Difference between revisions of "Team:Munich/chiinhibition1.html"
ArianeKrus (Talk | contribs) |
ArianeKrus (Talk | contribs) |
||
| Line 19: | Line 19: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
<td>linear DNA was not degraded: we found out later that RecBCD was expired, but a positive control was missing.</td> | <td>linear DNA was not degraded: we found out later that RecBCD was expired, but a positive control was missing.</td> | ||
</tr> | </tr> | ||
| Line 45: | Line 45: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
<td>linear DNA was not degraded: We found out that RecBCD was expired and not functional.</td> | <td>linear DNA was not degraded: We found out that RecBCD was expired and not functional.</td> | ||
</tr> | </tr> | ||
| Line 70: | Line 70: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
<td>The agarose gel was prepared wrong. Therefore, samples were lost and could not be evaluated.</td> | <td>The agarose gel was prepared wrong. Therefore, samples were lost and could not be evaluated.</td> | ||
</tr> | </tr> | ||
| Line 117: | Line 117: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
<td>Chi6 oligomers inhibit RecBCD but are degraded within 30min. We went on to show these results in cell extract.</td> | <td>Chi6 oligomers inhibit RecBCD but are degraded within 30min. We went on to show these results in cell extract.</td> | ||
</tr> | </tr> | ||
Revision as of 13:08, 16 October 2018
In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/15| Participants: | Dominic Schwarz |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Results: | linear DNA was not degraded: we found out later that RecBCD was expired, but a positive control was missing. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/06/19| Participants: | Enikö Baligács |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | The experiment was redone by another student and a positive control was added. |
| Results: | linear DNA was not degraded: We found out that RecBCD was expired and not functional. |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/07/02| Participants: | Julia Mayer |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | |
| Results: | The agarose gel was prepared wrong. Therefore, samples were lost and could not be evaluated. |
linearizing pSB1C3_RFP
2018/08/01| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification |
| Notes: | EcoRI-HF |
Redo: In Vitro inhibition of RecBCD with Chi6 Oligomers
2018/08/02| Participants: | Enikö Baligács, Julia Mayer |
| Protocol: | Agarose gel, RecBCD Inhibition in vitro |
| Notes: | The experiment was repeated with newly ordered recBCD |
| Results: | Chi6 oligomers inhibit RecBCD but are degraded within 30min. We went on to show these results in cell extract. |
