Difference between revisions of "Team:Uppsala/Worm Culturing"

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                     <p>To investigate the possibility of adapting the workflow of the development of the diagnosis tool for small strongyles to large strongyles, samples containing uniquely large strongyles are needed. </p>
 
                     <p>To investigate the possibility of adapting the workflow of the development of the diagnosis tool for small strongyles to large strongyles, samples containing uniquely large strongyles are needed. </p>
                     <p>Since small strongyles can be present in horses without large strongyles, but the opposite doesn’t occur, a system to separate the two types of strongyles was needed. The only pre-existing system employed by researchers that intend to collect nematodes of a certain class is the use of a pick under a microscope. This however is very time consuming and doesn’t allow obtaining a high concentration of nematodes and therefore a different procedure was needed. With this goal a microfluidic chip has been created. </p>
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                     <p>Since small strongyles can be present in horses without large strongyles, but the opposite doesn’t occur, a system to separate the two types of strongyles was needed. The only pre-existing system employed by researchers that intend to collect nematodes of a certain class is the use of a pick under a microscope. This however is very time consuming and doesn’t allow obtaining a high concentration of nematodes and therefore a different procedure was needed. With this goal a microfluidic chip has been created by 3D-printing a mold to cast the chip in. </p>
 
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<p align="center"><b>Figure 6.</b> Microfluidics chip.<br></p>
 
<p align="center"><b>Figure 6.</b> Microfluidics chip.<br></p>
 
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<p>The chip presented a single channel that splits in 2. The solution containing both types of nematodes, and any kind of contaminant, can be pumped inside the single channel. 2 valves, connected to the 2 split channels, can be used to redirect the flow from the main channel to one of the 2. Positioning this chip under a microscope, and analysing a strongyle when reaching the bifurcation allows the determination of the nature of the nematode. The flow is then directed towards the collection tubes. </p>  
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<p>The chip presented a single channel that splits in two. The solution containing both types of nematodes, and any kind of contaminant, can be pumped inside the single channel. Two valves, connected to the two split channels, can be used to redirect the flow from the main channel to one of the two. Positioning this chip under a microscope, and analysing a strongyle when reaching the bifurcation allows the determination of the nature of the nematode. The flow is then directed towards the collection tubes. </p>  
 
<p>This system, even though it doesn’t allow an immediate separation of all the large strongyles from the sample, leads to a quicker separation compared to hand picking. A low flow pump, such as a pressure or a syringe pump, is required. These details are described in the protocol we developed.</p>
 
<p>This system, even though it doesn’t allow an immediate separation of all the large strongyles from the sample, leads to a quicker separation compared to hand picking. A low flow pump, such as a pressure or a syringe pump, is required. These details are described in the protocol we developed.</p>
 
                  
 
                  

Revision as of 01:05, 18 October 2018