Difference between revisions of "Team:Uppsala/Phage Display"
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We used a Phage Display screening to create a biosensor that aggregated around the target molecule with the hypothesis that this would create a larger response. | We used a Phage Display screening to create a biosensor that aggregated around the target molecule with the hypothesis that this would create a larger response. | ||
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There are no readily available protocols for whole-organism exterior screenings using phage display, to read more closely what how we adapted this procedure to fit our needs read more here: | There are no readily available protocols for whole-organism exterior screenings using phage display, to read more closely what how we adapted this procedure to fit our needs read more here: | ||
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For this experiment we used a modified version of the protocol supplied with the kit from New England Biolabs. You can find the original protocol <a href="https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale8100.pdf">here</a>. | For this experiment we used a modified version of the protocol supplied with the kit from New England Biolabs. You can find the original protocol <a href="https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale8100.pdf">here</a>. | ||
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INTRO TEXT ABOUT OUR RESULTS - ots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... | INTRO TEXT ABOUT OUR RESULTS - ots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... | ||
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Here you can find our lab notes, the notes and scribbles we took during the course of our experiments: | Here you can find our lab notes, the notes and scribbles we took during the course of our experiments: | ||
Revision as of 22:57, 15 October 2018
Design
We used a Phage Display screening to create a biosensor that aggregated around the target molecule with the hypothesis that this would create a larger response.
Phage display is a method that is usually used to find a protein that binds to another protein. However using this technique on a whole eukaryotic organism is very uncommon, and therefore this project represents breaking new ground, albeit with the inevitable cost of hammering out the problems that come with such an undertaking.
In order to make our method work, we had to immobilize our worms and be able to extract the bound phages without also extracting the worms! Since our worms are far larger than a phage, we used an eppendorf filter insert that had pores too small for strongyles to pass through and allowed our phages to bind to these worms.
Using this process, we found several peptide sequences with potential high affinity to our worms. These sequences, after further validation, can in future be used to synthesize E. coli that will bind to our worms!
To read more about the general design of a Phage Display Screening and why we chose to apply this method, click here:
Experiment
There are no readily available protocols for whole-organism exterior screenings using phage display, to read more closely what how we adapted this procedure to fit our needs read more here:
Protocol
For this experiment we used a modified version of the protocol supplied with the kit from New England Biolabs. You can find the original protocol here.
Read more about our modified protocol here:
Results
INTRO TEXT ABOUT OUR RESULTS - ots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text... Lots and lots and lots and lots of example text...
Read more:
Notebook
Here you can find our lab notes, the notes and scribbles we took during the course of our experiments: