Difference between revisions of "Team:SSHS-Shenzhen"

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<html lang="zh-CN">
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<html lang="en">
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<head>
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<head>
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    <meta charset="UTF-8">
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<meta http-equiv="Content-type" content="text/html;charset=UTF-8"/>
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    <title>Title</title>
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<meta name="generator" content="2018.0.0.379"/>
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</head>
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<meta name="viewport" content="width=device-width, initial-scale=1.0"/>
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<title>Home</title>
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<!-- CSS -->
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<style >
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<style>
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    /*解决上边和右边的东西*/
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html{min-height:100%;min-width:100%;-ms-text-size-adjust:none;}body,div,dl,dt,dd,ul,ol,li,nav,h1,h2,h3,h4,h5,h6,pre,code,form,fieldset,legend,input,button,textarea,p,blockquote,th,td,a{margin:0px;padding:0px;border-width:0px;border-style:solid;border-color:transparent;-webkit-transform-origin:left top;-ms-transform-origin:left top;-o-transform-origin:left top;transform-origin:left top;background-repeat:no-repeat;}button.submit-btn{-moz-box-sizing:content-box;-webkit-box-sizing:content-box;box-sizing:content-box;}.transition{-webkit-transition-property:background-image,background-position,background-color,border-color,border-radius,color,font-size,font-style,font-weight,letter-spacing,line-height,text-align,box-shadow,text-shadow,opacity;transition-property:background-image,background-position,background-color,border-color,border-radius,color,font-size,font-style,font-weight,letter-spacing,line-height,text-align,box-shadow,text-shadow,opacity;}.transition 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    #globalWrapper{
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        margin: 0;
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.version.index{color:#000009;background-color:#E68A6E;}#page{z-index:1;min-height:480px;background-image:none;border-width:0px;border-color:#000000;background-color:transparent;padding-bottom:20px;width:100%;max-width:1200px;margin-left:auto;margin-right:auto;}#ppu194{z-index:0;margin-right:-10000px;width:133.34%;}#pu194{z-index:2;width:0.01px;left:0px;margin-left:0px;}#u194_align_to_page{margin-left:auto;margin-right:auto;position:relative;}#u962{z-index:3;margin-bottom:-25px;position:relative;margin-right:-10000px;margin-top:-25px;width:11.84%;left:-1.42%;}.js body{visibility:hidden;}.js 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0px;}#u592{width:144px;min-height:38px;position:relative;margin-right:-10000px;left:879px;}#u593{width:144px;padding-bottom:16px;position:relative;}#u574.MuseMenuActive,#u593:hover{width:144px;min-height:0px;margin:0px;}#u593.MuseMenuActive{width:144px;min-height:0px;margin:0px;}#u594-4{width:144px;min-height:22px;position:relative;margin-right:-10000px;top:8px;}#u574.MuseMenuActive #u575-4,#u593:hover #u594-4{padding-top:0px;padding-bottom:0px;min-height:22px;width:144px;margin:0px -10000px 0px 0px;}#u593.MuseMenuActive #u594-4{padding-top:0px;padding-bottom:0px;min-height:22px;width:144px;margin:0px -10000px 0px 0px;}#u350{z-index:5;height:8px;}#u350-bw{z-index:5;height:8px;left:0px;margin-left:-200px;}#pu1092{z-index:53;width:100%;margin-left:-12.5%;}#u1092{z-index:53;width:1600px;background-color:transparent;position:relative;margin-right:-10000px;margin-left:50%;left:-800.0000000000002px;}#u1102-4{z-index:56;width:1200px;min-height:54px;background-color:transparent;line-height:60px;text-align:center;font-size:50px;font-weight:bold;position:relative;margin-right:-10000px;margin-top:120px;margin-left:50%;left:-600.0000000000002px;}#u981{z-index:55;height:80px;}#u981-bw{z-index:55;height:80px;left:0px;margin-left:-200px;}#u1103-4{z-index:60;min-height:162px;background-color:transparent;line-height:24px;font-size:20px;position:relative;width:52.38%;margin-left:11.32%;left:0px;}#u1111{z-index:64;height:80px;}.ie #u981,.ie #u1111{background-color:#FFFFFF;opacity:0.01;-ms-filter:"progid:DXImageTransform.Microsoft.Alpha(Opacity=1)";filter:alpha(opacity=1);}#u1111-bw{z-index:64;height:80px;left:0px;margin-left:-200px;}.css_verticalspacer .verticalspacer{height:calc(100vh - 836px);}#u984-4{z-index:49;min-height:13px;}#u984-4-bw{z-index:49;min-height:13px;margin-top:18px;}#muse_css_mq,.html{background-color:#FFFFFF;}body{position:relative;min-width:900px;}.verticalspacer{min-height:1px;}#u962_img{width:100%;}
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        padding: 0;
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    }
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    #content{
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        width: 100%;
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        padding: 0;
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        margin: -12px 0 0;
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    }
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    #top_title{
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        display: none;
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    }
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−
    #sideMenu{
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−
        display: none;
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    }
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−
    #content,
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    .vs-content,
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    .vs-wrapper section {
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−
        background-color: transparent !important;
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−
    }
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−
    .my-backTop:visited {
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−
        color: #F8A886 !important;
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−
    }
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−
    /*解决上边和右边的东西*/
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−
    body{background-color:#E8F1CB;
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−
    }
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−
    .header {
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        font-size: 30px;
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−
        padding:0px;
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−
        margin:0px;
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<p>Phyllotreta striolata killers</p>
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<p>Phyllotreta striolata is the most destructive insects worldwide. However, the present insect control strategies have certain limitations. We aim to develop a RNAi-based topical application approach. Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death. So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata. Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation. We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.</p>
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<p><span id="u984">2018 Shenzhen Senior High School iGEM</span></p>
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        <h2>Project Description</h2>
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        <p style="font-size:18px">This year, SMS_Shenzhen team will focus on using synthetic biologic method to prevent dental plaque. Dental plaque can be led by Streptococcus mutans, a bacteria lives in human's mouth. Clinging to the teeth in thin layers called biofilm, S. mutans digests sucrose and produces acids that can eat into enamel and cause cavities. Specifically, dextran is the main component of the biofilm. We find two enzymes, the first one is named "Dextranase", which can hydrolyze the dextran in the biofilm; and the second one is named "FruA", which can decompose the resource that S. mutans uses to produce bioflim. The gene of these two enzymes are cloned into E. coli and Lactobacillus. In our experiment, we would use E.coli to produce these two enzymes for relative measurement like enzyme activity. Then, for commercial design, we would produce leben with our Lactobacillus which can secrete these two enzymes.</p>
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Revision as of 07:26, 9 August 2018

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Phyllotreta striolata killers

Phyllotreta striolata is the most destructive insects worldwide. However, the present insect control strategies have certain limitations. We aim to develop a RNAi-based topical application approach. Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death. So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata. Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation. We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.

2018 Shenzhen Senior High School iGEM