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− | <td> | + | <td>Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.</td> |
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Revision as of 19:20, 16 October 2018
Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering
2018/08/28Participants: | Dominic Schwarz |
Protocol: | Electrocompetent transformation |
Notes: | Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid. |
Results: | ~200 colonies |