Difference between revisions of "Team:Aix-Marseille/Basic Part"

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<h1>Basic Parts</h1>
 
 
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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We made three basic parts during this years iGEM competition coding for 2 different enzymes.
 
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=BBa_K2718005=
 
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Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). [http://parts.igem.org/Part:BBa_K2718022 BBa_K2718005] is in RFC 10, and part comes from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300].To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [https://www.dovepress.com/activities-of-methionine-gamma-lyase-in-the-acidophilic-archaeon-ldquo-peer-reviewed-article-RRB].
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Methionine gamma-lyase (MGL) catalyzes the conversion of l-methionine into methanthiol, then the oxidation of methanethiol produces DMDS (Dimethyl disulfide) and DMTS ( Dimethyl trisulfide). [http://parts.igem.org/Part:BBa_K2718005 BBa_K2718005] is an RFC 10 compatible part that we derived from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300] using PCR to add a his tag. To test activity we used the compositive part [http://parts.igem.org/Part:BBa_K2718006 BBa_K2718006]
  
 
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https://static.igem.org/mediawiki/parts/1/16/--Aix-Marseille--Shcema_MGL_parts.jpg

Revision as of 16:09, 17 October 2018

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Basic Parts

We made three basic parts during this years iGEM competition coding for 2 different enzymes.

.

BBa_K2718005

Methionine gamma-lyase (MGL) catalyzes the conversion of l-methionine into methanthiol, then the oxidation of methanethiol produces DMDS (Dimethyl disulfide) and DMTS ( Dimethyl trisulfide). BBa_K2718005 is an RFC 10 compatible part that we derived from BBa_K1493300 using PCR to add a his tag. To test activity we used the compositive part BBa_K2718006

--Aix-Marseille--Shcema_MGL_parts.jpg

BBa_K2718020

Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere to . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli. Part is RFC 25 standards. We test activiy with Schales' procedure [1].

BBa_K2718021

Chitinase BBa_K2718020is a fungus (Beauveria bassiana) enzyme that degrads chitin. It's endochitinase wich hydrolyses 1-4 beta link between N-acetyl-D-glucosamine monomere . Sequence comes from uniprot, we used peptidic sequence between 41 to 330 AA, that's correspond to catalitic domain. We improve nucleotidic sequence with IDT tool for synthesis by E.coli, and there is his tag in C-ter. Part is RFC 25 standards. We test activiy with Schales' procedure[2].