Revision as of 18:54, 14 October 2018

Edinburgh iGEM 2018

General Protocols

Weigh 1 g of agarose
Add 100 mL of TAE 1X
Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down” and put it back on the heat until the agarose is completely dissolved
While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight
When homogeneous, add 10 µL of SYBR SAFE DNA Gel Stain to the solution and mix well
Pour the solution into the bed and clear all its bubbles with a pipette tip. Add in the comb and make sure it is secure
Mix the samples with loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well

Feel free to leave us a comment on social media!

@@ Line 119: / Line 119: @@
              <h1 class="brand-heading">General Protocols</h1>
               <h2 style="text-align:left">Agarose Gel</h2>
-                <ol><li> Weigh 1 g of agarose </li> <li>Add 100 mL of TAE 1X</li> <li>Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down”
+                <ol><li style="text-align:left"> Weigh 1 g of agarose </li> <li>Add 100 mL of TAE 1X</li> <li>Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down”
                   and put it back on the
                   heat until the agarose is completely dissolved</li> <li>While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight</li> <li>When