Difference between revisions of "Team:Uppsala/Reporter System/Feces Analysis"

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                 <li class="toclevel tocsection"><a href="#Project_Description" class="scroll"> <span id="whereYouAre"> Project Description  </span> </a>
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                 <li class="toclevel tocsection"><a href="#Project_Description" class="scroll"> <span id="whereYouAre"> Reporter System</span> </a>
 
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                             <li class="toclevel nav-item active"><a href="#top" class="nav-link scroll"> Overview </a></li>
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                             <li class="toclevel nav-item active"><a href="#Fec" class="nav-link scroll"> Design of Feces Analysis</a></li>
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                             <li class="toclevel nav-item"><a href="#Resu" class="nav-link scroll">  Results</a></li>
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                             <li class="toclevel nav-item"><a href="#Conc" class="nav-link scroll">  Conclusion</a></li>
 
                             <li class="toclevel nav-item"><a href="#References" class="nav-link scroll"> References </a></li>
 
                             <li class="toclevel nav-item"><a href="#References" class="nav-link scroll"> References </a></li>
 
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                 <h1>Design of Feces Analysis</h1>
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<p>Regarding the second experiment aimed to establish what concentrations would be required in order for fluorescence to be measured with a plate reader. The results can be seen in figure 3 and figure 4 below. Figure 3 shows the fluorescence of samples where feces were mixed with amilGFP and figure 4 shows the fluorescence of the filtrate. The results where feces had been mixed with liquid culture of amilGFP show inconclusive results, with no relationship between the concentration of amilGFP and the fluorescence intensity. However, the results from the samples where liquid cultures of amilGFP, LB-media and feces were mixed and filtered through a coffee filter and a Munktell filter shows some linearity in the fluorescence intensity.
 
<p>Regarding the second experiment aimed to establish what concentrations would be required in order for fluorescence to be measured with a plate reader. The results can be seen in figure 3 and figure 4 below. Figure 3 shows the fluorescence of samples where feces were mixed with amilGFP and figure 4 shows the fluorescence of the filtrate. The results where feces had been mixed with liquid culture of amilGFP show inconclusive results, with no relationship between the concentration of amilGFP and the fluorescence intensity. However, the results from the samples where liquid cultures of amilGFP, LB-media and feces were mixed and filtered through a coffee filter and a Munktell filter shows some linearity in the fluorescence intensity.
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<p>It would be interesting to redo the experiment and to see if there would be any change over time.  Other settings for the plate reader also might yield different results.  Furthermore it would be interesting to redo the experiment with lower concentrations and a smaller interval to come to a conclusion on what the lowest amount bacteria that is required for detection of fluorescent bacteria in feces is. There was also some inconsistency in the fluorescent intensity measured between triplicate samples, and more duplicates would likely make the results more accurate. </p>
 
<p>It would be interesting to redo the experiment and to see if there would be any change over time.  Other settings for the plate reader also might yield different results.  Furthermore it would be interesting to redo the experiment with lower concentrations and a smaller interval to come to a conclusion on what the lowest amount bacteria that is required for detection of fluorescent bacteria in feces is. There was also some inconsistency in the fluorescent intensity measured between triplicate samples, and more duplicates would likely make the results more accurate. </p>
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<h2>Reference</h2>
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<p><b>[1]</b> Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynnå A, Ivermark K, Lövgren J, Törnblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. 2018. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering</p>
 
<p><b>[1]</b> Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynnå A, Ivermark K, Lövgren J, Törnblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. 2018. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering</p>
  

Revision as of 23:16, 17 October 2018