Difference between revisions of "Team:Aix-Marseille/Composite Part"
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| − | + | We constructed 4 composite parts during this years iGEM competition. Three of them were made by attaching a promoter (with or without a ribosome binding site) in front of another part. The last was an RBS coding sequence fusion that we had synthesised by IDT. | |
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Revision as of 15:34, 17 October 2018
Composite Parts
We constructed 4 composite parts during this years iGEM competition. Three of them were made by attaching a promoter (with or without a ribosome binding site) in front of another part. The last was an RBS coding sequence fusion that we had synthesised by IDT.
BBa_K2718006
Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). BBa_K2718005 is in RFC 10, and part comes from BBa_K1493300. Promotor is BBa_R0011, it's IPTG inducible promotor. To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [1].
