Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"
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Protocols for PCR | Protocols for PCR | ||
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Protocols for purification | Protocols for purification | ||
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Protocols for digestion and assembly | Protocols for digestion and assembly | ||
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Protocols for <i>Saccharomyces cerevisiae</i> | Protocols for <i>Saccharomyces cerevisiae</i> | ||
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Protocols for <i>Escherichia coli</i> | Protocols for <i>Escherichia coli</i> | ||
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Protocols for Biobricks | Protocols for Biobricks | ||
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Protocols for Interlab study | Protocols for Interlab study | ||
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Revision as of 18:18, 17 October 2018
Protocols
Workflow Overview
In the figure below an overview of the cloning process, shared by all project parts, is presented.
Protocols for PCR
Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR
Protocols for purification
Thermo Scientific GeneJET PCR Purification Kit
Thermo Scientific GeneJET Gel Extraction Kit
Protocols for digestion and assembly
FastDigest Restriction Enzymes
Gibson Assembly Master Mix
DNA Insert Ligation into Vector DNA
Protocols for Saccharomyces cerevisiae
Transformation (Chemical)
Transformation (Quick version)
Isolation of genomic DNA
Protocols for Escherichia coli
Transformation (Heat shock)
Thermo Scientific GeneJET Plasmid Miniprep Kit