Difference between revisions of "Team:RHIT/InterLab"
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<h3>Bronze Medal Criterion #4</h3> | <h3>Bronze Medal Criterion #4</h3> | ||
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range. | <p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range. | ||
Revision as of 18:23, 26 July 2018
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Callibration
Before starting the experiments, our team calibrated the necessary equipment we would need for the InterLab study.
We calibrated the plate reader using serial dilutions of fluorescein. The table to the right represents the plate wells, and all values are measured in μM.
The table to the left shows our microsphere calibration measured at 630 nm.
The table to the right shows our absorbance calibration measured at 630nm. All values are measured in abs.
The following table represents our data from the CFU portion of InterLab. Samples of cells with an OD600 of 0.1 were diluted and grown on LB + chloramphenicol plates overnight. The next morning, the colonies were counted and used to predict the number of cells in 1 mL of media.
Bronze Medal Criterion #4
Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
For teams participating in the InterLab study, all work must be shown on this page.