Difference between revisions of "Team:DTU-Denmark/Experiments"

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<h1 style="position: absolute;
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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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  <p style="font-size: 70%;">All media was prepared before 11 am to be autoclaved. The actual weights of the different media was:<br><br>
  
 
<p>
 
<p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
 
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</p>
 
  
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<table class="tg">
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  <tr>
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    <th colspan="4"  style="background-color:#27518e;color:#ffffff;text-align:center;">MEA Media</th>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Materials</td>
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    <td class="tg-0lax">Weight (g)</td>
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    <td class="tg-0lax">Weight (g)</td>
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    <td class="tg-0lax">Weight (g)</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Malt extract</td>
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    <td class="tg-0lax">47.6</td>
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    <td class="tg-0lax">49.9</td>
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    <td class="tg-0lax">52.5</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">ZnSO4·7H2O</td>
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    <td class="tg-0lax">0.01</td>
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    <td class="tg-0lax">0.012</td>
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    <td class="tg-0lax">0.01</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">CuSO4·5H2O</td>
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    <td class="tg-0lax">0.05</td>
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    <td class="tg-0lax">0.049</td>
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    <td class="tg-0lax">0.051</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Agar</td>
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    <td class="tg-0lax">14.99</td>
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    <td class="tg-0lax">14.99</td>
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    <td class="tg-0lax">15.04</td>
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  </tr>
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  <tr>
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    <th style="background-color:#27518e;color:#ffffff;text-align:center;" colspan="4">PDB Media</th>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Potato dextrose extract</td>
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    <td class="tg-0lax">Weight (g)</td>
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    <td class="tg-0lax">Weight (g)</td>
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    <td class="tg-0lax">Weight (g)</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Malt extract </td>
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    <td class="tg-0lax">19.54</td>
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    <td class="tg-0lax">39.12</td>
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    <td class="tg-0lax">58.56</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">ZnSO4·7H2O</td>
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    <td class="tg-0lax">0.012</td>
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    <td class="tg-0lax">0.012</td>
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    <td class="tg-0lax">0.0107</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">CuSO4·5H2O </td>
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    <td class="tg-0lax">0.0056</td>
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    <td class="tg-0lax">0.0053</td>
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    <td class="tg-0lax">0.0049</td>
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  </tr>
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  <tr>
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    <td class="tg-mhe8">Agar</td>
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    <td class="tg-0lax">15.02</td>
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    <td class="tg-0lax">15.04</td>
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    <td class="tg-0lax">15.04</td>
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  </tr>
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</table>
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<p style="font-size: 70%;">
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All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.<br>
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The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick
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under a fume hood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia
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without any spores and the process was redone.
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</p>
 
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<h3>What should this page contain?</h3>
 
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<ul>
 
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<li> Protocols </li>
 
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<li> Experiments </li>
 
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<li> Documentation of the development of your project </li>
 
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</ul>
 
  
 
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<h3>Inspiration</h3>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
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</ul>
 
 
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Revision as of 08:30, 14 September 2018

Experiments

All media was prepared before 11 am to be autoclaved. The actual weights of the different media was:

MEA Media
Materials Weight (g) Weight (g) Weight (g)
Malt extract 47.6 49.9 52.5
ZnSO4·7H2O 0.01 0.012 0.01
CuSO4·5H2O 0.05 0.049 0.051
Agar 14.99 14.99 15.04
PDB Media
Potato dextrose extract Weight (g) Weight (g) Weight (g)
Malt extract 19.54 39.12 58.56
ZnSO4·7H2O 0.012 0.012 0.0107
CuSO4·5H2O 0.0056 0.0053 0.0049
Agar 15.02 15.04 15.04

All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fume hood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.