Difference between revisions of "Team:Utrecht/Parts"
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<div class = "customelementM5A" id = "Introduction"> | <div class = "customelementM5A" id = "Introduction"> | ||
| − | <p>Several biobricks from the distribution kit were used during our project. These were retrieved from the kit using the protocols provided by iGEM<a class = "url_icon" href = "http://parts.igem.org/Help:2018_DNA_Distribution"></a>, transformed into <i>E. coli</i> and isolated in elution buffer NE. We also ordered several constructs from IDT (Table 1). These Biobricks were used to create several new biobricks that were relevant to our project. BBa_K2736000 | + | <p>Several biobricks from the distribution kit were used during our project. These were retrieved from the kit using the protocols provided by iGEM<a class = "url_icon" href = "http://parts.igem.org/Help:2018_DNA_Distribution"></a>, transformed into <i>E. coli</i> and isolated in elution buffer NE. We also ordered several constructs from IDT (Table 1). These Biobricks were used to create several new biobricks that were relevant to our project. BBa_K2736000<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736000"></a> and BBa_K2736001<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736001"></a>, were created to be used as BRET pair to measure the activity of our customized Tar receptors (BBa_K2736003<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736003"></a>-->, BBa_K2736004<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736004"></a>-->, and BBa_K2736005<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736005"></a>-->). To test whether it was possible to |
switch ligand binding domains of the Tar receptor and two component receptors we created | switch ligand binding domains of the Tar receptor and two component receptors we created | ||
BBa_K2736002<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736002"></a>-->, and BBa_K2736006<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736006"></a>-->.</p> | BBa_K2736002<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736002"></a>-->, and BBa_K2736006<!--<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736006"></a>-->.</p> | ||
Revision as of 10:33, 10 October 2018
Several biobricks from the distribution kit were used during our project. These were retrieved from the kit using the protocols provided by iGEM, transformed into E. coli and isolated in elution buffer NE. We also ordered several constructs from IDT (Table 1). These Biobricks were used to create several new biobricks that were relevant to our project. BBa_K2736000 and BBa_K2736001, were created to be used as BRET pair to measure the activity of our customized Tar receptors (BBa_K2736003, BBa_K2736004, and BBa_K2736005). To test whether it was possible to switch ligand binding domains of the Tar receptor and two component receptors we created BBa_K2736002, and BBa_K2736006.
| Name | Biobrick Number | Size | Size | Obtained |
|---|---|---|---|---|
| 1/5A::eYFP::CheY | BBa_K2736000 | 1865 | Chemotaxis responsive BRET pair (Part 1) | Created |
| 1/5A::CheZ::Rluc | BBa_K2736001 | 1655 | Chemotaxis responsive BRET pair (Part 2) | Created |
| Cu_prom::RFP | BBa_K2736002 | 930 | RFP behind a promoter that responds to CusR | Created |
| Tar(E491A) | BBa_K2736003 | 1703 | Tar receptor protein with a E->A substitution | Created |
| Tar(Q309A) | BBa_K2736004 | 1703 | Tar receptor protein with a Q->A substitution | Created |
| Tar(E491A, Q309A) | BBa_K2736005 | 1703 | Tar receptor with two amino acid mutations | Created |