Line 191: | Line 191: | ||
$("#6718").click(function(){ | $("#6718").click(function(){ | ||
$("#67").slideToggle("slow"); | $("#67").slideToggle("slow"); | ||
+ | }); | ||
+ | $("#61918").click(function(){ | ||
+ | $("#619").slideToggle("slow"); | ||
+ | }); | ||
+ | $("#62118").click(function(){ | ||
+ | $("#621").slideToggle("slow"); | ||
+ | }); | ||
+ | $("#62518").click(function(){ | ||
+ | $("#625").slideToggle("slow"); | ||
+ | }); | ||
+ | $("#62618").click(function(){ | ||
+ | $("#626").slideToggle("slow"); | ||
+ | }); | ||
+ | $("#7618").click(function(){ | ||
+ | $("#76").slideToggle("slow"); | ||
}); | }); | ||
$("#71218").click(function(){ | $("#71218").click(function(){ | ||
Line 387: | Line 402: | ||
<div class = "day" id="66"> Our first day in lab! After some training earlier in the week, we began our work in the actual lab. We learned how to make different broths and agars, and made Luria broth (LB) plates with chloramphenicol and LB plates with ampicillin. <br> Members: Ariel, Brittany, Emilie, Liz, Lining, Kaylee, and Elisa <br> | <div class = "day" id="66"> Our first day in lab! After some training earlier in the week, we began our work in the actual lab. We learned how to make different broths and agars, and made Luria broth (LB) plates with chloramphenicol and LB plates with ampicillin. <br> Members: Ariel, Brittany, Emilie, Liz, Lining, Kaylee, and Elisa <br> | ||
<img src = "https://static.igem.org/mediawiki/2018/d/d3/T--RHIT--6618.jpg" style="width:30%; transform:rotate(90deg); margin-top:40px"></div> | <img src = "https://static.igem.org/mediawiki/2018/d/d3/T--RHIT--6618.jpg" style="width:30%; transform:rotate(90deg); margin-top:40px"></div> | ||
− | |||
− | |||
<div class = "week"> Week 2 </div> | <div class = "week"> Week 2 </div> | ||
+ | <div class = "date" id = "61218"> 6/12/18 </div> | ||
+ | <div class = "day" id="612"> We compiled information about possible part sequences to use in our project and ran them through the Parts Registry to see what we can and cannot submit as our own parts. We then created several different sequences of one of our main parts, PETase until we decided upon an optimized mutant version that was recently discovered in April 2018. We created some rough sketches of what our complete plasmid would look like. In lab, we created competent cells out of E. coli strains MG1655 and BL21(DE3).<br> Members: Elisa and Lining </div> | ||
<div class = "week"> Week 3 </div> | <div class = "week"> Week 3 </div> | ||
+ | <div class = "date" id="61918"> 6/19/18 </div> | ||
+ | <div class = "day" id="619"> We discovered that our parts were too large to fit on a single plasmid, so two were developed. One uses pSB1C3 as the backbone, and the other uses pSB3K5.We decided to have one plasmid have the PETase and MHETase enzyme sequences while the second plasmid would have sequences for glycolaldehyde dehydrogenase, glycolaldehyde reductase, glycolate oxidase, and malate synthase. <br> Members: Elisa and Lining </div> | ||
+ | <div class = "date" id="62118"> 6/21/18 </div> | ||
+ | <div class = "day" id="621"> We emailed the researchers about the optimised PETase and did more research on previous teams who had tried to solve the plastic problem the world faces today. We then transformed the plasmids into competent 5Alpha cells to culture, but discovered that pSB3K5 does not allow the cell to grow on the plate we wanted. <br> Members: Elisa and Lining </div> | ||
<div class = "week"> Week 4 </div> | <div class = "week"> Week 4 </div> | ||
+ | <div class = "date" id="62518"> 6/25/18 </div> | ||
+ | <div class = "day" id="625"> We decided to use pSB3K3 as the other backbone to replace pSB3K5. We then created linearized backbones by digestion. We started to develop primers so that we could amplify our parts once we received them. <br> Members: Elisa and Lining </div> | ||
+ | <div class = "day" id="62618"> 6/26/18 </div> | ||
+ | <div class = "day" id="626"> We sent out our part sequences to be synthesized through IDT, using our two plasmid ideas. The parts all had about 30 base pairs of overlap so we could put them together in the cell. We also designed our parts so there is a ribosomal binding site between the start of each enzyme, so they can be transcribed and translated into proteins individually if need be. <br> Members: Elisa and Lining</div> | ||
<div class = "week"> Week 5 </div> | <div class = "week"> Week 5 </div> | ||
+ | <div class = "date" id="7618"> 7/6/18 </div> | ||
+ | <div class = "day" id="76"> IDT was unable to synthesize all of our DNA sequences, so we had to break several of our sequences into smaller ones. Due to this, we had to create new primers for amplification of our new blocks. We also practiced running an SDS page, making sure that we had all the proper chemicals to do so. <br> Members: Elisa and Lining </div> | ||
<div class = "week"> Week 6 </div> | <div class = "week"> Week 6 </div> | ||
<div class = "date" id="71218"> 7/12/18 </div> | <div class = "date" id="71218"> 7/12/18 </div> |
Revision as of 16:37, 19 July 2018