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<p> The graph to the left plots the absorbance of each sample in the plate reader against its calculated CFU/mL value after plating the different dilutions. We noticed a general positive correlation between the variables, which makes sense considering a greater number of cells should have a higher absorbance value. We noticed more variability in the positive cultures than the negative cultures. The 3 positive cultures in the bottom left portion of the data are all samples from the first positive culture and the 3 in the top right portion of the data are the three samples from the second positive culture. </p> | <p> The graph to the left plots the absorbance of each sample in the plate reader against its calculated CFU/mL value after plating the different dilutions. We noticed a general positive correlation between the variables, which makes sense considering a greater number of cells should have a higher absorbance value. We noticed more variability in the positive cultures than the negative cultures. The 3 positive cultures in the bottom left portion of the data are all samples from the first positive culture and the 3 in the top right portion of the data are the three samples from the second positive culture. </p> | ||
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+ | <p> The box and whiskers plot to the left shows the variation in our calculated conversion factor to convert from an OD600 value to a CFU/mL value. It was determined by taking the calculated CFU values for each of the 12 samples and dividing by that sample's OD600 value as determined by the plate reader. The median values between both the positive and negative samples are close, suggesting little variability between a positive and negative sample when determining CFU calculations from an OD600 measurement. </p> | ||
Revision as of 17:02, 31 July 2018