Difference between revisions of "Team:Oxford/protocols"
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Revision as of 18:52, 16 October 2018
Experiments
Protocols
We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.
Competent cells
Culturing cells
Colony PCR
Dilutions- gBlocks/ primers
Digests (DNA)
Gel preparation
Gel loading
Gel analysis and extraction
Ligation (DNA)
oePCR
PCR
Transformation