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Revision as of 10:36, 4 October 2018
A Synthetic Approach to Absorbing Copper Ions in Aquaponics
Heavy metal pollution has been a hot issue among the society, copper is one of the most universal type of pollutant. In aquaponics, accumulation of copper ions is toxic to organisms. In sight of this, we aimed to create a cost-effective device for the removal of metal ions in water. In this project, metallothionein, a type of protein capable of binding metal ions, was expressed in E. coli. The copper absorption capacity of the transformed bacteria is tested. From our results, E. coli can absorb copper ions at 10 mg/L and 2 mg/L. There is no significant difference between untransformed and transformed bacteria at 10 mg/L. At 2 mg/L, the transformed bacteria expressing Elsholtzia haichowensis Metallothionein 1 (EhMT1) slightly enhances the copper absorption ability. At last, we tried circulating E. coli inside a dialysis tubing, and received a positive result, it is confirmed that the idea of using bacteria to remove copper ions in water is feasible.
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Hong Kong JSS
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