 +
 +
</ul>
  
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
  
  
 +
<!--------------------------------------------------------------------------------------------- Question 12 --------------------------------------------------------------------------------------------------------->
  
−
<!-------------------------------------------------------------------------------------------- Question 10  --------------------------------------------------------------------------------------------------------->
 
  
−
<p class="question">10. Do you think the design of current regulations is sufficient to ensure safe and ethical practices? </p>
 
  
−
<p>
+
<p class="question">12. What safety, security or ethical risks would be involved with such a use?</p>
−
(If not, how else could you approach the design? We’re interested in your ideas for strategies that could be used to promote safe and ethical practices as it becomes easier to engineer biology (i.e. monitoring people or information, building safety into the design of equipment, etc). Can you think of any useful examples from other fields?)
+
<p class="note"> It is possible that software projects could also pose relevant risks. </p>
−
</p>
+
  
−
<textarea rows="3" name="10. are current regulations sufficient " data-form-field="sufficient_regulations"></textarea>
+
<textarea rows="5" name="safety security or ethical risks " data-form-field="safety_security_ethical_risks"></textarea>
  
  
−
</fieldset>
 
  
 +
</div>
  
 +
 +
</fieldset>
  
  
Line 379: Line 614:
 
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
 
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
  
−
PROJECT OVERVIEW
+
Part 2 - Managing risks
  
 
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
  
 
<fieldset>
 
<fieldset>
−
<h2>3.- About our Project </h2>
+
<div class="column full_size">
−
<p> This part of the form is for you to tell us about your primary project idea, what organisms and parts you will use and what your project will do. We know that iGEM teams often change project topics during the summer. When you change your primary project idea, please update this form to tell us about your new idea!
+
 
−
If you are still working on multiple project ideas by June 30, you may choose one to describe on this form, or you may tell us about other ideas in the "Further Comments" section.
+
<h2>2. Managing the risks you have identified</h2>
 +
 
 +
 
 +
<p class="note">
 +
It is impossible to remove all risk from any biological activity. Instead, we attempt to manage these risks - to reduce them to an acceptable level. In this section, you should outline what steps have been taken (or you are planning to take) to reduce any risks you identified in the first part of the form.
 
</p>
 
</p>
  
  
  
−
<!-------------------------------------------------------------------------------------------- Question 11  --------------------------------------------------------------------------------------------------------->
 
  
−
<br><br><br>
+
<!--------------------------------------------------------------------------------------------- Question 13 --------------------------------------------------------------------------------------------------------->
−
<h3> Project Overview </h3>
+
  
−
<p class="question">11. How will your project work?</p>
 
−
<p>Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)</p>
 
  
  
−
<br><br>
+
<p class="question">13. How will experts overseeing your project help to manage any of the risks you identified in this form?</p>
  
−
<div class="column half_size">
+
<p class="note">
−
<h5>Good example answers:</h5>
+
For example, who is responsible for the safety and security of biology labs at your institution? What role has any institutional biosafety officer played in reviewing your work? What skills do these experts have that might help? For example, do they have a long history of working with an organism or part that might pose a risk? How familiar are they with the experimental procedures and practices you will be, or are, using?
 +
</p>
  
−
+
<textarea rows="5" name="how will experts help manage risks identified" data-form-field="experts_help_manage_risks"></textarea>
−
<p>"Our bacteria will be engineered to interact with human cells. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."</p>
+
  
−
<p>"Our algae will receive gasses high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2 from the gas."</p>
 
  
−
</div>
 
  
  
−
<div class="column half_size">
 
−
<h5>Bad example answers (not enough detail):</h5>
 
  
−
<p>"We are engineering E. coli to cure liver cancer."</p>
+
<div class="clear extra_space"></div>
−
<p>"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."</p>
+
<div class="line_divider"></div>
−
</div>
+
<div class="clear extra_space"></div>
  
−
<div class="clear"></div>
 
−
<br><br>
 
  
 +
<!--------------------------------------------------------------------------------------------- Question 14 --------------------------------------------------------------------------------------------------------->
  
−
<textarea rows="6" name="How your project works" data-form-field="how_it_works"></textarea>
 
  
  
−
<!-------------------------------------------------------------------------------------------- Question 12  --------------------------------------------------------------------------------------------------------->
 
  
 +
<p class="question">14. What rules or guidance cover your work which could help to manage any of the risks you identified in the last section?</p>
 +
<p class="note">
 +
For example: In your country / region, what are the laws and regulations that govern biosafety or biosecurity in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
 +
What are the guidelines for laboratory biosafety and biosecurity? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
 +
</p>
  
−
<bR><br><br>
 
−
<h3> Future applications  </h3>
 
  
 +
<textarea rows="5" name="rules_guidance_cover_your_work" data-form-field="rules_guidance_that_cover_work"></textarea>
  
−
<p class="question">12. How would your project be used in the real world?</p>
 
  
−
<p>Imagine that your project was fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes and expand in the comments section. (Note: iGEM teams <strong>should not release</strong> modified organisms into the natural environment.)
+
 
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
 +
 
 +
 
 +
 
 +
<!--------------------------------------------------------------------------------------------- Question 15 --------------------------------------------------------------------------------------------------------->
 +
 
 +
<p class="question">15. Have your team members received any safety training?</p>
 +
<p class="note">
 +
For the purposes of iGEM, biosafety and biosecurity training covers the procedures and practices used to manage risks from accidents or deliberate misuse of your projects to your team, colleagues, communities and the environment. All team members are expected to be aware of these risks and to work to manage them.
 
</p>
 
</p>
  
 
<ul>
 
<ul>
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_foundational"> Our project is foundational / we do not have a specific real-world application in mind </label><br> (Examples: library of standardized promoters, system for communication between cells)
+
<li><label><input type="checkbox" name="safety_training" data-form-field="safety_training_already_received" value="already_received_safety_training"> Yes, we have already received safety training.  </label></li>
−
</li>
+
 +
 +
<li><label><input type="checkbox" name="safety_training" data-form-field="security_training_already_received" value="already_received_security_training"> Yes, we have already received security training.  </label></li>
 +
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_lab"> Only in the lab</label><br />
+
−
(Examples: reporter strain for measuring the strength of promoters)</li>
+
<li><label><input type="checkbox" name="safety_training" data-form-field="safety_training_future" value="future_training"> We plan to receive safety and security training in the future. Please specify approximately when:  </label>
 +
<textarea rows="1" name="describe future training" data-form-field="safety_training_future_describe" data-validation="[data-form-field=safety_training_future]" placeholder="Please comment"></textarea></li>
 +
 +
 +
<li><label><input type="checkbox" name="safety_training"  data-form-field="safety_training_none" value="no_training"> We will not have safety and security training. Please explain in detail how team members will be aware of and manage risks to the team, colleagues, communities, and the environment in the absence of training. If it is not relevant because there is no lab component to your project, please note this:  </label>
 +
<textarea rows="1" name="describe no training" data-form-field="safety_training_none_describe" data-validation="[data-form-field=safety_training_none]" placeholder="Please comment"></textarea></li>
 +
 +
</ul>
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_factory"> In a factory</label><br />
 
−
(Examples: cells that make a flavor chemical for food, cells that make biofuel)
 
−
</li>
 
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_consumer"> In a consumer product that ordinary people buy</label><br />
 
−
(Examples: cells that clean your clothes, bread made with engineered yeast)
 
−
</li>
 
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_agriculture"> In agriculture / on a farm</label><br />
 
−
(Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)</li>
 
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_device"> In a small enclosed device</label><br />
+
<div class="clear extra_space"></div>
−
(Examples: a bio-sensing strip with cells that detect arsenic)
+
<div class="line_divider"></div>
−
</li>
+
<div class="clear extra_space"></div>
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_environment"> In the natural environment</label><br />
 
−
(Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought) </li>
 
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_humanbody"> To be used in the human body, or in food</label><br />
 
−
(Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants) </li>
 
  
−
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_other"> Other</label><br />
+
<!--------------------------------------------------------------------------------------------- Question 16 --------------------------------------------------------------------------------------------------------->
−
(Examples: bacteria that live on Mars) <textarea rows="1" name="Real world application other" data-form-field="real_world_application_other" data-validation="[data-form-field=rwa_other]" placeholder="Please describe"></textarea></li>
+
  
  
 +
<p class="question">16. Please select the topics that you learned about (or will learn about) in your safety training.</p>
 +
 +
<ul>
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_lab_rules" value="lab_access_and_rules"> Lab access and rules (including appropriate clothing, eating and drinking, etc.) </label></li>
 +
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_responsible_individuals" value="responsible_individuals">Responsible individuals (such as lab or departmental specialist or institutional biosafety officer) </label></li>
 +
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_biosafety_levels" value="biosafety_levels">Differences between biosafety levels</label></li>
 +
 
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_biosafety_equipment" value="biosafety_equipment">Biosafety equipment (such as biosafety cabinets)</label></li>
 +
 
 +
  <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_microbial_technique" value="microbial_technique">Good microbial technique (such as lab practices)</label></li>
 +
 
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_disinfection_sterilization" value="disinfection_sterilization">Disinfection and sterilization</label></li>
 +
 
 +
  <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_emergency_procedures" value="emergency_procedures">Emergency procedures</label></li>
 +
 
 +
    <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_transport_rules" value="transport_rules">Transport rules</label></li>
 +
 
 +
    <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_physical_biosecurity" value="physical_biosecurity">Physical biosecurity</label></li>
 +
 
 +
    <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_personnel_biosecurity" value="personnel_biosecurity">Personnel biosecurity</label></li>
 +
   
 +
  <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_dual_use_experiments" value="dual_use_experiments">Dual-use and experiments of concern</label></li>
 +
 
 +
<li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_data_biosecurity" value="data_biosecurity">Data biosecurity</label></li>
 +
 
 +
  <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_chemical_fire_electrical_safety" value="chemical_fire_electrical_safety">Chemicals, fire and electrical safety </label></li>
 +
 
 +
    <li><label><input type="checkbox" name="safety_training_learn" data-form-field="safety_learning_no_training" value="no_safety_training">We will not have safety training</label></li>
 +
 
 
</ul>
 
</ul>
  
  
−
<!-------------------------------------------------------------------------------------------- Question 13  --------------------------------------------------------------------------------------------------------->
 
  
−
<br><br>
+
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
  
−
<p class="question">13. What safety or ethical risks would be involved with such a use? </p>
 
  
−
<textarea rows="3" name="13. what are the safety ethical risks" data-form-field="safety_ethical_risks"></textarea>
+
<!--------------------------------------------------------------------------------------------- Question 17 --------------------------------------------------------------------------------------------------------->
  
 +
<p class="question">17. Which work areas do you use / are you using to handle biological materials? </p>
  
 +
<p class="note">
 +
Please check all the containment provisions you are using. If you are using more than one space please check all that apply and note this in the other work area box.
 +
</p>
  
  
 +
<ul>
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_no_lab" value="no_lab_work">No lab work (e.g. software project) </label></li>
 +
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_open_bench" value="open_bench">Open bench </label></li>
 +
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_biosafety_cabinet" value="biosafety_cabinet">Biosafety cabinet (please note there are <a href="https://ehs.umass.edu/sites/default/files/Fact%20Sheet%207%20BSC%20vs%20LFH_0.pdf"> important differences</a> between biosafety cabinets and laminar flow hoods / clean benches. iGEM encourages the use of biosafety cabinets but discourages the use of laminar flow hoods or clean benches) </label></li>
  
−
<!-------------------------------------------------------------------------------------------- Question 14 --------------------------------------------------------------------------------------------------------->
+
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_greenhouse" value="greenhouse">Specialist greenhouse </label></li>
−
<br><bR><br>
+
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_animal_house" value="animal_house">Specialist animal house </label></li>
 +
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_insect_facility" value="insect_facility">Specialist insect facility </label></li>
 +
 +
<li><label><input type="checkbox" name="work_areas"  data-form-field="work_areas_other" value="other">Other work area. Please describe:  </label>
 +
<textarea rows="1" name="describe other work area" data-form-field="other_work_area_describe" data-validation="[data-form-field=work_areas_other]" placeholder="Please comment"></textarea></li>
 +
 +
 +
<li><label><input type="checkbox" name="work_areas" data-form-field="work_areas_unknown" value="unknown">Unknown. Please comment:  </label>
 +
<textarea rows="1" name="describe unknown work area" data-form-field="unknown_work_area_describe" data-validation="[data-form-field=work_areas_unknown]" placeholder="Please comment"></textarea></li>
 +
 +
 +
</ul>
  
−
<h3> Specific risks </h3>
 
  
−
<p class="question">14.  Does your project include the use of gene drives? </p>
 
  
−
<div class="column half_size">
 
−
<ul>
 
  
−
<li><label><input type="radio"  data-form-field="gene_drives" value="gene_drive_no" name="gene_drives"> No </label></li>
 
  
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
  
−
<li><label><input type="radio"  data-form-field="gene_drives" value="gene_drive_yes"  name="gene_drives"> Yes </label> <br>
 
−
<font color="red"><b>STOP:</b></font> Gene Drives are not allowed in iGEM projects without a special exception from the Safety Committee. For more information see the <a href="https://2018.igem.org/Safety/Policies">Safety Policy </a>page and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. Please contact the Safety Committee by emailing safety AT igem DOT org
 
−
</li>
 
  
  
−
</ul>
+
<!--------------------------------------------------------------------------------------------- Question 18 --------------------------------------------------------------------------------------------------------->
−
</div>
+
  
  
−
<div class="column half_size message_box">
+
<p class="question">18. What is the biosafety Level of your lab?  </p>
−
<h4> Note </h4>
+
−
<p>For the purposes of iGEM, a gene drive includes Cas9 (and other endonucleases, such as dCas9 and Cpf1) integrated into the genome (including through the use of gRNA) of a sexually reproducing eukaryotic organisms (including organisms that reproduce both sexually and asexually, such as yeast) and/or the use of a drive to impact the progeny.
+
  
 +
<p class="note">
 +
<a href="https://2018.igem.org/Safety/Risk_Groups"> Help about Risk Groups and Safety Levels</a>
 
</p>
 
</p>
−
</div>
 
  
−
<div class="clear"></div>
+
<ul>
−
<br><br>
+
<li><label><input type="radio" name="biosafety_level" data-form-field="biosafety_level" value="not_applicable">Not applicable as we have no lab component </label></li>
−
<!-------------------------------------------------------------------------------------------- Question 15  --------------------------------------------------------------------------------------------------------->
+
 +
<li><label><input type="radio" name="biosafety_level" data-form-field="biosafety_level" value="level_one"> Level 1 (low risk)</label></li>
 +
 +
<li><label><input type="radio" name="biosafety_level" data-form-field="biosafety_level" value="level_two"> Level 2 (moderate risk)</label></li>
  
−
<p class="question">15. Does your project include the use of animals? </p>
+
<li><label><input type="radio" name="biosafety_level" data-form-field="biosafety_level" value="level_three"> Level 3 (high risk)</label></li>
 +
 +
<li><label><input type="radio" name="biosafety_level" data-form-field="biosafety_level" value="level_four"> Level 4 (extreme risk)</label></li>
 +
 +
<li><label><input type="radio" name="biosafety_level"  data-form-field="biosafety_level" value="level_other"> Other biosafety level. Please describe:    </label>
 +
<textarea rows="1" name="describe other biosafety level" data-form-field="other_biosafety_level_describe" data-validation="[value=level_other]" placeholder="Please comment"></textarea></li>
 +
 +
<li><label><input type="radio" name="biosafety_level"  data-form-field="biosafety_level" value="level_several"> We have several different lab spaces with different biosafety Levels. Please describe:    </label>
 +
<textarea rows="1" name="describe several biosafety level" data-form-field="several_biosafety_level_describe" data-validation="[value=level_several]" placeholder="Please comment"></textarea></li>
 +
 +
</ul>
  
 +
<p class="note">
 +
Note: iGEM teams should not use Risk Group 3 or 4 organisms, and they should not work in Safety Level 3 or 4 labs. If you are planning to work at Safety Level 3 or 4, contact safety (AT) igem (DOT) org right away!!
 +
</p>
  
−
<ul>
 
  
−
<li><label><input type="radio" data-form-field="animal_use" value="animal_use_no" name="animal_use">  No </label></li>
 
−
<li><label><input type="radio" data-form-field="animal_use" value="animal_use_yes" name="animal_use"> Yes </label> <br>
 
−
<font color="red"><b>STOP:</b></font> The use of animals is not allowed in iGEM projects without a special exception from the Safety Committee. For more information see the <a href="https://2018.igem.org/Safety/Policies">Safety Policy</a> page and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. Please contact the Safety Committee by emailing safety AT igem DOT org
 
−
</li>
 
  
  
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
  
−
</ul>
 
  
  
 +
<!--------------------------------------------------------------------------------------------- Question 19 --------------------------------------------------------------------------------------------------------->
  
  
−
<!-------------------------------------------------------------------------------------------- Question 16  --------------------------------------------------------------------------------------------------------->
 
  
−
<br><br>
+
<p class="question">19. How will the rules, training, containment and other procedures and practices help to manage any of the risks you identified in the last section?</p>
 +
<p class="note">
 +
Please give details of any steps you have taken to manage any risks identified. This might include things you deliberately didn't do. For example, if you are not conducting any experiments, especially on grounds of safety, security or as a responsible scientist / engineer, please note this. Examples might include, making sure you only use non-pathogenic strains of an organism, deciding that animal use experiments are not yet warranted, or avoiding plant infection experiments because the affected plant is found in your country.<br><br>
  
−
<p class="question">16. Does your project include the use of anti-microbial resistance factors? For more information see the <a href="https://2018.igem.org/Safety/Policies">Safety Policy </a>page and the <a href="https://2018.igem.org/Safety/White_List">White List.</a> </p>
+
Please also consider waste treatment – how will you know that any waste produced in your project will be successfully activated?  
 +
</p>
  
−
<div class="column half_size">
 
  
 +
<textarea rows="5" name="how will rules training containment and other manage risks" data-form-field="how_will_rules_training_help_manage_risks"></textarea>
  
  
−
<ul>
 
  
−
<li> <label>
+
</div>
−
<input type="radio"  data-form-field="antimicrobial_resistance" value="antimicrobial_resistance_no"  name="antimicrobial_resistance">No </label></li>
+
  
 +
</fieldset>
  
  
−
<li> <label>
 
−
<input type="radio"  data-form-field="antimicrobial_resistance" value="antimicrobial_resistance_yes_common"  name="antimicrobial_resistance"> Yes – the use of the resistance factor is common in research </label>
 
  
−
<p> <small> If you cannot provide such a reference you will need to complete a <a href="https://2018.igem.org/Safety/Check_In">check-in form</a> for this part. </small></p>
 
  
−
<textarea rows="1" name="common_resistance_descrip" data-form-field="common_resistance_descrip" data-validation="[value=antimicrobial_resistance_yes_common]" placeholder="Please provide a reference"></textarea>
 
  
−
</li>
+
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
  
−
<li> <label>
+
Part 3 - Rules
−
<input type="radio"  data-form-field="antimicrobial_resistance"  value="antimicrobial_resistance_yes_connected"  name="antimicrobial_resistance"> Yes – the resistance factor is connected to an antimicrobial in clinical use </label>
+
  
−
<p> <small>You will need to complete a <a href="https://2018.igem.org/Safety/Check_In">check-in form</a> for this part. </small></p>
+
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
−
<textarea rows="1" name="real_world_descrip" data-form-field="antimicrobial_resistance_descrip" data-validation = "[value=antimicrobial_resistance_yes_connected]" placeholder="Please provide a reference"></textarea>
+
  
−
</li>
+
<fieldset>
 +
<div class="column full_size">
  
 +
<h2>3. Compliance with iGEM’s rules and policies</h2>
  
−
<li> <label>
 
−
<input type="radio"  data-form-field="antimicrobial_resistance" value="antimicrobial_resistance_yes_another"  name="antimicrobial_resistance"> Yes – it is another resistance factor </label>
 
−
<textarea rows="1" name="antimicrobial_other_descrip" data-form-field="antimicrobial_other_descrip" data-validation="[value=antimicrobial_resistance_yes_another]" placeholder="Please provide details"></textarea>
 
−
</li>
 
  
  
−
</ul>
+
<!--------------------------------------------------------------------------------------------- Question 20 --------------------------------------------------------------------------------------------------------->
−
</div>
+
  
  
  
−
<div class="column half_size message_box">
+
<p class="question">20. Are you planning / have released any organism or product derived from your project?  </p>
−
<h4> Note </h4>
+
 
−
<p> For the purposes of iGEM, anti-microbial resistance factors are genetic sequences, or any other factor, that can increase an organism's resistance to drugs or therapeutics used to control them. This includes genes known to confer resistance to antibiotics or antiviral drugs.
+
<p class="note">
 +
For the purposes of iGEM, release includes putting any engineered organism or product from one into the environment, yourselves or volunteers (including by eating or drinking), or into a device that will be placed in the environment.
 
</p>
 
</p>
−
</div>
 
  
 +
<ul>
 +
<li><label><input type="radio" name="release_organism_or_product" data-form-field="release_organism_or_product" value="no_releasing">No </label></li>
 +
 +
<li><label><input type="radio" name="release_organism_or_product" data-form-field="release_organism_or_product" value="yes_releasing">Yes <br>  <span class="red_text">STOP: </span> Release is not allowed in iGEM. For more information see the <a href="https://2018.igem.org/Safety/Policies">policy page</a>. Please contact the Safety and Security Committee by emailing safety AT igem DOT org</label></li>
  
 +
</ul>
  
  
 +
<div class="clear extra_space"></div>
 +
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
  
  
−
<div class="clear"></div>
 
  
−
<!-------------------------------------------------------------------------------------------- Question 17  --------------------------------------------------------------------------------------------------------->
+
<!--------------------------------------------------------------------------------------------- Question 21 --------------------------------------------------------------------------------------------------------->
  
−
<br><br><br>
 
−
<h3>Parts and organisms not on the Whitelist </h3>
 
  
  
 +
<p class="question">21. Are you planning  to make / or have made a gene drives?</p>
 +
<p class="note">For the purposes of iGEM, a gene drive includes Cas9 (and other endonucleases, such as dCas9 and Cpf1) integrated into the genome (including through the use of gRNA) of a sexually reproducing eukaryotic organisms (including organisms that reproduce both sexually and asexually, such as yeast) and/or the use of a drive to impact the progeny.</p>
  
−
<p class="question">17. Does your project include parts or organisms not on the <a href="https://2018.igem.org/Safety/White_List">Whitelist.</a>?</p>
 
  
−
 
−
<div class="column half_size">
 
 
<ul>
 
<ul>
 +
<li><label><input type="radio" name="gene_drives" data-form-field="gene_drives" value="gene_drives_no">No </label></li>
 +
 +
<li><label><input type="radio" name="gene_drives" data-form-field="gene_drives" value="gene_drives_yes">Yes <br>  <span class="red_text">STOP: </span> Gene Drives are not allowed in iGEM projects without a special exception from the Safety and Security Committee. For more information see the <a href="https://2018.igem.org/Safety/Policies">policy page</a> and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. Please contact the Safety and Security Committee by emailing safety AT igem DOT org</label></li>
  
−
<li><label><input type="radio"  data-form-field="white_list" value="white_list_no" name="white_list"> No </label></li>
+
</ul>
  
  
−
<li><label><input type="radio"  data-form-field="white_list" value="white_list_yes" name="white_list"> Yes </label> <br>
 
−
<font color="red"><b>STOP:</b></font> Before you acquire or use any organism/part that is NOT on the Whitelist, you must submit a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a>. Check-Ins allow the iGEM Safety Committee to help you ensure that you will work safely with these riskier organisms/parts. The Safety Committee will base its review on the information you provide – please provide as much information as possible and use references as appropriate.
 
  
−
</li>
 
  
  
  
−
</ul>
+
<div class="clear extra_space"></div>
−
</div>
+
<div class="line_divider"></div>
 +
<div class="clear extra_space"></div>
  
  
  
−
<div class="column half_size message_box">
 
−
<h4> Note </h4>
 
−
<p> Some organisms and parts present risks beyond what is ordinary for lab work in synthetic biology. As your project progresses, you should consider the risks presented by each organism and part you plan to use.</p>
 
−
</div>
 
  
  
−
<div class="clear"></div>
+
<!--------------------------------------------------------------------------------------------- Question 22 --------------------------------------------------------------------------------------------------------->
−
<!-------------------------------------------------------------------------------------------- Question 18  --------------------------------------------------------------------------------------------------------->
+
  
−
<br><br><br>
 
−
<h3>Chassis</h3>
 
  
−
<p class="question">18. What is your chassis organism?</p>
 
−
<p>Check all species you are genetically modifying in your project.</p>
 
  
 +
<p class="question">22. Are you planning / have used resistance factors (or associated sequences) for critically or highly clinically important antimicrobials, as listed by the World Health Organization? </p>
 +
<p class="note">For the current list of these antimicrobials see the table on p10 of this <a href="http://apps.who.int/iris/bitstream/handle/10665/255027/9789241512220-eng.pdf;jsessionid=F724126C3309FAAD629FB7D9DE9E5A9A?sequence=1">report</a>. You need NOT include any resistance factors included in iGEM’s distribution kit.</p>
  
  
−
<div class="column half_size">
 
 
<ul>
 
<ul>
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_yeast"> Yeast (Saccharomyces)</label></li>
+
<li><label><input type="radio" name="resistance_factors" data-form-field="resistance_factors" value="resistance_factors_no">No </label></li>
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_lactobacillus"> Lactobacillus</label></li>
+
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_bsubtilis"> B. subtilis</label></li>
+
<li><label><input type="radio" name="resistance_factors" data-form-field="resistance_factors" value="resistance_factors_yes">Yes <br> <span class="red_text">STOP: </span> Before you acquire or use resistance factors and associated sequences for critically or highly clinically important antimicrobials, you must - submit a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a>. Check-Ins allow the iGEM Safety and Security Committee to help you ensure that you will work safely with these riskier organisms/parts. The Safety and Security Committee will base its review on the information you provide – please provide as much information as possible and use references as appropriate.</label></li>
 +
 
 
</ul>
 
</ul>
  
  
−
</div>
+
 
−
<div class="column half_size message_box">
+
 
−
<h4> Note </h4>
+
<div class="clear extra_space"></div>
−
<p> For the purposes of iGEM, a chassis is the organism in which you are putting your parts, or which you are modifying in your project. Many teams will use a common lab organism as a chassis. Some teams may use a more exotic organism. Some project may not involve a chassis.
+
<div class="line_divider"></div>
−
</p>
+
<div class="clear extra_space"></div>
−
</div>
+
 
 +
 
 +
<!--------------------------------------------------------------------------------------------- Question 23 --------------------------------------------------------------------------------------------------------->
 +
 
 +
 
 +
<p class="question">23. Are you planning to use, or using any animals (including insects and invertebrates) not on the <a href="https://2018.igem.org/Safety/White_List">Whitelist?</a></p>
 +
 
 
<ul>
 
<ul>
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_ecoli"> E. coli (give names of all strains you are using):</label> <textarea rows="2" name="E. coli strain" data-form-field="chassis_ecoli_describe" data-validation="[data-form-field=chassis_ecoli]"></textarea></li>
+
<li><label><input type="radio" name="using_animals" data-form-field="using_animals" value="using_animals_no">No </label></li>
 +
 +
<li><label><input type="radio" name="using_animals" data-form-field="using_animals" value="using_animals_yes">Yes <br> 
 +
<span class="red_text">STOP: </span>Before you acquire or use any animal NOT on the <a href="https://2018.igem.org/Safety/White_List">Whitelist</a>, you must - submit a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a>. Check-Ins allow the iGEM Safety and Security Committee to help you ensure that you will work safely and responsible with these organisms. The Safety and Security Committee will base its review on the information you provide – please provide as much information as possible and use references as appropriate.</label></li>
  
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_other"> Others (give species names): </label> <textarea rows="2" name="Other chassis (give species names)" data-form-field="chassis_other_describe" data-validation="[data-form-field=chassis_other]"></textarea></li>
 
−
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_none"> No chassis organism (please comment):</label> <textarea rows="2" name="No chassis (comment)" data-form-field="chassis_none_describe" data-validation="[data-form-field=chassis_none]"></textarea></li>
 
 
</ul>
 
</ul>
  
−
<p class="question">Comments:</p>
 
−
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<h3>Other organisms (or parts from them)</h3>
+
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<p class="question">19. Besides your chassis, do you plan to use any other organisms (or parts from them)?</p>
 
  
  
−
<p>What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains. <strong>This includes the origins of parts taken from other organisms</strong>, even if they are being synthesized rather than isolated from nature – you need not include any parts already in the registry.</p>  
+
<!--------------------------------------------------------------------------------------------- Question 24 --------------------------------------------------------------------------------------------------------->
 +
 
 +
 
 +
 
 +
<p class="question">24. Are you planning to use / have used any vertebrates (e.g. rats, mice, guinea pigs, hamsters) or higher order invertebrates (e.g. cuttlefish, octopus, squid, lobster)?</p>
  
−
<br><br>
 
−
<h5>Example answers:</h5>
 
 
<ul>
 
<ul>
−
          <li>"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."</li>
+
<li><label><input type="radio" name="using_vertebrates" data-form-field="using_vertebrates" value="using_vertebrates_no">No </label></li>
−
          <li>"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."</li>
+
−
          <li>"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."</li>
+
<li><label><input type="radio" name="using_vertebrates" data-form-field="using_vertebrates" value="using_vertebrates_yes">Yes <br>  <span class="red_text">STOP: </span> Before you acquire or use any vertebrate you must submit an <a href="https://2018.igem.org/Safety/Animal_Use_Check_In">Animal Use form</a>. The use of animals is not allowed in iGEM projects without a special exception from the Safety and Security Committee. For more information see the <a href="https://2018.igem.org/Safety/Policies">policy page</a> and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. Please contact the Safety and Security Committee by emailing safety AT igem DOT org </label></li>
−
          <li>“We are interested in a RNA-binding protein expressed in Kluyveromyces lactis. We have found the sequence in a paper and will have is synthesized by a provider.” </li>
+
−
</ul></p>
+
  
−
<br><br>
+
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−
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−
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+
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−
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+
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−
<h3>Uncertainties</h3>
+
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−
<p class="question">20. Are there parts of your project which you think may have ethical, safety or security concerns that are not fully covered by current rules and standards?</p>
 
  
−
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−
<!-------------------------------------------------------------------------------------------- Question 20  --------------------------------------------------------------------------------------------------------->
+
<!--------------------------------------------------------------------------------------------- Question 25 --------------------------------------------------------------------------------------------------------->
  
−
<br><br>
 
  
−
<p class="question">21. Who have you worked with to resolve any uncertainties or gaps in how you ensure the safety of your project and how difficult have they been to contact?</p>
+
<p class="question">25. Are you planning to use, or using any parts not on the <a href="https://2018.igem.org/Safety/White_List">Whitelist?</a></p>
  
−
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+
<ul>
 +
<li><label><input type="radio" name="using_parts_not_in_whitelist" data-form-field="using_parts_not_in_whitelist" value="using_parts_not_in_whitelist_no">No </label></li>
 +
 +
<li><label><input type="radio" name="using_parts_not_in_whitelist" data-form-field="using_parts_not_in_whitelist" value="using_parts_not_in_whitelist_yes">Yes <br>  <span class="red_text">STOP: </span> Before you acquire or use any part that is NOT on the Whitelist, you must - submit a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a>. Check-Ins allow the iGEM Safety and Security Committee to help you ensure that you will work safely with these riskier parts. The Safety and Security Committee will base its review on the information you provide – please provide as much information as possible and use references as appropriate. </label></li>
  
−
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+
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−
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−
<h2> Note: </h2>
+
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+
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 +
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 +
<p class="question">26. Are you planning to use, or using any parts or organisms obtained from outside the lab or regular suppliers?</p>
  
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<ul>
 +
<li><label><input type="radio" name="using_parts_organisms_from_outside" data-form-field="using_parts_organisms_from_outside" value="using_parts_organisms_from_outside_no">No </label></li>
 +
 +
<li><label><input type="radio" name="using_parts_organisms_from_outside" data-form-field="using_parts_organisms_from_outside" value="using_parts_organisms_from_outside_yes">Yes <br>  <span class="red_text">STOP: </span>Before you acquire or use any organism/part that has come from outside the lab or regular suppliers, you must - submit a <a href="https://2018.igem.org/Safety/Check_In">Check-In form</a>. Check-Ins allow the iGEM Safety and Security Committee to help you ensure that you will work safely with these riskier organisms/parts. The Safety and Security Committee will base its review on the information you provide – please provide as much information as possible and use references as appropriate.</label></li>
  
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<h2> 4.- New Parts </h2>
 
−
<p> This part of the of the form is for you to tell us about the parts you have developed during your project. It summarises information that might already have been submitted through check-in forms.
 
−
Please visit this <a href="https://2018.igem.org/File:TeamName_Safety2018_Spreadsheet.xlsx">page</a> to download a blank copy of the spreadsheet for this question. (If you need a CSV version instead of XLSX, visit this <a href="https://2018.igem.org/File:TeamName_Safety2018_Spreadsheet.csv">page</a>.)
 
−
Complete the spreadsheet. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.
 
−
You may omit non-protein-coding parts (except if they are known virulence factors – you should undertake a literature search to determine if they are), and you may omit parts that were already in the Registry if you are using them without significant modifications. For more information on virulence factors see the <a href="https://2018.igem.org/Safety/Policies">Safety Policy</a> page and the <a href="https://2018.igem.org/Safety/White_List">White List</a>. Please contact the Safety Committee by emailing safety AT igem DOT org
 
−
</p>
 
  
  
 +
<!--------------------------------------------------------------------------------------------- Question 27 --------------------------------------------------------------------------------------------------------->
  
−
<DIV class="highlightBoxB">
+
<p class="question">27. What else can you tell us about any risks associated with your project, how you are managing them, or your compliance with iGEM’s safety and security <a href="https://2018.igem.org/Safety/Rules">rules</a> and <a href="https://2018.igem.org/Safety/Policies">policies?</a> </p>
−
    <a href="#" class="click_expand">Click here to show/hide instructions for completing the spreadsheet</a>
+
<p class="note">This can include any improvements you would like to see to our safety and security efforts, or anything that has not been sufficiently clear, or where additional guidance would be useful, or where you see important uncertainties.</p>
−
    <div>
+
−
        <p><strong>Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".</strong></p>
+
−
        <ol style="list-style-type:upper-alpha">
+
−
            <li><strong>Species name (including strain):</strong> For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.</li>
+
−
            <li><strong>Risk Group:</strong> Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)</li>
+
−
            <li><strong>Risk Group Source:</strong> Cite the source from which you obtained the Risk Group information. See <a target="_blank" href="https://2018.igem.org/Safety/Risk_Groups#HowToFindRiskGroup">Risk Group Guide</a> for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.</li>
+
−
            <li><strong>Disease risk to humans?:</strong> Does this organism cause any disease in humans? If yes, what disease does it cause?</li>
+
−
            <li><strong>Part number/name:</strong> For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.</li>
+
−
            <li><strong>Natural function of part:</strong> For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.</li>
+
−
            <li><strong>How did you acquire it?:</strong> Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)</li>
+
−
            <li><strong>How will you use it?:</strong> Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")</li>
+
−
            <li><strong>Notes:</strong> Use this column to give any additional information that is necessary.</li>
+
−
        </ol>
+
−
    </div>
+
−
</DIV>
+
−
<br />
+
  
−
<p><strong><a href="" target="_blank" id="spreadsheet_upload"><button type="button">Upload Spreadsheet</button></a> -- Please do not change the "Destination Filename"!</strong> [File:<em>TeamName</em> Safety2018 Spreadsheet.xls]</p>
 
−
<p>You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.</p>
 
−
<p><strong><a href="" target="_blank" id="spreadsheet_view">Click here to VIEW your spreadsheet</a></strong></p>
 
−
<br />
 
−
</fieldset>
 
  
 +
<textarea rows="5" name="more_info_managing_risks" data-form-field="more_info_managing_risks"></textarea>
  
  
  
 +
 +
<div class="highlight">
 +
<p>The information above will save automatically. Please fill as much information as possible by <b> June 29, 2018</b> </p>
 +
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 +
 +
 +
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 +
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 +
  
  
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−
SUBMIT
+
Sign Off
  
 
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 +
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−
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−
<h2>5.- Sign Off</h2>
 
  
 +
<div class="column full_size">
  
 +
<h2>Sign off </h2>
  
−
<p>Only a team Instructor or PI may submit the Safety Form.</p>
+
<h3>Due September 7, 2018</h3>
−
<p>Instructors/PIs, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Final Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.</p>
+
<p class="note">Only a team Instructor or PI may submit the Safety and Security Form.
 +
Instructors/PIs, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety and Security Form accurately describes the activities of your team and that they have complied with all relevant institutional, national or regional laws, rules and regulations.  
 +
We are using the "I Agree" box in lieu of a signature with paper and pen.
 +
</p>
  
 +
<label id="sign_off_button">  <input type="checkbox" name="I_Agree" data-form-field="submit" value="agree"  id="i_agree">  I Agree </label>
  
 +
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 +
<label id="submitted_agree"> <input type="checkbox" checked disabled>  I Agree ✓ </label>
  
  
−
<label> <input type="checkbox" name="I_Agree" data-form-field="submit" value="agree"  id="i_agree">  I Agree </label>
 
  
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 +
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Latest revision as of 14:11, 12 December 2018

Loading...

Safety Form

The purpose of this form is for everyone, including iGEM, to know you are doing your project safely and securely. This is how we know that everyone in iGEM is assessing and managing any risks to yourselves, your colleagues, communities or the environment. If you don't tell us how you are dealing with these risks, we don't know. Being transparent about any possible risks and how we are managing them is a key component of being a responsible scientist or engineer - so please answer the questions as fully as possible.

Important

Complying with the safety and security requirements is a condition of competing at iGEM - that includes providing all the information in this form by the relevant deadlines. Failure to do so can lead to immediate disqualification from the competition and referral to the Responsible Conduct Committee.

Deadlines

The form should be updated throughout the project whenever there is a change that alters possible risks or the procedures, practices and containment provisions being used to manage them.

June 28th

An initial version of this form, with as much information as possible, is to be provided by the team by June 28th. The form should NOT be signed off by the PI.

September 13th

A final version of this form must be signed off by the PI by September 13th.
(Please note, that you will need to plan ahead - your PI being unavailable or on vacation is not an acceptable excuse for late submission of this form)

Approach

In general, we are looking for information in three areas. We want you to explain:

  1. What risks might be connected to your project now or in the future (Part 1);
  2. What procedures, practices and containment provisions you are using to manage the risks you identify (Part 2); and
  3. That you are complying with iGEM’s safety and security rules and policies (Part 3).

Other required safety and security information

Any time you are planning to use an organism or part not on the White List the team needs to submit a Check-in Form before using them.

Any time you are planning to use a vertebrate animal in your project the team needs to submit an Animal Use Form before using them.

Safety Form

Team Selection

Please choose a team

Overview

Contact Information

Name

Email

1. Please upload a photo or two of your lab to the iGEM 2018 server (include your team name in the file name), preferably showing the relevant safety features and paste the link here:

(Instructions on how to upload an image to our servers can be found here)

2. Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts.

(Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)

Describe your project
This part of the form is for you to tell us about your primary project idea, what organisms and parts you will use and what your project will do. We know that iGEM teams often change project topics during the competition. When you change your primary project idea, please update this form to tell us about your new idea! If you are still working on multiple project ideas by June 29, you may choose one to describe on this form. If there are major changes to your project after June 29, please contact the Safety and Security Committee by emailing safety AT igem DOT org.

Good example answers
"Our bacteria will be engineered to interact with human cells. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."
"Our algae will receive gasses high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2 from the gas."

Bad example answers (not enough detail)
"We are engineering E. coli to cure liver cancer."
"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."

1. Identifying possible risks

iGEM has developed a Risk Assessment Tool to help you identify possible risks to you, your colleagues, communities, or the environment. We encourage you to use this tool before filling in this part of the form. What are you using / planning to use? Some organisms and parts present risks beyond what is ordinary for lab work in synthetic biology. As your project progresses, you should consider the risks presented by each organism and part you plan to use.

3. Which whole organisms, including viruses, are you planning to use or using in your project?

Please provide as much detail as possible (such as strain information). If you are not using an organism, please note this.

4. What risks could these organisms pose to you or your colleagues, or to your community or the environment if they escape the lab?

If you are not using an organism, please note this.

5. Check all species you are using as a chassis in your project.

What is your chassis organism?
For the purposes of iGEM, a chassis is the organism in which you are putting your parts, or which you are modifying in your project. Many teams will use a common lab organism as a chassis. Some teams may use a more exotic organism. Some project may not involve a chassis.

Comments:

6. What risks could your chassis pose to you or your colleagues, or to your community or the environment if they escape the lab?

If not using a chassis organism, please note this.

What parts are you making or planning to make?
This part of the of the form is for you to tell us about the parts you are planning to make or have developed during your project. It summarises information that might already have been submitted through Check-in forms. If you submitted a Check-In form for an organism or part, you should still include it in this section. You may omit non-protein-coding parts (except if they are known virulence or anti-microbial factors – you should undertake a literature search to determine if they are), and you may omit parts that were already in the Registry if you are using them without significant modifications. For more information on virulence factors see the Safety Policy page and the White List. If there are major changes to your project after June 29, please contact the Safety and Security Committee by emailing safety AT igem DOT org.

7. As part of your project, are you are planning to make / have made new parts or substantively changed existing parts in the Registry.

8. Part information is submitted in a spreadsheet.

Please visit this page to download a blank copy of the spreadsheet for this question. (If you need a CSV version instead of XLSX, visit this page.)

DOWNLOAD SPREADSHEET

Instructions for parts spreadsheet: Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".

A. Species name (including strain): For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.

B. Risk Group: Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)

C. Risk Group Source: Cite the source from which you obtained the Risk Group information. See Risk Group Guide for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.

D. Disease risk to humans: Does this organism cause any disease in humans? If yes, what disease does it cause?

E. Disease or other risks to the environment: Does the organism cause plant or animal diseases or would in any other way pose a risk to the environment if accidentally released?

F. Part number/name:For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.

G. Natural function of part: For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.

H.How did you acquire it: Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)

I. How will you use it: Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")

J. Notes: Use this column to give any additional information that is necessary.

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9. What experiments will you do with your organisms and parts?

Please explain briefly. We are particularly keen to understand the boundaries or scope of your project. You should include the names of species / cell lines / strains. You should include experiments involving parts taken from other organisms, even if they are being synthesized rather than isolated from nature – you need not include any parts already in the registry.

Example answers
"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."
"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."
"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."
“We are interested in a RNA-binding protein expressed in Kluyveromyces lactis. We have found the sequence in a paper and will have it synthesized by a provider.”

10. What risks could arise from these experiments?

For example, could they produce aerosols making it more likely that you could inhale something? Or are you using needles and could accidentally stick yourself? Could you produce something that is not inactivated using standard lab protocols? If you are not conducting any experiments, please note this.

11. Imagine that your project was fully developed into a real product that real people could use. How would people use it?

Check all appropriate boxes and expand in the comments section. (Note: iGEM teams should not release modified organisms into the natural environment but you could imagine such a release if your project was fully developed.)

12. What safety, security or ethical risks would be involved with such a use?

It is possible that software projects could also pose relevant risks.

2. Managing the risks you have identified

It is impossible to remove all risk from any biological activity. Instead, we attempt to manage these risks - to reduce them to an acceptable level. In this section, you should outline what steps have been taken (or you are planning to take) to reduce any risks you identified in the first part of the form.

13. How will experts overseeing your project help to manage any of the risks you identified in this form?

For example, who is responsible for the safety and security of biology labs at your institution? What role has any institutional biosafety officer played in reviewing your work? What skills do these experts have that might help? For example, do they have a long history of working with an organism or part that might pose a risk? How familiar are they with the experimental procedures and practices you will be, or are, using?

14. What rules or guidance cover your work which could help to manage any of the risks you identified in the last section?

For example: In your country / region, what are the laws and regulations that govern biosafety or biosecurity in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link. What are the guidelines for laboratory biosafety and biosecurity? Please give a link to these guidelines, or briefly describe them if you cannot give a link.

15. Have your team members received any safety training?

For the purposes of iGEM, biosafety and biosecurity training covers the procedures and practices used to manage risks from accidents or deliberate misuse of your projects to your team, colleagues, communities and the environment. All team members are expected to be aware of these risks and to work to manage them.

16. Please select the topics that you learned about (or will learn about) in your safety training.

17. Which work areas do you use / are you using to handle biological materials?

Please check all the containment provisions you are using. If you are using more than one space please check all that apply and note this in the other work area box.

18. What is the biosafety Level of your lab?

Help about Risk Groups and Safety Levels

Note: iGEM teams should not use Risk Group 3 or 4 organisms, and they should not work in Safety Level 3 or 4 labs. If you are planning to work at Safety Level 3 or 4, contact safety (AT) igem (DOT) org right away!!

19. How will the rules, training, containment and other procedures and practices help to manage any of the risks you identified in the last section?

Please give details of any steps you have taken to manage any risks identified. This might include things you deliberately didn't do. For example, if you are not conducting any experiments, especially on grounds of safety, security or as a responsible scientist / engineer, please note this. Examples might include, making sure you only use non-pathogenic strains of an organism, deciding that animal use experiments are not yet warranted, or avoiding plant infection experiments because the affected plant is found in your country.

Please also consider waste treatment – how will you know that any waste produced in your project will be successfully activated?

3. Compliance with iGEM’s rules and policies

20. Are you planning / have released any organism or product derived from your project?

For the purposes of iGEM, release includes putting any engineered organism or product from one into the environment, yourselves or volunteers (including by eating or drinking), or into a device that will be placed in the environment.

21. Are you planning to make / or have made a gene drives?

For the purposes of iGEM, a gene drive includes Cas9 (and other endonucleases, such as dCas9 and Cpf1) integrated into the genome (including through the use of gRNA) of a sexually reproducing eukaryotic organisms (including organisms that reproduce both sexually and asexually, such as yeast) and/or the use of a drive to impact the progeny.

22. Are you planning / have used resistance factors (or associated sequences) for critically or highly clinically important antimicrobials, as listed by the World Health Organization?

For the current list of these antimicrobials see the table on p10 of this report. You need NOT include any resistance factors included in iGEM’s distribution kit.

23. Are you planning to use, or using any animals (including insects and invertebrates) not on the Whitelist?

24. Are you planning to use / have used any vertebrates (e.g. rats, mice, guinea pigs, hamsters) or higher order invertebrates (e.g. cuttlefish, octopus, squid, lobster)?

25. Are you planning to use, or using any parts not on the Whitelist?

26. Are you planning to use, or using any parts or organisms obtained from outside the lab or regular suppliers?

27. What else can you tell us about any risks associated with your project, how you are managing them, or your compliance with iGEM’s safety and security rules and policies?

This can include any improvements you would like to see to our safety and security efforts, or anything that has not been sufficiently clear, or where additional guidance would be useful, or where you see important uncertainties.

The information above will save automatically. Please fill as much information as possible by June 29, 2018

Sign off

Due September 7, 2018

Only a team Instructor or PI may submit the Safety and Security Form. Instructors/PIs, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety and Security Form accurately describes the activities of your team and that they have complied with all relevant institutional, national or regional laws, rules and regulations. We are using the "I Agree" box in lieu of a signature with paper and pen.


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