Difference between revisions of "Team:DTU-Denmark/Notebook"

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<ol>
 
<ol>
 
   <li><p>To illustrate an examine the growthrate (for modelling)</p></li>
 
   <li><p>To illustrate an examine the growthrate (for modelling)</p></li>
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  <li>To multiply our pure species for use in liquid media.</li>
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  <li><p>To multiply our pure species for use in liquid media.</p></li>
 
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</ol>  
 
<p>
 
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Vogel's medium as well as a mineral salt medium as certain species had shown in previous study to grow well. However, we
 
Vogel's medium as well as a mineral salt medium as certain species had shown in previous study to grow well. However, we
 
did not have the proper materials to create those two and discarded the idea. We also came to this conclusion as we were
 
did not have the proper materials to create those two and discarded the idea. We also came to this conclusion as we were
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recommended not to spend time on specific media just yet.
+
recommended not to spend time on specific media just yet.<br>
  
 
All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
 
All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
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<div class="media-body">
 
<div class="media-body">
 
<h4>Wet Lab Overview</h4>
 
<h4>Wet Lab Overview</h4>
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<p>
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<p><b>20/6/2018</b><br>
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First day in lab was used for creating media of different types and concentrations for the species to be grown on for two
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Pleuratus ostreatus was re-inoculated on the solid agar plates by placement of around 8 mycelia "balls" under a fumehood and left at 30 degrees Celsius. As to save time, fewer plates were inoculated and a YBD plate was included.
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reasons:
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<br><br>Today, we had gotten another species, Schizophyllum commune, in two forms: a wildtype as well as a mutant (Δsc3) which were also inoculated on PDB, MEA and YBD plates.
 +
<br><br>
 +
<b>22/6/2018</b><br>
 +
First sign of growth in our species were showing and pictures were taken of every plate as to count pixels and indicate the growth rate. Pictures were taken every morning for 5 days.
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Revision as of 20:24, 29 July 2018

Notebook

Week 24 (June 10 - June 16)

  • Wet Lab Overview

    First day in lab was used for creating media of different types and concentrations for the species to be grown on for two reasons:

    1. To illustrate an examine the growthrate (for modelling)

    2. To multiply our pure species for use in liquid media.

    We used the media created in the "PDB (or PDA) and MEA media for species" protocol. It was also the plan to create Vogel's medium as well as a mineral salt medium as certain species had shown in previous study to grow well. However, we did not have the proper materials to create those two and discarded the idea. We also came to this conclusion as we were recommended not to spend time on specific media just yet.
    All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous. The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fumehood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.

Week 25 (June 17 - June 23)

  • Wet Lab Overview

    20/6/2018
    Pleuratus ostreatus was re-inoculated on the solid agar plates by placement of around 8 mycelia "balls" under a fumehood and left at 30 degrees Celsius. As to save time, fewer plates were inoculated and a YBD plate was included.

    Today, we had gotten another species, Schizophyllum commune, in two forms: a wildtype as well as a mutant (Δsc3) which were also inoculated on PDB, MEA and YBD plates.

    22/6/2018
    First sign of growth in our species were showing and pictures were taken of every plate as to count pixels and indicate the growth rate. Pictures were taken every morning for 5 days.

  • Dry Lab Overview

    All members are now full time on this iGEM project!
    This week we're working on completing the Poisson Hidden Markov model (HMM).
    The forms for the wet lab team's experiments is almost done and we hope to finish the welding so we can move on to test some of the physical properties.
    The last goal of the week is to find a fitting model for the mycelia growth and interactions and discuss the link to the wet lab experiments.

Week 26 (June 24 - July 1)

  • Wet Lab Overview

    First day in lab was used for creating media of different types and concentrations for the species to be grown on for two reasons:

  • Dry Lab Overview

    All members is now full time on this iGEM project!