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OD600 reference point | OD600 reference point | ||
− | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/thumb/f/fd/T--NU_Kazakhstan--CFUPlates.jpg/450px-T--NU_Kazakhstan--CFUPlates.jpg"> |
<h4>Conclusion</h4> | <h4>Conclusion</h4> | ||
Revision as of 13:35, 25 July 2018
InterLab
Every year, the Measurement Committee tries to analyze the causes of difference in values of fluorescence measurements obtained in laboratories around the year. Particularly in this year, the main goal of the Fifth International InterLaboratory Measurement Study is to investigate if the normalization of fluorescence measurements to the absolute cell count can contribute to the reduction of lab-to-lab variability in results. Our team, NU_Kazakhstan 2018, used the Varioskan LUX Multimode Microplate Reader to measure the fluorescence of DH 5 𝛼 cells transformed with the GFP inserted in pSB1C3 plasmid with different promoters (BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364007, BBa_J364008, BBa_J364009, plus negative BBa_R0040 and positive BBa_I20270 controls).
Protocol
We strictly followed the instructions from the protocol provided by iGEM https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf Plate reader configuration:Photometric
Wavelength: 600 nm Bandwidth: 5 nm Measurement time: 100 msFluorometric
Excitation Wavelength: 485 nm Emission Wavelength: 530 nm Excitation bandwidth: 12 nm Fluorescence reading: top optics Measurement time: 100 msResults
OD600 reference point![](https://static.igem.org/mediawiki/2018/thumb/f/fd/T--NU_Kazakhstan--CFUPlates.jpg/450px-T--NU_Kazakhstan--CFUPlates.jpg)