Stony Brook Team was one of the teams, who worked with the same strain of cyanobacteria Synechococcus elongatus PCC 7942. Collaboration with this team was very helpful for our team since the team shared their protocol of transforming cyanobacteria. At that moment, we troubleshooted the transformation protocol, media plate preparation protocols and antibiotic resistance for obtaining separate single colonies of transformed Synechococcus elongatus. The protocol of Stony Brook Team appeared to be successful and gave us first separate colonies which we could further use for the hydrogen sulfide assay. We, in turn, suggested some solutions for the adjustment of proper temperature for cyanobacteria in their CO2 incubator.