Difference between revisions of "Team:Munich/deletioncasette.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electro-transformation</a> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>No notes.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
<td>No colonies, no growth; <br> | <td>No colonies, no growth; <br> | ||
| − | We decided to use a resistance cassette from pSB1C3 without FRT sites | + | We decided to use a resistance cassette from pSB1C3 without FRT sites. |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 11:09, 17 October 2018
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
2018/05/28| Participants: | Dominic Schwarz |
| Protocol: | Electro-transformation |
| Notes: | No notes. |
| Results: | No colonies, no growth; We decided to use a resistance cassette from pSB1C3 without FRT sites. |
Amplifying a selection cassette from pSB1C3
2018/05/29| Participants: | Enikö Baligács |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Primer with 50bp homology regions ????, TA: 67°C, Elongation: 45s |
| Results: | PIC. We thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |