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<div class = "date" id="71618"> 7/16/18 </div> | <div class = "date" id="71618"> 7/16/18 </div> | ||
<div class = "day" id="716"> We diluted primers for plasmid 1 to 100x and used those primers to amplify our plasmid 1 parts. We also made more chloramphenicol plates, transformed the plasmids for InterLab into competent NEB5-alpha cells, replated cells for InterLab onto chloramphenicol plates, and created a glycerol stock for our InterLab cells. | <div class = "day" id="716"> We diluted primers for plasmid 1 to 100x and used those primers to amplify our plasmid 1 parts. We also made more chloramphenicol plates, transformed the plasmids for InterLab into competent NEB5-alpha cells, replated cells for InterLab onto chloramphenicol plates, and created a glycerol stock for our InterLab cells. | ||
− | <br> Members: Brittany and Elisa </div> | + | <br> Members: Brittany and Elisa <br> We ran parts 1-4 and 1-5 in a 2% gel and extracted part 1-4 (1-5 was not amplified.) |
+ | We also designed new primers for the extraction and submission of parts that will allow us to put our parts in the pSB1C3 backbone for submission to the registry. | ||
+ | <br> Members: Elisa | ||
+ | </div> | ||
<div class="date" id="71718"> 7/17/18 </div> | <div class="date" id="71718"> 7/17/18 </div> | ||
<div class = "day" id="717"> We inoculated cultures of transformed InterLab cells. We performed the InterLab Absorbance Calibration measured at 630 nm. We also performed the InterLab microsphere calibration measured at 630 nm. Finally, we transformed the part from well L4 into NEB5-alpha cells. | <div class = "day" id="717"> We inoculated cultures of transformed InterLab cells. We performed the InterLab Absorbance Calibration measured at 630 nm. We also performed the InterLab microsphere calibration measured at 630 nm. Finally, we transformed the part from well L4 into NEB5-alpha cells. |
Revision as of 14:08, 18 July 2018