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<p> Figure 1 and Figure 2 are calibration curves that show the means at each concentration of fluorescein. Figure 1 is shown with a standard scale, while Figure 2 is shown with a log scale. </p> | <p> Figure 1 and Figure 2 are calibration curves that show the means at each concentration of fluorescein. Figure 1 is shown with a standard scale, while Figure 2 is shown with a log scale. </p> | ||
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<p> Figure 3 and Figure 4 are calibration curves that show the arithmetic mean of the number of particles compared to the absorbance at OD600. Figure 3 is shown with a standard scale, while Figure 4 is shown with a log scale. </p> | <p> Figure 3 and Figure 4 are calibration curves that show the arithmetic mean of the number of particles compared to the absorbance at OD600. Figure 3 is shown with a standard scale, while Figure 4 is shown with a log scale. </p> | ||
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<h1> CFU Data </h1> | <h1> CFU Data </h1> | ||
<p> Table 8 represents our data from the CFU portion of InterLab. Samples of cells with an OD600 of ~0.1 were diluted and grown on LB + chloramphenicol plates overnight. The next morning, the colonies were counted and used to predict the number of colony forming units in 1 mL of media. </p> | <p> Table 8 represents our data from the CFU portion of InterLab. Samples of cells with an OD600 of ~0.1 were diluted and grown on LB + chloramphenicol plates overnight. The next morning, the colonies were counted and used to predict the number of colony forming units in 1 mL of media. </p> | ||
<img src = "https://static.igem.org/mediawiki/2018/0/0a/T--RHIT--CFUInterLab.jpg"> | <img src = "https://static.igem.org/mediawiki/2018/0/0a/T--RHIT--CFUInterLab.jpg"> | ||
− | <center> Table 8. Our counted colonies per plate as well as calculated CFUs/mL.</center | + | <center> Table 8. Our counted colonies per plate as well as calculated CFUs/mL. TNTC stands for Too Numerous to Count and indicates >300 colonies on the plate. </center> |
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<img src = "https://static.igem.org/mediawiki/2018/b/b1/T--RHIT--CFUgraph2.jpg"> | <img src = "https://static.igem.org/mediawiki/2018/b/b1/T--RHIT--CFUgraph2.jpg"> | ||
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<p> Figure 6 to the left plots the absorbance of each sample in the plate reader against its calculated CFU/mL value after plating the different dilutions. We noticed a general positive correlation between the variables, which makes sense considering a greater number of cells should have a higher absorbance value. We noticed more variability in the positive cultures than the negative cultures. The 3 positive cultures in the bottom left portion of the data are all samples from the first positive culture and the 3 in the top right portion of the data are the three samples from the second positive culture. </p> | <p> Figure 6 to the left plots the absorbance of each sample in the plate reader against its calculated CFU/mL value after plating the different dilutions. We noticed a general positive correlation between the variables, which makes sense considering a greater number of cells should have a higher absorbance value. We noticed more variability in the positive cultures than the negative cultures. The 3 positive cultures in the bottom left portion of the data are all samples from the first positive culture and the 3 in the top right portion of the data are the three samples from the second positive culture. </p> | ||
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<p> The box and whiskers plot to the left shows the variation in our calculated conversion factor to convert from an OD600 value to a CFU/mL value. It was determined by taking the calculated CFU values for each of the 12 samples and dividing by that sample's OD600 value as determined by the plate reader. The median values between both the positive control and negative control samples are close, suggesting little variability between a positive and negative sample when determining CFU calculations from an OD600 measurement. </p> | <p> The box and whiskers plot to the left shows the variation in our calculated conversion factor to convert from an OD600 value to a CFU/mL value. It was determined by taking the calculated CFU values for each of the 12 samples and dividing by that sample's OD600 value as determined by the plate reader. The median values between both the positive control and negative control samples are close, suggesting little variability between a positive and negative sample when determining CFU calculations from an OD600 measurement. </p> | ||
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Latest revision as of 14:12, 8 August 2018