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| − | <section>
| + | <embed width="1000px" height="1200px" src="https://static.igem.org/mediawiki/2018/a/a6/T--SIAT-SCIE--SIAT_Protocals.pdf" alt="Protocals" /> |
| − | <article>
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| − | <details style="margin-top: 40px;">
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| − | <summary>PCR</summary>
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| − | <h> PCR condition</h>
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| − | <p>
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| − | <ul>
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| − | <li>Before cycles: 98°C 10
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| − | </li>
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| − | <li>After cycles: 72°C 7min; 16°C ∞ (4°C is more suitable for storing PCR product, but 4°C overnight may damage some PCR machine)
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| − | (A) For reactions in which the quantity of template is 200 ng / 50 μl or less:*
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| − | </li>
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| − | <li>-98°C 10 sec.
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| − | </li>
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| − | <li>-55°C 5 sec. or 15 sec
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| − | </li>
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| − | <li>-72°C 5 sec./kb
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| − | </li>
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| − | <li>-30-35 cycles
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| − | </li>
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| − | <br/>
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| − | <br/>
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| − | <li>(B) For reactions in which the quantity of template exceeds 200 ng / 50 μl:*
-98°C 10 sec.
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| − | </li>
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| − | <li>-55°C 5 sec. or 15 sec.
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| − | </li>
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| − | <li>-72°C 30 - 60 sec./kb
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| − | </li>
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| − | <li>-30-35 cycles
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| − | </li>
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| − | </p>
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| − | </details>
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| − | <details>
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| − | <summary>Agarose Gel Electrophoresis </summary>
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| − | <p>After setup the program doesn't start</p>
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| − | </details>
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| − | </article>
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| − | </section>
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