Difference between revisions of "Team:Aix-Marseille/Basic Part"
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| − | Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide) | + | Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). [http://parts.igem.org/Part:BBa_K2718022 BBa_K2718005] is in RFC 10, and part comes from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300].To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [https://www.dovepress.com/activities-of-methionine-gamma-lyase-in-the-acidophilic-archaeon-ldquo-peer-reviewed-article-RRB]. |
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| + | https://static.igem.org/mediawiki/parts/1/16/--Aix-Marseille--Shcema_MGL_parts.jpg | ||
=BBa_K2718020= | =BBa_K2718020= | ||
=BBa_K2718021= | =BBa_K2718021= | ||
Revision as of 13:27, 17 October 2018
Basic Parts
Basic Parts
A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.
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BBa_K2718005
Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). BBa_K2718005 is in RFC 10, and part comes from BBa_K1493300.To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [1].
