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Interlab Study

OVERVIEW

A challenge of synthetic biology is repeating measurements in different laboratories. For example, fluorescence data is difficult to compare either because it is reported in different units, or because different groups handle raw data differently. iGEM’s Measurement Committee thus aims to use the InterLab Study to eventually develop absolute units for measurements of green fluorescent protein (GFP) in a plate reader. This will improve the measurement tools of synthetic biologists.

This year, the Committee aims to discover if it is possible to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of optical density (OD). For this, we were required to measure the cell density of Escherichia coli ( E.Coli ) DH5⍺ cells using the methods below.

Method 1: Converting between absorbance of cells to absorbance of a known concentration of beads

In the first method, silica beads are used to estimate the actual amount of cells during fluorescence measurement. These beads are modeled after a typical E. coli cell and are thus expected to scatter light in a similar way to E. Coli cells. As a sample of these silica beads gives a consistent and known absorbance measurement at 600 nm, absorbance measurements from a sample’s cell density can be converted into an “equivalent concentration of beads” measurement that should be more universal and comparable between different labs.

Method 2: Counting colony-forming units (CFUs) from the sample

In the second method, cell concentration is approximated is by plating a known volume of the sample and letting bacterial colonies grow. As each bacterial colony is assumed to represent a single cell (for cells that do not stick together), the cell concentration in the sample is then directly proportional to the number of CFUs. Using a scaling factor computed from negative and positive control CFUs, a conversion factor from absorbance to CFU can be computed.

PARTS RECEIVED

Device	Part Number	Usage
Negative control	BBa_R0040	TetR repressible promoter, medium strength promoter
Positive Control	BBa_I20270	Promoter MeasKit (J23151)
Test Device 1	BBa_J364000	GFP expressing constitutive device
Test Device 2	BBa_J364001	GFP expressing constitutive device
Test Device 3	BBa_J364002	GFP expressing constitutive device
Test Device 4	BBa_J364007	Expresses GFP under the control of a constitutive promoter
Test Device 5	BBa_J364008	Expresses GFP under the control of a constitutive promoter
Test Device 6	BBa_J364009	Expresses GFP under the control of a constitutive promoter

MATERIALS & METHODS

Abs₆₀₀ --

Wavelength: 600nm
Read Speed: Normal
Delay: 100 msec

Fluorescence --

Excitation: 485
Emission: 525
Optics: Top
Gain: 50
Light Source: Xenon Flash
Lamp Energy: High
Read Speed: Normal
Delay: 100 msec
Read Height: 7 mm

Materials

1 ml LUDOX CL-X
ddH₂O
96-well plate (black)

Method

100 µl of LUDOX was added into wells A1, B1, C1 and D1.
100 µl of ddH20 was added into wells A2, B2, C2 and D2.
Abs_600nm was measured for all samples.

Results	LUDOX CL-X	H₂O
Replicate 1	0.056	0.037
Replicate 2	0.056	0.038
Replicate 3	0.056	0.038
Replicate 4	0.055	0.038
Arth. Mean	0.056	0.038
Corrected Abs₆₀₀	0.018
Reference OD₆₀₀	0.063
OD₆₀₀/Abs₆₀₀	0.063

Materials

300 µl Silica beads (4.7 x 108 microspheres)
ddH₂O
96-well plate (black)

Methods (A) To prepare the Microsphere Stock Solution

Tube labelled “Silica Beads” was vortexed vigorously for 30 s.
96 µl of microspheres was immediately pipetted into a 1.5 ml eppendorf tube.
904 µl of ddH₂0 was added to the microspheres. The eppendorf was vortexed well.

(B) To prepare the serial dilution of microsphere

100 µl of ddH20 was added into wells A2, B2, C2, D2...A12, B12, C12, D12.
The microsphere stock solution was vortexed vigorously for 10 s before immediately adding 200 µl of microspheres into A1.
100 µl of microsphere stock solution was transferred from A1 to A2.
Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3.
The subsequent dilutions were prepared as illustrated on Image A (below).
Samples were re-mixed immediately before putting it in the plate reader. Fluorescence_(Abs600) all samples were measured.
Fluorescence (Abs₆₀₀ ) of all samples were measured. Our results are reflected by Fig. 2 and Fig. 3.

Materials

Fluorescein
10 ml 1X PBS pH 7.4 - 7.6
96-well plate (black)

Methods
(A) To prepare the fluorescein stock solution

The fluorescein kit tube was spun down to make sure that the pellet was collected at the bottom of the tube.
10X fluorescein stock solution (100 µM) was prepared by resuspending fluorescein in 1 ml of 1X PBS. Fluorescein was checked to be properly dissolved in PBS by checking for no more visible particulates in the pipette tip when resuspending.
10X fluorescein stock solution was diluted with 1X PBS to make a 1X fluorescein solution (10 µM): 100 µl of 10X fluorescein stock solution was mixed with 900 µl of 1X PBS.

// add picture

(B) To prepare the serial dilution of fluorescein

100 µl of PBS was added into wells A2, B2, C2, D2...A12, B12, C12, D12.
200 µl of 1X fluorescein stock solution was added into A1, B1, C1 and D1.
100 µl of 1X fluorescein stock solution was transferred from A1 to A2.
Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3.
The subsequent dilutions were prepared as illustrated by Fig. 4.
Fluorescence of all samples were measured, and our results are reflected by Fig. 5.

// add picture

Materials

Competent cells (Escherichia coli strain DH5α)
Luria Bertani (LB) media
Chloramphenicol (stock concentration 25 mg/ml dissolved in ethanol)
50 ml Falcon tube (wrapped with a thick layer of paper towel to block light)
1.5 ml eppendorf tubes
Incubator at 37 °C
Ice bucket with ice
96-well plate (black)

Methods Day 1: Transforming Escherichia coli strain DH5α with devices provided in the Distribution Kit

Each device (powder form) was resuspended in 10 µl of ddH₂O.
1 µl of each respective plasmid was transformed into 50 µl E. coli DH5α via electroporation.
500 µl of LB was immediately added into each tube for recovery of the transformed cells.
Each sample tube was incubated with shaking at 37 °C for 30 mins before 100 µl of each sample was plated onto LB + Chlor agar plates and grown overnight at 37 °C.

Day 2: Selecting Colonies and Growing Cells Overnight

2 colonies were selected for being both relatively bigger and more separated from the other colonies.
The selected colonies were then inoculated into 5 ml of LB + Chlor and grown overnight at 37 °C at 220 rpm.

Day 3: Cell Growth, Sampling and Assay

Part 1: Abs₆₀₀nm and Fluorescence Measurement

A cell stock of each overnight culture was made in glycerol for storage, in case there is a need to use them again. To make this stock, 850 µl of culture was added to 350 µl of glycerol.
A 1:10 dilution of each overnight culture was made in LB + Chlor (0.5 mL culture + 4.5 mL media).
Abs₆₀₀nm of the 1:10 diluted cultures were measured.
Cultures were diluted further to a target Abs600nm of 0.02 in a final volume of 12 ml LB + Chlor in a 50 ml falcon tube that was covered with tissue paper.
500 µl of samples of the diluted cultures at 0 h were transferred into 1.5 ml eppendorf tubes labelled A and B. The tubes were placed on ice until they were ready to be laid out according to the plate diagram to measure fluorescence and Abs600. Fluorescence readings at T = 0 h are shown in Fig. 6.
The rest of the cultures were incubated at 37 °C and 220 rpm for 6 hours.
After the 6-hour-incubation, 500 µl of these cultures were transferred into 1.5 ml eppendorf tubes before being laid out according to the plate diagram below. The samples’ fluorescence and Abs600nm were measured again. Fluorescence readings at T = 6 h are shown in Fig. 7.

Part 2: Colony Forming Units per 0.1 OD600 E. coli Cultures

Only Positive Control (BBa_I20270) cultures and Negative Control (BBa_R0040) were involved in this part.

Overnight cultures were diluted 10-fold in LB + Chlor media to ensure they lay in the linear detection range of our plate reader.
The OD₆₀₀nm of cell cultures were measured. Our results are reflected by Fig. 8.
Overnight cultures were diluted to OD₆₀₀ = 0.1 in 1 ml of LB + Chlor media. Each culture was done in triplicate.
Diluted overnight cultures were checked to ensure that OD600 = 0.1, excluding the blank measurement
For each starting sample, serial dilutions were prepared as shown in Fig. 9.
100 µl of Dilutions 3, 4 and 5 were aseptically spread on LB + Chlor agar plates.
The plates were incubated overnight at 37 °C.
Colonies on each plate were counted. Our results are reflected by Fig. 10.

Fluorescence Raw Readings

Hour 0 Nge. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)

Colony 1, Replicate 1 34 76 361 122 53 318 289 88 47

Colony 1, Replicate 2 71 67 418 131 42 338 283 57 34

Colony 1, Replicate 3 52 66 386 127 44 309 304 90 27

Colony 1, Replicate 4 53 101 391 112 45 367 314 94 33

Colony 2, Repplicate 1 65 61 357 100 8 329 277 76 35

Colony 2, Replicate 2 47 83 389 144 24 339 2278 91 38

Colony 2, Replicate 3 79 74 377 138 33 335 280 85 45

Colony 2, Replicate 4 61 65 350 142 45 327 282 72 31

Hour 6 Nge. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)

Colony 1, Replicate 1 36 461 1252 920 58 2389 338 436 35

Colony 1, Replicate 2 45 500 1351 988 55 2621 354 433 54

Colony 1, Replicate 3 53 501 1390 1014 60 2757 393 446 24

Colony 1, Replicate 4 61 488 1332 1039 36 2793 351 448 31

Colony 2, Repplicate 1 30 429 971 821 44 2142 371 437 8

Colony 2, Replicate 2 55 516 1199 832 96 2390 378 401 24

Colony 2, Replicate 3 38 483 1239 838 74 2401 425 423 40

Colony 2, Replicate 4 49 460 1129 896 57 2528 384 423 51

OD₆₀₀ Raw Readings

Hour 0: Nge. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)

Colony 1, Replicate 1 0.061 0.06 0.061 0.063 0.059 0.059 0.063 0.061 0.043

Colony 1, Replicate 2 0.062 0.062 0.063 0.063 0.063 0.062 0.065 0.063 0.043

Colony 1, Replicate 3 0.063 0.062 0.064 0.065 0.064 0.061 0.063 0.061 0.043

Colony 1, Replicate 4 0.062 0.062 0.065 0.063 0.063 0.062 0.063 0.062 0.043

Colony 2, Repplicate 1 0.06 0.063 0.064 0.063 0.062 0.062 0.062 0.064 0.045

Colony 2, Replicate 2 47 83 389 144 24 339 2278 91 38

Colony 2, Replicate 3 0.061 0.063 0.061 0.065 0.061 0.065 0.06 0.062 0.046

Colony 2, Replicate 4 0.061 0.063 0.061 0.065 0.061 0.065 0.06 0.062 0.046

Hour 6 Nge. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)

Colony 1, Replicate 1 0.428 0.415 0.174 0.445 0.455 0.418 0.074 0.432 0.042

Colony 1, Replicate 2 0.452 0.435 0.19 0.481 0.481 0.46 0.08 0.469 0.042

Colony 1, Replicate 3 0.459 0.434 0.198 0.495 0.487 0.462 0.081 0.46 0.042

Colony 1, Replicate 4 0.461 0.439 0.185 0.495 0.479 0.477 0.076 0.467 0.043

Colony 2, Repplicate 1 0.446 0.414 0.138 0.434 0.443 0.406 0.079 0.419 0.043

Colony 2, Replicate 2 0.467 0.447 0.149 0.452 0.484 0.437 0.076 0.435 0.043

Colony 2, Replicate 3 0.496 0.448 0.153 0.449 0.485 0.455 0.082 0.435 0.043

Colony 2, Replicate 4 0.483 0.444 0.148 0.475 0.467 0.455 0.079 0.409 0.04

Counts for Colony-Forming Units

D4 8 x 10⁵ NCAI NCA2 NCA3 NCB1 NCB2 NCB3 Avg NCA Avg NCB

445 286 649 173 608 945 4.60E + 02 5.75E + 02

PCAI PCA2 PCA3 PCB1 PCB2 PCB3 Avg PCA Avg PCB

195 219 245 232 250 253 2.20E + 02 2.45E + 02

D5 8 x 10⁵ NCAI NCA2 NCA3 NCB1 NCB2 NCB3 Avg NCA Avg NCB

49 32 89 32 153 418 5.67E + 01 2.01E + 02

PCAI PCA2 PCA3 PCB1 PCB2 PCB3 Avg PCA Avg PCB

36 54 35 29 4 27 4.17E + 02 2.00E + 02

Cell Concentration

D4 8 x 10⁵ NCAI NCA2 NCA3 NCB1 NCB2 NCB3 Avg NCA Avg NCB

3.52E + 09 2.24E + 09 5.14E + 09 1.41E + 09 4.72E + 09 7.49E + 09 3.64E + 09 4.54E + 09

PCAI PCA2 PCA3 PCB1 PCB2 PCB3 Avg PCA Avg PCB

1.61E + 09 1.95E + 09 2.11E + 09 1.70E + 09 1.85E + 09 2.25E + 09 1.89E + 09 1.93E + 09

D5 8 x 10⁵ NCAI NCA2 NCA3 NCB1 NCB2 NCB3 Avg NCA Avg NCB

3.88E + 09 2.51E + 09 7.05E + 09 2.61E + 09 1.19E + 10 3.31E + 10 4.48E + 09 1.59E + 10

PCAI PCA2 PCA3 PCB1 PCB2 PCB3 Avg PCA Avg PCB

2.97E + 09 4.80E + 09 3.01E + 09 2.13E + 09 2.96E + 08 2.40E + 09 3.59E + 09 1.61E + 09

CFU/mL/OD

Avg of D4 and D5

NCA 4.06E + 09

NCB 1.02E + 10

PCA 2.74E + 09

PCB 1.77E + 09

Materials

SpheroTech Rainbow calibration beads, type RCP-30-5A (Lot Number: AAF02)

Flow cytometer set to collect 10,000 events per well or a read time of 1 min per well

Methods

A sample of SpheroTech beads was prepared according to the manufacturer instructions

Plate setup was as shown below.

DISCUSSION
Abs₆₀₀ nm

Rate of growth is inferred from Net Abs 600 nm values; Net Abs 600 nm is a common method of measuring cell concentration. All cells except for cells transformed with Devices 1 and 5 had comparable rates of growth. In cells transformed with Devices 1 and 5, the increase in Net Abs 600 nm was significantly slower than cells transformed with the rest of the devices (see Figure Z). //add pictures

µM Fluorescein per OD

Cells transformed with Devices 1, 4 and 5 had the highest fluorescein readings per OD.

µM fluorescein per OD of cells transformed with Device 3 were very low and at levels comparable to cells transformed with the Negative Control.

Cells transformed with Devices 2 and 6 had similar µM fluorescein per OD to that of cells transformed with the Positive Control.

Similar trends were observed for MEFL/particle.

Derivations/Inferences made about devices

Despite growing at a considerably slower rate, fluorescein readings per OD of cells transformed with Devices 1 and 5 were among the highest. The high GFP production in cells transformed with Devices 1 and 5 might have caused a unhealthy metabolic stress on the cells, which, in turn, could have impeded the growth of the cells.

Cells transformed with Device 4 had a slightly lower fluorescein reading per OD as compared to cells transformed with Devices 1 and 5. Since there was no compromise on the cell growth rate despite the high GFP production, the slightly lower GFP production (as compared to cells transformed with Devices 1 and 5) was inferred to have permitted the cells to grow at normal rates, leading to an overall higher net fluorescein reading.

Cells transformed with Device 3 was observed to produce very little to no GFP. Promoter within Device 3 might have been repressed by a molecule present in the cells, or not activated, to result in minimal GFP expression.

Conclusion about devices
Devices 1 and 5 were inferred to have highest promoter strength upstream of the GFP gene. While they produced the highest fluorescein readings per OD, overexpression of GFP as a result of Devices 1 and 5 do not seem to be healthy for cells: cells were not observed to be able to cope with the overly high expression of GFP. In the same argument, Device 4 seemed to be the most advantageous for the experimenter: there was a high GFP production without a compromise of growth rate. Cells transformed with Device 4 seemed to be able to manage the level of metabolic stress and grow normally.

CFU/mL/OD
Based on the data, NCA, NCB, PCA, PCB averages were computed based on counted CFU units. The percentage errors computed based on the known concentrations of beads against their respective averages showed high error levels.
// Confirm with wiki team

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InterLab

Bronze Medal Criterion #4

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-  <h1>Interlab Study</h1>
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-  <h2 style="padding-left: 0;">OVERVIEW</h2>
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-        <p>A challenge of synthetic biology is repeating measurements in different laboratories. For example, fluorescence data is difficult to compare either because it is reported in different units, or because different groups handle raw data differently. iGEM’s Measurement Committee thus aims to use the InterLab Study to eventually develop absolute units for measurements of green fluorescent protein (GFP) in a plate reader. This will improve the measurement tools of synthetic biologists.
+                        <p>A challenge of synthetic biology is repeating measurements in different laboratories. For example, fluorescence data is difficult to compare either because it is reported in different units, or because different groups handle raw data differently. iGEM’s Measurement Committee thus aims to use the InterLab Study to eventually develop absolute units for measurements of green fluorescent protein (GFP) in a plate reader. This will improve the measurement tools of synthetic biologists.
-        </p>
+                        </p>
-        <p> This year, the Committee aims to discover if it is possible to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of optical density (OD). For this, we were required to measure the cell density of Escherichia coli (<i> E.Coli </i>) DH5⍺ cells using the methods below.
+                        <p> This year, the Committee aims to discover if it is possible to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of optical density (OD). For this, we were required to measure the cell density of Escherichia coli (<i> E.Coli </i>) DH5⍺ cells using the methods below.
-        </p>
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-      <td>
+                    <td>
-        <p> <i> Method 1: Converting between absorbance of cells to absorbance of a known concentration of beads </i>
+                        <p> <i> Method 1: Converting between absorbance of cells to absorbance of a known concentration of beads </i>
-        </p>
+                        </p>
-        <p>&emsp;</p>
+                        <p>&emsp;</p>
-        <p>  In the first method, silica beads are used to estimate the actual amount of cells during fluorescence measurement. These beads are modeled after a typical <i> E. coli </i> cell and are thus expected to scatter light in a similar way to <i> E. Coli </i> cells. As a sample of these silica beads gives a consistent and known absorbance measurement at 600 nm, absorbance measurements from a sample’s cell density can be converted into an “equivalent concentration of beads” measurement that should be more universal and comparable between different labs.
+                        <p>  In the first method, silica beads are used to estimate the actual amount of cells during fluorescence measurement. These beads are modeled after a typical <i> E. coli </i> cell and are thus expected to scatter light in a similar way to <i> E. Coli </i> cells. As a sample of these silica beads gives a consistent and known absorbance measurement at 600 nm, absorbance measurements from a sample’s cell density can be converted into an “equivalent concentration of beads” measurement that should be more universal and comparable between different labs.
-        </p>
+                        </p>
-        <p>&emsp;</p>
+                        <p>&emsp;</p>
-        <p> <i>Method 2: Counting colony-forming units (CFUs) from the sample</i>
+                        <p> <i>Method 2: Counting colony-forming units (CFUs) from the sample</i>
-        </p>
+                        </p>
-        <p>&emsp;</p>
+                        <p>&emsp;</p>
-        <p> In the second method, cell concentration is approximated is by plating a known volume of the sample and letting bacterial colonies grow. As each bacterial colony is assumed to represent a single cell (for cells that do not stick together), the cell concentration in the sample is then directly proportional to the number of CFUs. Using a scaling factor computed from negative and positive control CFUs, a conversion factor from absorbance to CFU can be computed.
+                        <p> In the second method, cell concentration is approximated is by plating a known volume of the sample and letting bacterial colonies grow. As each bacterial colony is assumed to represent a single cell (for cells that do not stick together), the cell concentration in the sample is then directly proportional to the number of CFUs. Using a scaling factor computed from negative and positive control CFUs, a conversion factor from absorbance to CFU can be computed.
-        </p>
+                        </p>
-      </td>
+                    </td>
-    </tr>
+                 </tr>
-  </table>
-</div>
-<div class="parts-wrapper">
-  <h2> PARTS RECEIVED </h2>
-  <table style="width:70%">
-    <tr>
-      <th><i>Device</i></th>
-      <th><i>Part Number</i></th>
-      <th><i>Usage</i></th>
-    </tr>
-    <tr>
-      <td>Negative control</td>
-      <td><a href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a></td>
-      <td>TetR repressible promoter, medium strength promoter</td>
-    </tr>
-    <tr>
-      <td>Positive Control</td>
-      <td><a href="http://parts.igem.org/Part:BBa_I20270">BBa_I20270</a></td>
-      <td>Promoter MeasKit (J23151)</td>
-    </tr>
-    <tr>
-      <td>Test Device 1</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364000">BBa_J364000</a></td>
-      <td>GFP expressing constitutive device</td>
-    </tr>
-    <tr>
-      <td>Test Device 2</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364001">BBa_J364001</a></td>
-      <td>GFP expressing constitutive device</td>
-    </tr>
-    <tr>
-      <td>Test Device 3</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364002">BBa_J364002</a></td>
-      <td>GFP expressing constitutive device</td>
-    </tr>
-    <tr>
-      <td>Test Device 4</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364007">BBa_J364007</a></td>
-      <td>Expresses GFP under the control of a constitutive promoter</td>
-    </tr>
-    <tr>
-      <td>Test Device 5</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364008">BBa_J364008</a></td>
-      <td>Expresses GFP under the control of a constitutive promoter</td>
-    </tr>
-    <tr>
-      <td>Test Device 6</td>
-      <td><a href="http://parts.igem.org/Part:BBa_J364009">BBa_J364009</a></td>
-      <td>Expresses GFP under the control of a constitutive promoter</td>
-    </tr>
-  </table>
-</div>
-<style>
-.materials{
-padding: 2em;
-text-align: center;
-}
-</style>
-<div class="materials">
-  <h2 style="padding-left: 0;"> MATERIALS & METHODS </h2>
-</div>
-<button class="accordion"> PLATE READER SETUP </button>
-<div class="panel" style="margin=auto; margin-right: 2em; text-align:center; line-height: 17em;">
-      <table style="max-width: auto; display:inline-block;">
-        <tr>
-          <td style="padding:0;">
-            <i>Abs<sub>600</sub> --</i>
-          </td>
-          <td>
-            <ul style="list-style: none; margin: 0; padding: 1em; text-align:left; border-left: .5px solid black">
-              <li> Wavelength: 600nm </li>
-              <li> Read Speed: Normal </li>
-              <li> Delay: 100 msec </li>
-            </ul>
-          </td>
-          <td>
-        </tr>
-      </table>
-      <table style="max-width: auto; margin:auto; margin-left: 2em; display: inline-block;">
-        <tr>
-          <td style="padding:0;">
-            <i>Fluorescence --</i>
-          </td>
-          <td>
-            <ul style="list-style: none; margin: 0; padding: 1em; text-align:left; border-left: .5px solid black">
-              <li> Excitation: 485 </li>
-              <li>Emission: 525</li>
-              <li>Optics: Top</li>
-              <li>Gain: 50</li>
-              <li>Light Source: Xenon Flash</li>
-              <li>Lamp Energy: High</li>
-              <li>Read Speed: Normal</li>
-              <li>Delay: 100 msec</li>
-              <li>Read Height: 7 mm</li>
-            </ul>
-          </td>
-        </tr>
-      </table>
-</div>
-<button class="accordion"> OD600 REFERENCE POINT (CALIBRATION 1)  </button>
-<div class="panel">
-    <center>
-      <table cellspacing="100" style="max-width:70%; width: 72em;">
-        <tr>
-          <td>
-            <i> Materials </i>
-            <ul style="list-style: none;">
-              <li>1 ml LUDOX CL-X</li>
-              <li>ddH<sub>2</sub>O</li>
-              <li>96-well plate (black)</li>
-            </ul>
-            <br>
-            <i> Method </i>
-            <ol>
-              <li style="padding-left: 4em;">100 µl of LUDOX was added into wells A1, B1, C1 and D1. </li>
-              <li style="padding-left: 4em;">100 µl of ddH20 was added into wells A2, B2, C2 and D2.</li>
-              <li style="padding-left: 4em;">Abs<sub>600nm</sub> was measured for all samples.</li>
-            </ol>
-          </td>
-          <td>
-            <table style="width: auto; font-size:1em;">
-              <tr>
-                 <td> <i> Results </i> </td>
-                <td style="background-color: #f2f2f2"> <i> LUDOX CL-X </i> </td>
-                <td style="background-color: #f2f2f2"> <i> H<sub>2</sub>O </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Replicate 1 </i> </td>
-                <td> 0.056 </td>
-                <td> 0.037 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Replicate 2 </i> </td>
-                <td> 0.056 </td>
-                <td> 0.038 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Replicate 3 </i> </td>
-                <td> 0.056 </td>
-                <td> 0.038 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Replicate 4 </i> </td>
-                <td> 0.055 </td>
-                <td> 0.038 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Arth. Mean </i> </td>
-                <td> 0.056 </td>
-                <td> 0.038 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Corrected Abs<sub>600</sub> </i> </td>
-                <td> 0.018 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Reference OD<sub>600</sub> </i> </td>
-                <td> 0.063 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> OD<sub>600</sub>/Abs<sub>600</sub> </i> </td>
-                <td> 0.063 </td>
-              </tr>
              </table>
-          </td>
+        </div>
-         </tr>
+        <div class="parts-wrapper">
-      </table>
+            <h2> PARTS RECEIVED </h2>
-    </center>
+            <table style="width:70%">
-</div>
+                <tr>
+                    <th><i>Device</i></th>
-<button class="accordion">PARTICLE STANDARD CURVE (CALIBRATION 2)</button>
+                    <th><i>Part Number</i></th>
-<div class="panel">
+                    <th><i>Usage</i></th>
-    <center>
+                </tr>
-      <table cellspacing="100" style="max-width:70%; width: 100em;">
+                <tr>
-        <tr style="vertical-align:top;">
+                    <td>Negative control</td>
-          <td>
+                    <td><a href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a></td>
-            <i> Materials </i>
+                    <td>TetR repressible promoter, medium strength promoter</td>
-            <ul style="list-style:none;">
+                </tr>
-              <li>300 µl Silica beads (4.7 x 108 microspheres)</li>
+                <tr>
-              <li>ddH<sub>2</sub>O</li>
+                    <td>Positive Control</td>
-              <li>96-well plate (black)</li>
+                    <td><a href="http://parts.igem.org/Part:BBa_I20270">BBa_I20270</a></td>
-            </ul>
+                    <td>Promoter MeasKit (J23151)</td>
-            <p>&emsp;</p>
+                </tr>
-            <i> Methods </i>
+                <tr>
-            <i> <u> (A) To prepare the Microsphere Stock Solution </u> </i>
+                    <td>Test Device 1</td>
-             <p>&emsp;</p>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364000">BBa_J364000</a></td>
+                    <td>GFP expressing constitutive device</td>
+                </tr>
+                <tr>
+                    <td>Test Device 2</td>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364001">BBa_J364001</a></td>
+                    <td>GFP expressing constitutive device</td>
+                </tr>
+                <tr>
+                    <td>Test Device 3</td>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364002">BBa_J364002</a></td>
+                    <td>GFP expressing constitutive device</td>
+                </tr>
+                <tr>
+                    <td>Test Device 4</td>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364007">BBa_J364007</a></td>
+                    <td>Expresses GFP under the control of a constitutive promoter</td>
+                </tr>
+                <tr>
+                    <td>Test Device 5</td>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364008">BBa_J364008</a></td>
+                    <td>Expresses GFP under the control of a constitutive promoter</td>
+                </tr>
+                <tr>
+                    <td>Test Device 6</td>
+                    <td><a href="http://parts.igem.org/Part:BBa_J364009">BBa_J364009</a></td>
+                    <td>Expresses GFP under the control of a constitutive promoter</td>
+                </tr>
+            </table>
+         </div>
+        <style>
+            .materials{
+                padding: 2em;
+                text-align: center;
+            }
+            </style>
+        <div class="materials">
+            <h2 style="padding-left: 0;"> MATERIALS & METHODS </h2>
+                </div>
+                <button class="accordion"> PLATE READER SETUP </button>
+                <div class="panel" style="margin=auto; margin-right: 2em; text-align:center; line-height: 17em;">
+                <table style="max-width: auto; display:inline-block;">
+                    <tr>
+                        <td style="padding:0;">
+                            <i>Abs<sub>600</sub> --</i>
+                        </td>
+                        <td>
+                            <ul style="list-style: none; margin: 0; padding: 1em; text-align:left; border-left: .5px solid black">
+                                <li> Wavelength: 600nm </li>
+                                <li> Read Speed: Normal </li>
+                                <li> Delay: 100 msec </li>
+                            </ul>
+                        </td>
+                        <td>
+                            </tr>
+                </table>
+                <table style="max-width: auto; margin:auto; margin-left: 2em; display: inline-block;">
+                    <tr>
+                        <td style="padding:0;">
+                            <i>Fluorescence --</i>
+                        </td>
+                        <td>
+                            <ul style="list-style: none; margin: 0; padding: 1em; text-align:left; border-left: .5px solid black">
+                                <li> Excitation: 485 </li>
+                                <li>Emission: 525</li>
+                                <li>Optics: Top</li>
+                                <li>Gain: 50</li>
+                                <li>Light Source: Xenon Flash</li>
+                                <li>Lamp Energy: High</li>
+                                <li>Read Speed: Normal</li>
+                                <li>Delay: 100 msec</li>
+                                <li>Read Height: 7 mm</li>
+                            </ul>
+                        </td>
+                    </tr>
+                </table>
+        </div>
+        <button class="accordion"> OD600 REFERENCE POINT (CALIBRATION 1)  </button>
+        <div class="panel">
+            <center>
+                <table cellspacing="100" style="max-width:70%; width: 72em;">
+                    <tr>
+                        <td>
+                            <i> Materials </i>
+                            <ul style="list-style: none;">
+                                <li>1 ml LUDOX CL-X</li>
+                                <li>ddH<sub>2</sub>O</li>
+                                <li>96-well plate (black)</li>
+                            </ul>
+                            <br>
+                            <i> Method </i>
+                            <ol>
+                                <li style="padding-left: 4em;">100 µl of LUDOX was added into wells A1, B1, C1 and D1. </li>
+                                <li style="padding-left: 4em;">100 µl of ddH20 was added into wells A2, B2, C2 and D2.</li>
+                                <li style="padding-left: 4em;">Abs<sub>600nm</sub> was measured for all samples.</li>
+                            </ol>
+                        </td>
+                        <td>
+                            <table style="width: auto; font-size:1em;">
+                                <tr>
+                                    <td> <i> Results </i> </td>
+                                    <td style="background-color: #f2f2f2"> <i> LUDOX CL-X </i> </td>
+                                    <td style="background-color: #f2f2f2"> <i> H<sub>2</sub>O </i> </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Replicate 1 </i> </td>
+                                    <td> 0.056 </td>
+                                    <td> 0.037 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Replicate 2 </i> </td>
+                                    <td> 0.056 </td>
+                                    <td> 0.038 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Replicate 3 </i> </td>
+                                    <td> 0.056 </td>
+                                    <td> 0.038 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Replicate 4 </i> </td>
+                                    <td> 0.055 </td>
+                                    <td> 0.038 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Arth. Mean </i> </td>
+                                    <td> 0.056 </td>
+                                    <td> 0.038 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Corrected Abs<sub>600</sub> </i> </td>
+                                    <td> 0.018 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> Reference OD<sub>600</sub> </i> </td>
+                                    <td> 0.063 </td>
+                                </tr>
+                                <tr>
+                                    <td style="background-color: #f2f2f2"> <i> OD<sub>600</sub>/Abs<sub>600</sub> </i> </td>
+                                    <td> 0.063 </td>
+                                </tr>
+                            </table>
+                        </td>
+                    </tr>
+                </table>
+            </center>
+        </div>
+        <button class="accordion">PARTICLE STANDARD CURVE (CALIBRATION 2)</button>
+        <div class="panel">
+            <center>
+                <table cellspacing="100" style="max-width:70%; width: 100em;">
+                    <tr style="vertical-align:top;">
+                        <td>
+                            <i> Materials </i>
+                            <ul style="list-style:none;">
+                                <li>300 µl Silica beads (4.7 x 108 microspheres)</li>
+                                <li>ddH<sub>2</sub>O</li>
+                                <li>96-well plate (black)</li>
+                            </ul>
+                            <p>&emsp;</p>
+                            <i> Methods </i>
+                            <i> <u> (A) To prepare the Microsphere Stock Solution </u> </i>
+                            <p>&emsp;</p>
+                            <ol>
+                                <li style="padding-left: 4em;">Tube labelled “Silica Beads” was vortexed vigorously for 30 s.</li>
+                                <li style="padding-left: 4em;">96 µl of microspheres was immediately pipetted into a 1.5 ml eppendorf tube.</li>
+                                <li style="padding-left: 4em;">904 µl of ddH<sub>2</sub>0 was added to the microspheres. The eppendorf was vortexed well.</li>
+                            </ol>
+                            <img src="https://static.igem.org/mediawiki/2018/3/32/T--NUS_Singapore-A--InterLab_Fig1.png" alt="Fig 1">
+                        </td>
+                        <td>
+                            <i> <u> (B) To prepare the serial dilution of microsphere </u> </i>
+                            <p>&emsp;</p>
+                            <ol>
+                                <li style="padding-left: 4em;">100 µl of ddH20 was added into wells A2, B2, C2, D2...A12, B12, C12, D12.</li>
+                                <li style="padding-left: 4em;">The microsphere stock solution was vortexed vigorously for 10 s before immediately adding 200 µl of microspheres into A1.</li>
+                                <li style="padding-left: 4em;">100 µl of microsphere stock solution was transferred from A1 to A2.</li>
+                                <li style="padding-left: 4em;">Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3.</li>
+                                <li style="padding-left: 4em;">The subsequent dilutions were prepared as illustrated on Image A (below).</li>
+                                <li style="padding-left: 4em;">Samples were re-mixed immediately before putting it in the plate reader. Fluorescence<sub>(Abs600)</sub> all samples were measured. </li>
+                                <li style="padding-left: 4em;"> Fluorescence (Abs<sub>600</sub> ) of all samples were measured. Our results are reflected by Fig. 2 and Fig. 3. </li>
+                                //add picture
+                            </ol>
+                        </td>
+                    </tr>
+                </table>
+             </center>
+        </div>
+        <button class="accordion"> FLUORESCENCE STANDARD CURVE (CALIBRATION 3)</button>
+        <div class="panel">
+            <center>
+                <table cellspacing="100" style="max-width:70%; width: 90em;">
+                    <col width="80">
+                        <col width="80">
+                            <tr style="vertical-align:top;">
+                                <td>
+                                    <i> Materials </i>
+                                    <ul style="list-style:none;">
+                                        <li>Fluorescein</li>
+                                        <li>10 ml 1X PBS pH 7.4 - 7.6</li>
+                                        <li>96-well plate (black)</li>
+                                    </ul>
+                                    <br>
+                                    <i> Methods </i>
+                                    <br>
+                                    <i> <u> (A) To prepare the fluorescein stock solution </u> </i>
+                                    <ol>
+                                        <li style="padding-left: 4em;">The fluorescein kit tube was spun down to make sure that the pellet was collected at the bottom of the tube. </li>
+                                        <li style="padding-left: 4em;">10X fluorescein stock solution (100 µM) was prepared by resuspending fluorescein in 1 ml of 1X PBS. Fluorescein was checked to be properly dissolved in PBS by checking for no more visible particulates in the pipette tip when resuspending.
+                                        </li>
+                                        <li style="padding-left: 4em;"> 10X fluorescein stock solution was diluted with 1X PBS to make a 1X fluorescein solution (10 µM): 100 µl of 10X fluorescein stock solution was mixed with 900 µl of 1X PBS. </li>
+                                    </ol>
+                                    // add picture
+                                </td>
+                                <td>
+                                    <i> <u> (B) To prepare the serial dilution of fluorescein </u> </i>
+                                    <ol>
+                                        <li style="padding-left: 4em;">100 µl of PBS was added into wells A2, B2, C2, D2...A12, B12, C12, D12.</li>
+                                        <li style="padding-left: 4em;">200 µl of 1X fluorescein stock solution was added into A1, B1, C1 and D1. </li>
+                                        <li style="padding-left: 4em;">100 µl of 1X fluorescein stock solution was transferred from A1 to A2. </li>
+                                        <li style="padding-left: 4em;">Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3. </li>
+                                        <li style="padding-left: 4em;">The subsequent dilutions were prepared as illustrated by Fig. 4. </li>
+                                        <li style="padding-left: 4em;">Fluorescence of all samples were measured, and our results are reflected by Fig. 5. </li>
+                                    </ol>
+                                    // add picture
+                                </td>
+                            </tr>
+                            </table>
+            </center>
+        </div>
+        <button class="accordion">  CELL MEASUREMENTS  </button>
+        <div class="panel">
+            <center>
+                <table cellspacing="90" style="max-width:70%; width: 120em;">
+                    <col width="80">
+                        <col width="80">
+                            <tr style="vertical-align:top;">
+                                <td>
+                                    <i> Materials </i>
+                                    <p>&emsp;</p>
+                                    <ul style="list-style: none;">
+                                        <li>Competent cells (Escherichia coli strain DH5α)</li>
+                                        <li>Luria Bertani (LB) media</li>
+                                        <li>Chloramphenicol (stock concentration 25 mg/ml dissolved in ethanol) </li>
+                                        <li>50 ml Falcon tube (wrapped with a thick layer of paper towel to block light)</li>
+                                        <li>1.5 ml eppendorf tubes</li>
+                                        <li>Incubator at 37 °C</li>
+                                        <li>Ice bucket with ice </li>
+                                        <li>96-well plate (black)</li>
+                                    </ul>
+                                    <p>&emsp;</p>
+                                    <i> Methods </i>
+                                    <i> <u> Day 1: Transforming Escherichia coli strain DH5α with devices provided in the Distribution Kit </u> </i>
+                                    <ol>
+                                        <li style="padding-left: 3em;"> Each device (powder form) was resuspended in 10 µl of ddH<sub>2</sub>O.
+                                        </li>
+                                        <li style="padding-left: 3em;"> 1 µl of each respective plasmid was transformed into 50 µl <i> E. coli </i> DH5α via electroporation.
+                                        </li>
+                                        <li style="padding-left: 3em;"> 500 µl of LB was immediately added into each tube for recovery of the transformed cells.
+                                        </li>
+                                        <li style="padding-left: 3em;"> Each sample tube was incubated with shaking at 37 °C for 30 mins before 100 µl of each sample was plated onto LB + Chlor agar plates and grown overnight at 37 °C.
+                                        </li>
+                                    </ol>
+                                    <p>&emsp;</p>
+                                    <i> <u> Day 2: Selecting Colonies and Growing Cells Overnight </u> </i>
+                                    <ol>
+                                        <li style="padding-left: 3em;"> 2 colonies were selected for being both relatively bigger and more separated from the other colonies. </li>
+                                        <li style="padding-left: 3em;"> The selected colonies were then inoculated into 5 ml of LB + Chlor and grown overnight at 37 °C at 220 rpm. </li>
+                                    </ol>
+                                </td>
+                                <td>
+                                    <i> <u> Day 3: Cell Growth, Sampling and Assay </u> </i>
+                                    <p><i> Part 1: Abs<sub>600</sub>nm and Fluorescence Measurement </i></p>
+                                    <p>&emsp;</p>
+                                    <ol>
+                                        <li style="padding-left: 3em;"> A cell stock of each overnight culture was made in glycerol for storage, in case there is a need to use them again. To make this stock, 850 µl of culture was added to 350 µl of glycerol.
+                                        </li>
+                                        <li style="padding-left: 3em;"> A 1:10 dilution of each overnight culture was made in LB + Chlor (0.5 mL culture + 4.5 mL media).
+                                        </li>
+                                        <li style="padding-left: 3em;"> Abs<sub>600</sub>nm of the 1:10 diluted cultures were measured. </li>
+                                        <li style="padding-left: 3em;"> Cultures were diluted further to a target Abs600nm of 0.02 in a final volume of 12 ml LB + Chlor in a 50 ml falcon tube that was covered with tissue paper.
+                                        </li>
+                                        <li style="padding-left: 3em;"> 500 µl of samples of the diluted cultures at 0 h were transferred into 1.5 ml eppendorf tubes labelled A and B. The tubes were placed on ice until they were ready to be laid out according to the plate diagram to measure fluorescence and Abs600. Fluorescence readings at T = 0 h are shown in Fig. 6.
+                                        </li>
+                                        <li style="padding-left: 3em;"> The rest of the cultures were incubated at 37 °C and 220 rpm for 6 hours.
+                                        </li>
+                                        <li style="padding-left: 3em;"> After the 6-hour-incubation, 500 µl of these cultures were transferred into 1.5 ml eppendorf tubes before being laid out according to the plate diagram below. The samples’ fluorescence and Abs600nm were measured again. Fluorescence readings
+                                            at T = 6 h are shown in Fig. 7.
+                                        </li>
+                                    </ol>
+                                    <br>
+                                    <i> Part 2: Colony Forming Units per 0.1 OD600 E. coli Cultures </i>
+                                    <p>&emsp;</p>
+                                    <p>Only Positive Control (BBa_I20270) cultures and Negative Control (BBa_R0040) were involved in this part.
+                                    </p>
+                                    <p>&emsp;</p>
+                                    <ol>
+                                        <li style="padding-left: 3em;">Overnight cultures were diluted 10-fold in LB + Chlor media to ensure they lay in the linear detection range of our plate reader.
+                                        </li>
+                                        <li style="padding-left: 3em;">The OD<sub>600</sub>nm of cell cultures were measured. Our results are reflected by Fig. 8.
+                                        </li>
+                                        <li style="padding-left: 3em;"> Overnight cultures were diluted to OD<sub>600</sub> = 0.1 in 1 ml of LB + Chlor media. Each culture was done in triplicate.
+                                        </li>
+                                        <li style="padding-left: 3em;"> Diluted overnight cultures were checked to ensure that OD600 = 0.1, excluding the blank measurement
+                                        </li>
+                                        <li style="padding-left: 3em;"> For each starting sample, serial dilutions were prepared as shown in Fig. 9.
+                                        </li>
+                                        <li style="padding-left: 3em;"> 100 µl of Dilutions 3, 4 and 5 were aseptically spread on LB + Chlor agar plates.
+                                        </li>
+                                        <li style="padding-left: 3em;"> The plates were incubated overnight at 37 °C. </li>
+                                        <li style="padding-left: 3em;"> Colonies on each plate were counted. Our results are reflected by Fig. 10.
+                                        </li>
+                                    </ol>
+                                </td>
+                            </tr>
+                            </table>
+                <br>
+                <i> Fluorescence Raw Readings <i>
+                    <br>
+                    <table style="width: auto; font-size:1em;">
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Hour 0 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
+                            <td> 34 </td>
+                            <td> 76 </td>
+                            <td> 361 </td>
+                            <td> 122 </td>
+                            <td> 53 </td>
+                            <td> 318 </td>
+                            <td> 289 </td>
+                            <td> 88 </td>
+                            <td> 47 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
+                            <td> 71 </td>
+                            <td> 67 </td>
+                            <td> 418 </td>
+                            <td> 131 </td>
+                            <td> 42 </td>
+                            <td> 338 </td>
+                            <td> 283 </td>
+                            <td> 57 </td>
+                            <td> 34 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
+                            <td> 52 </td>
+                            <td> 66 </td>
+                            <td> 386 </td>
+                            <td> 127 </td>
+                            <td> 44 </td>
+                            <td> 309 </td>
+                            <td> 304 </td>
+                            <td> 90 </td>
+                            <td> 27 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
+                            <td> 53 </td>
+                            <td> 101 </td>
+                            <td> 391 </td>
+                            <td> 112 </td>
+                            <td> 45 </td>
+                            <td> 367 </td>
+                            <td> 314 </td>
+                            <td> 94 </td>
+                            <td> 33 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
+                            <td> 65 </td>
+                            <td> 61 </td>
+                            <td> 357 </td>
+                            <td> 100 </td>
+                            <td> 8 </td>
+                            <td> 329 </td>
+                            <td> 277 </td>
+                            <td> 76 </td>
+                            <td> 35 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
+                            <td> 47 </td>
+                            <td> 83 </td>
+                            <td> 389 </td>
+                            <td> 144 </td>
+                            <td> 24 </td>
+                            <td> 339 </td>
+                            <td> 2278 </td>
+                            <td> 91 </td>
+                            <td> 38 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
+                            <td> 79 </td>
+                            <td> 74 </td>
+                            <td> 377 </td>
+                            <td> 138 </td>
+                            <td> 33 </td>
+                            <td> 335 </td>
+                            <td> 280 </td>
+                            <td> 85 </td>
+                            <td> 45 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
+                            <td> 61 </td>
+                            <td> 65 </td>
+                            <td> 350 </td>
+                            <td> 142 </td>
+                            <td> 45 </td>
+                            <td> 327 </td>
+                            <td> 282 </td>
+                            <td> 72 </td>
+                            <td> 31 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Hour 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
+                            <td> 36 </td>
+                            <td> 461 </td>
+                            <td> 1252 </td>
+                            <td> 920 </td>
+                            <td> 58 </td>
+                            <td> 2389 </td>
+                            <td> 338 </td>
+                            <td> 436 </td>
+                            <td> 35 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
+                            <td> 45 </td>
+                            <td> 500 </td>
+                            <td> 1351 </td>
+                            <td> 988 </td>
+                            <td> 55 </td>
+                            <td> 2621 </td>
+                            <td> 354 </td>
+                            <td> 433 </td>
+                            <td> 54 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
+                            <td> 53 </td>
+                            <td> 501 </td>
+                            <td> 1390 </td>
+                            <td> 1014 </td>
+                            <td> 60 </td>
+                            <td> 2757 </td>
+                            <td> 393 </td>
+                            <td> 446 </td>
+                            <td> 24 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
+                            <td> 61 </td>
+                            <td> 488 </td>
+                            <td> 1332 </td>
+                            <td> 1039 </td>
+                            <td> 36 </td>
+                            <td> 2793 </td>
+                            <td> 351 </td>
+                            <td> 448 </td>
+                            <td> 31 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
+                            <td> 30 </td>
+                            <td> 429 </td>
+                            <td> 971 </td>
+                            <td> 821 </td>
+                            <td> 44 </td>
+                            <td> 2142 </td>
+                            <td> 371 </td>
+                            <td> 437 </td>
+                            <td> 8 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
+                            <td> 55 </td>
+                            <td> 516 </td>
+                            <td> 1199 </td>
+                            <td> 832 </td>
+                            <td> 96 </td>
+                            <td> 2390 </td>
+                            <td> 378 </td>
+                            <td> 401 </td>
+                            <td> 24 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
+                            <td> 38 </td>
+                            <td> 483 </td>
+                            <td> 1239 </td>
+                            <td> 838 </td>
+                            <td> 74 </td>
+                            <td> 2401 </td>
+                            <td> 425 </td>
+                            <td> 423 </td>
+                            <td> 40 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
+                            <td> 49 </td>
+                            <td> 460 </td>
+                            <td> 1129 </td>
+                            <td> 896 </td>
+                            <td> 57 </td>
+                            <td> 2528 </td>
+                            <td> 384 </td>
+                            <td> 423 </td>
+                            <td> 51 </td>
+                        </tr>
+                    </table>
+                    <br>
+                    <i> OD<sub>600</sub> Raw Readings </i>
+                    <br>
+                    <table style="width: auto; font-size:1em;">
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Hour 0: </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
+                            <td> 0.061 </td>
+                            <td> 0.06 </td>
+                            <td> 0.061 </td>
+                            <td> 0.063 </td>
+                            <td> 0.059 </td>
+                            <td> 0.059 </td>
+                            <td> 0.063 </td>
+                            <td> 0.061 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
+                            <td> 0.062 </td>
+                            <td> 0.062 </td>
+                            <td> 0.063 </td>
+                            <td> 0.063 </td>
+                            <td> 0.063 </td>
+                            <td> 0.062 </td>
+                            <td> 0.065 </td>
+                            <td> 0.063 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
+                            <td> 0.063 </td>
+                            <td> 0.062 </td>
+                            <td> 0.064 </td>
+                            <td> 0.065 </td>
+                            <td> 0.064 </td>
+                            <td> 0.061 </td>
+                            <td> 0.063 </td>
+                            <td> 0.061 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
+                            <td> 0.062 </td>
+                            <td> 0.062 </td>
+                            <td> 0.065 </td>
+                            <td> 0.063 </td>
+                            <td> 0.063 </td>
+                            <td> 0.062 </td>
+                            <td> 0.063 </td>
+                            <td> 0.062 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
+                            <td> 0.06 </td>
+                            <td> 0.063 </td>
+                            <td> 0.064 </td>
+                            <td> 0.063 </td>
+                            <td> 0.062 </td>
+                            <td> 0.062 </td>
+                            <td> 0.062 </td>
+                            <td> 0.064 </td>
+                            <td> 0.045 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
+                            <td> 47 </td>
+                            <td> 83 </td>
+                            <td> 389 </td>
+                            <td> 144 </td>
+                            <td> 24 </td>
+                            <td> 339 </td>
+                            <td> 2278 </td>
+                            <td> 91 </td>
+                            <td> 38 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
+                            <td> 0.061 </td>
+                            <td> 0.063 </td>
+                            <td> 0.061 </td>
+                            <td> 0.065 </td>
+                            <td> 0.061 </td>
+                            <td> 0.065 </td>
+                            <td> 0.06 </td>
+                            <td> 0.062 </td>
+                            <td> 0.046 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
+                            <td> 0.061 </td>
+                            <td> 0.063 </td>
+                            <td> 0.061 </td>
+                            <td> 0.065 </td>
+                            <td> 0.061 </td>
+                            <td> 0.065 </td>
+                            <td> 0.06 </td>
+                            <td> 0.062 </td>
+                            <td> 0.046 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Hour 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
+                            <td> 0.428 </td>
+                            <td> 0.415 </td>
+                            <td> 0.174 </td>
+                            <td> 0.445 </td>
+                            <td> 0.455 </td>
+                            <td> 0.418 </td>
+                            <td> 0.074 </td>
+                            <td> 0.432 </td>
+                            <td> 0.042 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
+                            <td> 0.452 </td>
+                            <td> 0.435 </td>
+                            <td> 0.19 </td>
+                            <td> 0.481 </td>
+                            <td> 0.481 </td>
+                            <td> 0.46 </td>
+                            <td> 0.08 </td>
+                            <td> 0.469 </td>
+                            <td> 0.042 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
+                            <td> 0.459 </td>
+                            <td> 0.434 </td>
+                            <td> 0.198 </td>
+                            <td> 0.495 </td>
+                            <td> 0.487 </td>
+                            <td> 0.462 </td>
+                            <td> 0.081 </td>
+                            <td> 0.46 </td>
+                            <td> 0.042 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
+                            <td> 0.461 </td>
+                            <td> 0.439 </td>
+                            <td> 0.185 </td>
+                            <td> 0.495 </td>
+                            <td> 0.479 </td>
+                            <td> 0.477 </td>
+                            <td> 0.076 </td>
+                            <td> 0.467 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
+                            <td> 0.446 </td>
+                            <td> 0.414 </td>
+                            <td> 0.138 </td>
+                            <td> 0.434 </td>
+                            <td> 0.443 </td>
+                            <td> 0.406 </td>
+                            <td> 0.079 </td>
+                            <td> 0.419 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
+                            <td> 0.467 </td>
+                            <td> 0.447 </td>
+                            <td> 0.149 </td>
+                            <td> 0.452 </td>
+                            <td> 0.484 </td>
+                            <td> 0.437 </td>
+                            <td> 0.076 </td>
+                            <td> 0.435 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
+                            <td> 0.496 </td>
+                            <td> 0.448 </td>
+                            <td> 0.153 </td>
+                            <td> 0.449 </td>
+                            <td> 0.485 </td>
+                            <td> 0.455 </td>
+                            <td> 0.082 </td>
+                            <td> 0.435 </td>
+                            <td> 0.043 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
+                            <td> 0.483 </td>
+                            <td> 0.444 </td>
+                            <td> 0.148 </td>
+                            <td> 0.475 </td>
+                            <td> 0.467 </td>
+                            <td> 0.455 </td>
+                            <td> 0.079 </td>
+                            <td> 0.409 </td>
+                            <td> 0.04 </td>
+                        </tr>
+                    </table>
+                    <br>
+                    Counts for Colony-Forming Units
+                    <table style="width: auto; font-size:1em;">
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> D4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 445 </td>
+                            <td> 286 </td>
+                            <td> 649 </td>
+                            <td> 173 </td>
+                            <td> 608 </td>
+                            <td> 945 </td>
+                            <td> 4.60E + 02 </td>
+                            <td> 5.75E + 02 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 195 </td>
+                            <td> 219 </td>
+                            <td> 245 </td>
+                            <td> 232 </td>
+                            <td> 250 </td>
+                            <td> 253 </td>
+                            <td> 2.20E + 02 </td>
+                            <td> 2.45E + 02 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> D5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 49 </td>
+                            <td> 32 </td>
+                            <td> 89 </td>
+                            <td> 32 </td>
+                            <td> 153 </td>
+                            <td> 418 </td>
+                            <td> 5.67E + 01 </td>
+                            <td> 2.01E + 02 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 36 </td>
+                            <td> 54 </td>
+                            <td> 35 </td>
+                            <td> 29 </td>
+                            <td> 4 </td>
+                            <td> 27 </td>
+                            <td> 4.17E + 02 </td>
+                            <td> 2.00E + 02 </td>
+                        </tr>
+                    </table>
+                    <br>
+                    Cell Concentration
+                    <br>
+                    <table style="width: auto; font-size:1em;">
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> D4 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 3.52E + 09 </td>
+                            <td> 2.24E + 09 </td>
+                            <td> 5.14E + 09 </td>
+                            <td> 1.41E + 09 </td>
+                            <td> 4.72E + 09 </td>
+                            <td> 7.49E + 09 </td>
+                            <td> 3.64E + 09 </td>
+                            <td> 4.54E + 09 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 1.61E + 09 </td>
+                            <td> 1.95E + 09 </td>
+                            <td> 2.11E + 09 </td>
+                            <td> 1.70E + 09 </td>
+                            <td> 1.85E + 09 </td>
+                            <td> 2.25E + 09 </td>
+                            <td> 1.89E + 09 </td>
+                            <td> 1.93E + 09 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> D5 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 3.88E + 09 </td>
+                            <td> 2.51E + 09 </td>
+                            <td> 7.05E + 09 </td>
+                            <td> 2.61E + 09 </td>
+                            <td> 1.19E + 10 </td>
+                            <td> 3.31E + 10 </td>
+                            <td> 4.48E + 09 </td>
+                            <td> 1.59E + 10 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td style="background-color: #f2f2f2"> <i>         </i> </td>
+                            <td> 2.97E + 09 </td>
+                            <td> 4.80E + 09 </td>
+                            <td> 3.01E + 09 </td>
+                            <td> 2.13E + 09 </td>
+                            <td> 2.96E + 08 </td>
+                            <td> 2.40E + 09 </td>
+                            <td> 3.59E + 09 </td>
+                            <td> 1.61E + 09 </td>
+                        </tr>
+                    </table>
+                    <br>
+                    CFU/mL/OD
+                    <br>
+                    <table style="width: auto; font-size:1em;">
+                        <tr>
+                            <td>  <i>      </i> </td>
+                            <td style="background-color: #f2f2f2"> <i> Avg of D4 and D5 </i> </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> NCA </i> </td>
+                            <td> 4.06E + 09 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> NCB </i> </td>
+                            <td> 1.02E + 10 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> PCA </i> </td>
+                            <td> 2.74E + 09 </td>
+                        </tr>
+                        <tr>
+                            <td style="background-color: #f2f2f2"> <i> PCB </i> </td>
+                            <td> 1.77E + 09 </td>
+                        </tr>
+                    </table>
+                    </center>
+            </p>
+        </div>
+        <button class="accordion"> FLOW CYTOMETRY </button>
+        <div class="panel">
+            <p>
+            <center>
+                <table style="width:70%">
+                    <col width="80">
+                        <col width="80">
+                            <tr>
+                                <td>
+                                    <i> Materials </i>
+                                    <ol>
+                                        <li>SpheroTech Rainbow calibration beads, type RCP-30-5A (Lot Number: AAF02) </li>
+                                        <li>Flow cytometer set to collect 10,000 events per well or a read time of 1 min per well</li>
+                                    </ol>
+                                </td>
+                                <td>
+                                    <i> Methods </i>
+                                    <ol>
+                                        <li>A sample of SpheroTech beads was prepared according to the manufacturer instructions</li>
+                                        <li>Plate setup was as shown below.</li>
+                                    </ol>
+                                </td>
+                            </tr>
+                            </table>
+            </center>
+            </p>
+        </div>
+        <style>
+            .interlab_discussion{
+                padding: 2em;
+            }
+            </style>
+        <div class="interlab_discussion">
+            <h2 style="padding-left: 0;">DISCUSSION</h2>
+            <i> Abs<sub>600</sub> nm </i>
+            <p></p>
+            <p>Rate of growth is inferred from Net Abs 600 nm values; Net Abs 600 nm is a common method of measuring cell concentration. All cells except for cells transformed with Devices 1 and 5 had comparable rates of growth. In cells transformed with Devices 1 and 5, the increase in Net Abs 600 nm was significantly slower than cells transformed with the rest of the devices (see Figure Z).
+            //add pictures
+            </p>
+            <p></p>
+            <i> µM Fluorescein per OD </i>
+            <p></p>
              <ol>
-              <li style="padding-left: 4em;">Tube labelled “Silica Beads” was vortexed vigorously for 30 s.</li>
+                <li> Cells transformed with Devices 1, 4 and 5 had the highest fluorescein readings per OD. </li>
-              <li style="padding-left: 4em;">96 µl of microspheres was immediately pipetted into a 1.5 ml eppendorf tube.</li>
+                <li> µM fluorescein per OD of cells transformed with Device 3 were very low and at levels comparable to cells transformed with the Negative Control. </li>
-              <li style="padding-left: 4em;">904 µl of ddH<sub>2</sub>0 was added to the microspheres. The eppendorf was vortexed well.</li>
+                <li> Cells transformed with Devices 2 and 6 had similar µM fluorescein per OD to that of cells transformed with the Positive Control. </li>
+                <li> Similar trends were observed for MEFL/particle. </li>
              </ol>
-            //add picture
+             <p></p>
-          </td>
+             <center>
+                <table style="width:70%">
-          <td>
+                    <col width="80">
-            <i> <u> (B) To prepare the serial dilution of microsphere </u> </i>
+                        <col width="80">
-             <p>&emsp;</p>
+                            <tr>
-             <ol>
+                                <td>
-              <li style="padding-left: 4em;">100 µl of ddH20 was added into wells A2, B2, C2, D2...A12, B12, C12, D12.</li>
+                                    <i> Derivations/Inferences made about devices </i>
-              <li style="padding-left: 4em;">The microsphere stock solution was vortexed vigorously for 10 s before immediately adding 200 µl of microspheres into A1.</li>
+                                    <ol>
-              <li style="padding-left: 4em;">100 µl of microsphere stock solution was transferred from A1 to A2.</li>
+                                        <li> Despite growing at a considerably slower rate, fluorescein readings per OD of cells transformed with Devices 1 and 5 were among the highest. The high GFP production in cells transformed with Devices 1 and 5 might have caused a unhealthy metabolic stress on the cells, which, in turn, could have impeded the growth of the cells.
-              <li style="padding-left: 4em;">Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3.</li>
+                                        </li>
-              <li style="padding-left: 4em;">The subsequent dilutions were prepared as illustrated on Image A (below).</li>
+                                        <li> Cells transformed with Device 4 had a slightly lower fluorescein reading per OD as compared to cells transformed with Devices 1 and 5. Since there was no compromise on the cell growth rate despite the high GFP production, the slightly lower GFP production (as compared to cells transformed with Devices 1 and 5) was inferred to have permitted the cells to grow at normal rates, leading to an overall higher net fluorescein reading.
-              <li style="padding-left: 4em;">Samples were re-mixed immediately before putting it in the plate reader. Fluorescence<sub>(Abs600)</sub> all samples were measured. </li>
+                                        </li>
-              <li style="padding-left: 4em;"> Fluorescence (Abs<sub>600</sub> ) of all samples were measured. Our results are reflected by Fig. 2 and Fig. 3. </li>
+                                        <li> Cells transformed with Device 3 was observed to produce very little to no GFP. Promoter within Device 3 might have been repressed by a molecule present in the cells, or not activated, to result in minimal GFP expression.
-              //add picture
+                                        </li>
-            </ol>
+                                    </ol>
-          </td>
+                                </td>
-        </tr>
+                                <td>
-      </table>
+                                    <i> Conclusion about devices </i>
-    </center>
+                                    <p>Devices 1 and 5 were inferred to have highest promoter strength upstream of the GFP gene. While they produced the highest fluorescein readings per OD, overexpression of GFP as a result of Devices 1 and 5 do not seem to be healthy for cells: cells were not observed to be able to cope with the overly high expression of GFP. In the same argument, Device 4 seemed to be the most advantageous for the experimenter: there was a high GFP production without a compromise of growth rate. Cells transformed with Device 4 seemed to be able to manage the level of metabolic stress and grow normally.
-</div>
+                                    </p>
+                                </td>
-<button class="accordion"> FLUORESCENCE STANDARD CURVE (CALIBRATION 3)</button>
+                            </tr>
-<div class="panel">
+                            </table>
-    <center>
+             </center>
-      <table cellspacing="100" style="max-width:70%; width: 90em;">
-        <col width="80">
-        <col width="80">
-        <tr style="vertical-align:top;">
-          <td>
-            <i> Materials </i>
-            <ul style="list-style:none;">
-              <li>Fluorescein</li>
-              <li>10 ml 1X PBS pH 7.4 - 7.6</li>
-              <li>96-well plate (black)</li>
-             </ul>
              <br>
-             <i> Methods </i>
+             <center>
-            <br>
+                <table style="width:70%">
-            <i> <u> (A) To prepare the fluorescein stock solution </u> </i>
+                    <col width="80">
-            <ol>
+                        <col width="80">
-              <li style="padding-left: 4em;">The fluorescein kit tube was spun down to make sure that the pellet was collected at the bottom of the tube. </li>
+                            <tr>
-              <li style="padding-left: 4em;">10X fluorescein stock solution (100 µM) was prepared by resuspending fluorescein in 1 ml of 1X PBS. Fluorescein was checked to be properly dissolved in PBS by checking for no more visible particulates in the pipette tip when resuspending.
+                                <td>
-                </li>
+                                    <i> CFU/mL/OD </i>
-              <li style="padding-left: 4em;"> 10X fluorescein stock solution was diluted with 1X PBS to make a 1X fluorescein solution (10 µM): 100 µl of 10X fluorescein stock solution was mixed with 900 µl of 1X PBS. </li>
+                                    <br>
-            </ol>
+                                    Based on the data, NCA, NCB, PCA, PCB averages were computed based on counted CFU units. The percentage errors computed based on the known concentrations of beads against their respective averages showed high error levels.
-            // add picture
+                                    <br>
-          </td>
+                                    // Confirm with wiki team
+                                </td>
-          <td>
+                            </tr>
-            <i> <u> (B) To prepare the serial dilution of fluorescein </u> </i>
+                </table>
-            <ol>
-              <li style="padding-left: 4em;">100 µl of PBS was added into wells A2, B2, C2, D2...A12, B12, C12, D12.</li>
+                </p>
-              <li style="padding-left: 4em;">200 µl of 1X fluorescein stock solution was added into A1, B1, C1 and D1. </li>
+        </div>
-              <li style="padding-left: 4em;">100 µl of 1X fluorescein stock solution was transferred from A1 to A2. </li>
-              <li style="padding-left: 4em;">Mix A2 by pipetting up and down 3 times and transfer 100 µl into A3. </li>
-              <li style="padding-left: 4em;">The subsequent dilutions were prepared as illustrated by Fig. 4. </li>
-              <li style="padding-left: 4em;">Fluorescence of all samples were measured, and our results are reflected by Fig. 5. </li>
+         <div class="column full_size judges-will-not-evaluate">
-            </ol>
+             <h3>★  ALERT! </h3>
-            // add picture
+             <p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
-          </td>
+             <p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
-        </tr>
+        </div>
-      </table>
-    </center>
-</div>
+        <div class="clear"></div>
-<button class="accordion">  CELL MEASUREMENTS  </button>
-<div class="panel">
+        <div class="column full_size">
-    <center>
+             <h1>InterLab</h1>
-      <table cellspacing="90" style="max-width:70%; width: 120em;">
+             <h3>Bronze Medal Criterion #4</h3>
-         <col width="80">
+             <p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
-         <col width="80">
+            <br><br>
-         <tr style="vertical-align:top;">
+            For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
-          <td>
-             <i> Materials </i>
-             <p>&emsp;</p>
-            <ul style="list-style: none;">
-              <li>Competent cells (Escherichia coli strain DH5α)</li>
-              <li>Luria Bertani (LB) media</li>
-              <li>Chloramphenicol (stock concentration 25 mg/ml dissolved in ethanol) </li>
-              <li>50 ml Falcon tube (wrapped with a thick layer of paper towel to block light)</li>
-              <li>1.5 ml eppendorf tubes</li>
-              <li>Incubator at 37 °C</li>
-              <li>Ice bucket with ice </li>
-              <li>96-well plate (black)</li>
-            </ul>
-            <p>&emsp;</p>
-             <i> Methods </i>
-            <i> <u> Day 1: Transforming Escherichia coli strain DH5α with devices provided in the Distribution Kit </u> </i>
-            <ol>
-              <li style="padding-left: 3em;"> Each device (powder form) was resuspended in 10 µl of ddH<sub>2</sub>O.
-              </li>
-              <li style="padding-left: 3em;"> 1 µl of each respective plasmid was transformed into 50 µl <i> E. coli </i> DH5α via electroporation.
-              </li>
-              <li style="padding-left: 3em;"> 500 µl of LB was immediately added into each tube for recovery of the transformed cells.
-              </li>
-              <li style="padding-left: 3em;"> Each sample tube was incubated with shaking at 37 °C for 30 mins before 100 µl of each sample was plated onto LB + Chlor agar plates and grown overnight at 37 °C.
-              </li>
-            </ol>
-            <p>&emsp;</p>
-            <i> <u> Day 2: Selecting Colonies and Growing Cells Overnight </u> </i>
-            <ol>
-              <li style="padding-left: 3em;"> 2 colonies were selected for being both relatively bigger and more separated from the other colonies. </li>
-              <li style="padding-left: 3em;"> The selected colonies were then inoculated into 5 ml of LB + Chlor and grown overnight at 37 °C at 220 rpm. </li>
-             </ol>
-          </td>
-          <td>
-             <i> <u> Day 3: Cell Growth, Sampling and Assay </u> </i>
-             <p><i> Part 1: Abs<sub>600</sub>nm and Fluorescence Measurement </i></p>
-            <p>&emsp;</p>
-            <ol>
-              <li style="padding-left: 3em;"> A cell stock of each overnight culture was made in glycerol for storage, in case there is a need to use them again. To make this stock, 850 µl of culture was added to 350 µl of glycerol.
-              </li>
-              <li style="padding-left: 3em;"> A 1:10 dilution of each overnight culture was made in LB + Chlor (0.5 mL culture + 4.5 mL media).
-              </li>
-              <li style="padding-left: 3em;"> Abs<sub>600</sub>nm of the 1:10 diluted cultures were measured. </li>
-              <li style="padding-left: 3em;"> Cultures were diluted further to a target Abs600nm of 0.02 in a final volume of 12 ml LB + Chlor in a 50 ml falcon tube that was covered with tissue paper.
-              </li>
-              <li style="padding-left: 3em;"> 500 µl of samples of the diluted cultures at 0 h were transferred into 1.5 ml eppendorf tubes labelled A and B. The tubes were placed on ice until they were ready to be laid out according to the plate diagram to measure fluorescence and Abs600. Fluorescence readings at T = 0 h are shown in Fig. 6.
-              </li>
-              <li style="padding-left: 3em;"> The rest of the cultures were incubated at 37 °C and 220 rpm for 6 hours.
-              </li>
-              <li style="padding-left: 3em;"> After the 6-hour-incubation, 500 µl of these cultures were transferred into 1.5 ml eppendorf tubes before being laid out according to the plate diagram below. The samples’ fluorescence and Abs600nm were measured again. Fluorescence readings
-                at T = 6 h are shown in Fig. 7.
-              </li>
-             </ol>
-            <br>
-            <i> Part 2: Colony Forming Units per 0.1 OD600 E. coli Cultures </i>
-            <p>&emsp;</p>
-            <p>Only Positive Control (BBa_I20270) cultures and Negative Control (BBa_R0040) were involved in this part.
              </p>
-            <p>&emsp;</p>
+         </div>
-            <ol>
+     </div>
-              <li style="padding-left: 3em;">Overnight cultures were diluted 10-fold in LB + Chlor media to ensure they lay in the linear detection range of our plate reader.
-              </li>
-              <li style="padding-left: 3em;">The OD<sub>600</sub>nm of cell cultures were measured. Our results are reflected by Fig. 8.
+     <script>
-              </li>
+         /***************************************************JAVASCRIPT STARTS HERE**************************************************/
-              <li style="padding-left: 3em;"> Overnight cultures were diluted to OD<sub>600</sub> = 0.1 in 1 ml of LB + Chlor media. Each culture was done in triplicate.
-              </li>
-              <li style="padding-left: 3em;"> Diluted overnight cultures were checked to ensure that OD600 = 0.1, excluding the blank measurement
-              </li>
-              <li style="padding-left: 3em;"> For each starting sample, serial dilutions were prepared as shown in Fig. 9.
+        var acc = document.getElementsByClassName("accordion");
-              </li>
+        var i;
-              <li style="padding-left: 3em;"> 100 µl of Dilutions 3, 4 and 5 were aseptically spread on LB + Chlor agar plates.
-              </li>
+        for (i = 0; i < acc.length; i++) {
-              <li style="padding-left: 3em;"> The plates were incubated overnight at 37 °C. </li>
+            acc[i].addEventListener("click", function() {
-              <li style="padding-left: 3em;"> Colonies on each plate were counted. Our results are reflected by Fig. 10.
-              </li>
+                                    /* Toggle between adding and removing the "active" class,
-            </ol>
+                                     to highlight the button that controls the panel */
-          </td>
-         </tr>
+                                    this.classList.toggle("active");
-      </table>
-     <br>
+                                    /* Toggle between hiding and showing the active panel */
-     <i> Fluorescence Raw Readings <i>
-     <br>
+                                    var panel = this.nextElementSibling;
+                                    if (panel.style.display === "block") {
-<table style="width: auto; font-size:1em;">
+                                    panel.style.display = "none";
-              <tr>
+                                    } else {
-                <td style="background-color: #f2f2f2"> <i> Hour 0 </i> </td>
+                                    panel.style.display = "block";
-                <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
+                                    }
-                <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
+                                    });
-                <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
-                <td> 34 </td>
-                <td> 76 </td>
-                <td> 361 </td>
-                <td> 122 </td>
-                <td> 53 </td>
-                <td> 318 </td>
-                <td> 289 </td>
-                <td> 88 </td>
-                <td> 47 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
-                <td> 71 </td>
-                <td> 67 </td>
-                <td> 418 </td>
-                <td> 131 </td>
-                <td> 42 </td>
-                <td> 338 </td>
-                <td> 283 </td>
-                <td> 57 </td>
-                <td> 34 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
-                <td> 52 </td>
-                <td> 66 </td>
-                <td> 386 </td>
-                <td> 127 </td>
-                <td> 44 </td>
-                <td> 309 </td>
-                <td> 304 </td>
-                <td> 90 </td>
-                <td> 27 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
-                <td> 53 </td>
-                <td> 101 </td>
-                <td> 391 </td>
-                <td> 112 </td>
-                <td> 45 </td>
-                <td> 367 </td>
-                <td> 314 </td>
-                <td> 94 </td>
-                <td> 33 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
-                <td> 65 </td>
-                <td> 61 </td>
-                <td> 357 </td>
-                <td> 100 </td>
-                <td> 8 </td>
-                <td> 329 </td>
-                <td> 277 </td>
-                <td> 76 </td>
-                <td> 35 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
-                <td> 47 </td>
-                <td> 83 </td>
-                <td> 389 </td>
-                <td> 144 </td>
-                <td> 24 </td>
-                <td> 339 </td>
-                <td> 2278 </td>
-                <td> 91 </td>
-                <td> 38 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
-                <td> 79 </td>
-                <td> 74 </td>
-                <td> 377 </td>
-                <td> 138 </td>
-                <td> 33 </td>
-                <td> 335 </td>
-                <td> 280 </td>
-                <td> 85 </td>
-                <td> 45 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
-                <td> 61 </td>
-                <td> 65 </td>
-                <td> 350 </td>
-                <td> 142 </td>
-                <td> 45 </td>
-                <td> 327 </td>
-                <td> 282 </td>
-                <td> 72 </td>
-                <td> 31 </td>
-              </tr>
-<tr>
-                <td style="background-color: #f2f2f2"> <i> Hour 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
-                <td> 36 </td>
-                <td> 461 </td>
-                <td> 1252 </td>
-                <td> 920 </td>
-                <td> 58 </td>
-                <td> 2389 </td>
-                <td> 338 </td>
-                <td> 436 </td>
-                <td> 35 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
-                <td> 45 </td>
-                <td> 500 </td>
-                <td> 1351 </td>
-                <td> 988 </td>
-                <td> 55 </td>
-                <td> 2621 </td>
-                <td> 354 </td>
-                <td> 433 </td>
-                <td> 54 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
-                <td> 53 </td>
-                <td> 501 </td>
-                <td> 1390 </td>
-                <td> 1014 </td>
-                <td> 60 </td>
-                <td> 2757 </td>
-                <td> 393 </td>
-                <td> 446 </td>
-                <td> 24 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
-                <td> 61 </td>
-                <td> 488 </td>
-                <td> 1332 </td>
-                <td> 1039 </td>
-                <td> 36 </td>
-                <td> 2793 </td>
-                <td> 351 </td>
-                <td> 448 </td>
-                <td> 31 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
-                <td> 30 </td>
-                <td> 429 </td>
-                <td> 971 </td>
-                <td> 821 </td>
-                <td> 44 </td>
-                <td> 2142 </td>
-                <td> 371 </td>
-                <td> 437 </td>
-                <td> 8 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
-                <td> 55 </td>
-                <td> 516 </td>
-                <td> 1199 </td>
-                <td> 832 </td>
-                <td> 96 </td>
-                <td> 2390 </td>
-                <td> 378 </td>
-                <td> 401 </td>
-                <td> 24 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
-                <td> 38 </td>
-                <td> 483 </td>
-                <td> 1239 </td>
-                <td> 838 </td>
-                <td> 74 </td>
-                <td> 2401 </td>
-                <td> 425 </td>
-                <td> 423 </td>
-                <td> 40 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
-                <td> 49 </td>
-                <td> 460 </td>
-                <td> 1129 </td>
-                <td> 896 </td>
-                <td> 57 </td>
-                <td> 2528 </td>
-                <td> 384 </td>
-                <td> 423 </td>
-                <td> 51 </td>
-              </tr>
-           </table>
-<br>
-              <i> OD<sub>600</sub> Raw Readings </i>
-<br>
-<table style="width: auto; font-size:1em;">
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Hour 0: </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
-                <td> 0.061 </td>
-                <td> 0.06 </td>
-                <td> 0.061 </td>
-                <td> 0.063 </td>
-                <td> 0.059 </td>
-                <td> 0.059 </td>
-                <td> 0.063 </td>
-                <td> 0.061 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
-                <td> 0.062 </td>
-                <td> 0.062 </td>
-                <td> 0.063 </td>
-                <td> 0.063 </td>
-                <td> 0.063 </td>
-                <td> 0.062 </td>
-                <td> 0.065 </td>
-                <td> 0.063 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
-                <td> 0.063 </td>
-                <td> 0.062 </td>
-                <td> 0.064 </td>
-                <td> 0.065 </td>
-                <td> 0.064 </td>
-                <td> 0.061 </td>
-                <td> 0.063 </td>
-                <td> 0.061 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
-                <td> 0.062 </td>
-                <td> 0.062 </td>
-                <td> 0.065 </td>
-                <td> 0.063 </td>
-                <td> 0.063 </td>
-                <td> 0.062 </td>
-                <td> 0.063 </td>
-                <td> 0.062 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
-                <td> 0.06 </td>
-                <td> 0.063 </td>
-                <td> 0.064 </td>
-                <td> 0.063 </td>
-                <td> 0.062 </td>
-                <td> 0.062 </td>
-                <td> 0.062 </td>
-                <td> 0.064 </td>
-                <td> 0.045 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
-                <td> 47 </td>
-                <td> 83 </td>
-                <td> 389 </td>
-                <td> 144 </td>
-                <td> 24 </td>
-                <td> 339 </td>
-                <td> 2278 </td>
-                <td> 91 </td>
-                <td> 38 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
-                <td> 0.061 </td>
-                <td> 0.063 </td>
-                <td> 0.061 </td>
-                <td> 0.065 </td>
-                <td> 0.061 </td>
-                <td> 0.065 </td>
-                <td> 0.06 </td>
-                <td> 0.062 </td>
-                <td> 0.046 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
-                <td> 0.061 </td>
-                <td> 0.063 </td>
-                <td> 0.061 </td>
-                <td> 0.065 </td>
-                <td> 0.061 </td>
-                <td> 0.065 </td>
-                <td> 0.06 </td>
-                <td> 0.062 </td>
-                <td> 0.046 </td>
-              </tr>
-<tr>
-                <td style="background-color: #f2f2f2"> <i> Hour 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Nge. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Pos. Control </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Device 6 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> LB + Chlor (blank) </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 1 </i> </td>
-                <td> 0.428 </td>
-                <td> 0.415 </td>
-                <td> 0.174 </td>
-                <td> 0.445 </td>
-                <td> 0.455 </td>
-                <td> 0.418 </td>
-                <td> 0.074 </td>
-                <td> 0.432 </td>
-                <td> 0.042 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 2 </i> </td>
-                <td> 0.452 </td>
-                <td> 0.435 </td>
-                <td> 0.19 </td>
-                <td> 0.481 </td>
-                <td> 0.481 </td>
-                <td> 0.46 </td>
-                <td> 0.08 </td>
-                <td> 0.469 </td>
-                <td> 0.042 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1, Replicate 3 </i> </td>
-                <td> 0.459 </td>
-                <td> 0.434 </td>
-                <td> 0.198 </td>
-                <td> 0.495 </td>
-                <td> 0.487 </td>
-                <td> 0.462 </td>
-                <td> 0.081 </td>
-                <td> 0.46 </td>
-                <td> 0.042 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 1,  Replicate 4 </i> </td>
-                <td> 0.461 </td>
-                <td> 0.439 </td>
-                <td> 0.185 </td>
-                <td> 0.495 </td>
-                <td> 0.479 </td>
-                <td> 0.477 </td>
-                <td> 0.076 </td>
-                <td> 0.467 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Repplicate 1 </i> </td>
-                <td> 0.446 </td>
-                <td> 0.414 </td>
-                <td> 0.138 </td>
-                <td> 0.434 </td>
-                <td> 0.443 </td>
-                <td> 0.406 </td>
-                <td> 0.079 </td>
-                <td> 0.419 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 2</i> </td>
-                <td> 0.467 </td>
-                <td> 0.447 </td>
-                <td> 0.149 </td>
-                <td> 0.452 </td>
-                <td> 0.484 </td>
-                <td> 0.437 </td>
-                <td> 0.076 </td>
-                <td> 0.435 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 3 </i> </td>
-                <td> 0.496 </td>
-                <td> 0.448 </td>
-                <td> 0.153 </td>
-                <td> 0.449 </td>
-                <td> 0.485 </td>
-                <td> 0.455 </td>
-                <td> 0.082 </td>
-                <td> 0.435 </td>
-                <td> 0.043 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> Colony 2, Replicate 4 </i> </td>
-                <td> 0.483 </td>
-                <td> 0.444 </td>
-                <td> 0.148 </td>
-                <td> 0.475 </td>
-                <td> 0.467 </td>
-                <td> 0.455 </td>
-                <td> 0.079 </td>
-                <td> 0.409 </td>
-                <td> 0.04 </td>
-              </tr>
-           </table>
-<br>
-Counts for Colony-Forming Units
-<table style="width: auto; font-size:1em;">
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> D4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 445 </td>
-                <td> 286 </td>
-                <td> 649 </td>
-                <td> 173 </td>
-                <td> 608 </td>
-                <td> 945 </td>
-                <td> 4.60E + 02 </td>
-                <td> 5.75E + 02 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 195 </td>
-                <td> 219 </td>
-                <td> 245 </td>
-                <td> 232 </td>
-                <td> 250 </td>
-                <td> 253 </td>
-                <td> 2.20E + 02 </td>
-                <td> 2.45E + 02 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> D5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 49 </td>
-                <td> 32 </td>
-                <td> 89 </td>
-                <td> 32 </td>
-                <td> 153 </td>
-                <td> 418 </td>
-                <td> 5.67E + 01 </td>
-                <td> 2.01E + 02 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 36 </td>
-                <td> 54 </td>
-                <td> 35 </td>
-                <td> 29 </td>
-                <td> 4 </td>
-                <td> 27 </td>
-                <td> 4.17E + 02 </td>
-                <td> 2.00E + 02 </td>
-              </tr>
-            </table>
-<br>
-Cell Concentration
-<br>
-<table style="width: auto; font-size:1em;">
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> D4 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 3.52E + 09 </td>
-                <td> 2.24E + 09 </td>
-                <td> 5.14E + 09 </td>
-                <td> 1.41E + 09 </td>
-                <td> 4.72E + 09 </td>
-                <td> 7.49E + 09 </td>
-                <td> 3.64E + 09 </td>
-                <td> 4.54E + 09 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 1.61E + 09 </td>
-                <td> 1.95E + 09 </td>
-                <td> 2.11E + 09 </td>
-                <td> 1.70E + 09 </td>
-                <td> 1.85E + 09 </td>
-                <td> 2.25E + 09 </td>
-                <td> 1.89E + 09 </td>
-                <td> 1.93E + 09 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i> D5 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> 8 x 10<sup>5</sup> </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> NCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg NCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 3.88E + 09 </td>
-                <td> 2.51E + 09 </td>
-                <td> 7.05E + 09 </td>
-                <td> 2.61E + 09 </td>
-                <td> 1.19E + 10 </td>
-                <td> 3.31E + 10 </td>
-                <td> 4.48E + 09 </td>
-                <td> 1.59E + 10 </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCAI </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCA3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB1 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB2 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> PCB3 </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCA </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg PCB </i> </td>
-              </tr>
-              <tr>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td style="background-color: #f2f2f2"> <i>         </i> </td>
-                <td> 2.97E + 09 </td>
-                <td> 4.80E + 09 </td>
-                <td> 3.01E + 09 </td>
-                <td> 2.13E + 09 </td>
-                <td> 2.96E + 08 </td>
-                <td> 2.40E + 09 </td>
-                <td> 3.59E + 09 </td>
-                <td> 1.61E + 09 </td>
-              </tr>
-              </table>
-<br>
-CFU/mL/OD
-<br>
- <table style="width: auto; font-size:1em;">
-              <tr>
-                <td>  <i>      </i> </td>
-                <td style="background-color: #f2f2f2"> <i> Avg of D4 and D5 </i> </td>
-              </tr>
-              <tr>
-               <td style="background-color: #f2f2f2"> <i> NCA </i> </td>
-               <td> 4.06E + 09 </td>
-              </tr>
-             <tr>
-               <td style="background-color: #f2f2f2"> <i> NCB </i> </td>
-               <td> 1.02E + 10 </td>
-              </tr>
-            <tr>
-               <td style="background-color: #f2f2f2"> <i> PCA </i> </td>
-               <td> 2.74E + 09 </td>
-              </tr>
-           <tr>
-               <td style="background-color: #f2f2f2"> <i> PCB </i> </td>
-               <td> 1.77E + 09 </td>
-              </tr>
-    </table>
-    </center>
-  </p>
-</div>
-<button class="accordion"> FLOW CYTOMETRY </button>
-<div class="panel">
-  <p>
-     <center>
-      <table style="width:70%">
-        <col width="80">
-        <col width="80">
-        <tr>
-          <td>
-            <i> Materials </i>
-            <ol>
-              <li>SpheroTech Rainbow calibration beads, type RCP-30-5A (Lot Number: AAF02) </li>
-              <li>Flow cytometer set to collect 10,000 events per well or a read time of 1 min per well</li>
-            </ol>
-          </td>
-          <td>
-            <i> Methods </i>
-            <ol>
-              <li>A sample of SpheroTech beads was prepared according to the manufacturer instructions</li>
-              <li>Plate setup was as shown below.</li>
-            </ol>
-          </td>
-        </tr>
-      </table>
-    </center>
-  </p>
-</div>
-<style>
-.interlab_discussion{
-padding: 2em;
-}
-</style>
-<div class="interlab_discussion">
-  <h2 style="padding-left: 0;">DISCUSSION</h2>
-  <i> Abs<sub>600</sub> nm </i>
-  <p></p>
-  <p>Rate of growth is inferred from Net Abs 600 nm values; Net Abs 600 nm is a common method of measuring cell concentration. All cells except for cells transformed with Devices 1 and 5 had comparable rates of growth. In cells transformed with Devices 1 and 5, the increase in Net Abs 600 nm was significantly slower than cells transformed with the rest of the devices (see Figure Z).
-//add pictures
-  </p>
-  <p></p>
-  <i> µM Fluorescein per OD </i>
-  <p></p>
-  <ol>
-     <li> Cells transformed with Devices 1, 4 and 5 had the highest fluorescein readings per OD. </li>
-     <li> µM fluorescein per OD of cells transformed with Device 3 were very low and at levels comparable to cells transformed with the Negative Control. </li>
-     <li> Cells transformed with Devices 2 and 6 had similar µM fluorescein per OD to that of cells transformed with the Positive Control. </li>
-    <li> Similar trends were observed for MEFL/particle. </li>
-  </ol>
-  <p></p>
-  <center>
-  <table style="width:70%">
-    <col width="80">
-    <col width="80">
-    <tr>
-      <td>
-         <i> Derivations/Inferences made about devices </i>
-        <ol>
-          <li> Despite growing at a considerably slower rate, fluorescein readings per OD of cells transformed with Devices 1 and 5 were among the highest. The high GFP production in cells transformed with Devices 1 and 5 might have caused a unhealthy metabolic stress on the cells, which, in turn, could have impeded the growth of the cells.
-          </li>
-          <li> Cells transformed with Device 4 had a slightly lower fluorescein reading per OD as compared to cells transformed with Devices 1 and 5. Since there was no compromise on the cell growth rate despite the high GFP production, the slightly lower GFP production (as compared to cells transformed with Devices 1 and 5) was inferred to have permitted the cells to grow at normal rates, leading to an overall higher net fluorescein reading.
-          </li>
-          <li> Cells transformed with Device 3 was observed to produce very little to no GFP. Promoter within Device 3 might have been repressed by a molecule present in the cells, or not activated, to result in minimal GFP expression.
-          </li>
-        </ol>
-      </td>
-      <td>
-        <i> Conclusion about devices </i>
-        <p>Devices 1 and 5 were inferred to have highest promoter strength upstream of the GFP gene. While they produced the highest fluorescein readings per OD, overexpression of GFP as a result of Devices 1 and 5 do not seem to be healthy for cells: cells were not observed to be able to cope with the overly high expression of GFP. In the same argument, Device 4 seemed to be the most advantageous for the experimenter: there was a high GFP production without a compromise of growth rate. Cells transformed with Device 4 seemed to be able to manage the level of metabolic stress and grow normally.
-        </p>
-      </td>
-    </tr>
-  </table>
-</center>
-<br>
-<center>
-<table style="width:70%">
-<col width="80">
-<col width="80">
-<tr>
-<td>
-<i> CFU/mL/OD </i>
-<br>
-Based on the data, NCA, NCB, PCA, PCB averages were computed based on counted CFU units. The percentage errors computed based on the known concentrations of beads against their respective averages showed high error levels.
-<br>
-// Confirm with wiki team
-</td>
-</tr>
-</table>
-</p>
-</div>
-<div class="column full_size judges-will-not-evaluate">
-<h3>★  ALERT! </h3>
-<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
-<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
-</div>
-<div class="clear"></div>
-<div class="column full_size">
-<h1>InterLab</h1>
-<h3>Bronze Medal Criterion #4</h3>
-<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
-<br><br>
-For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
-</p>
-</div>
-</div>
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Hour 0	Nge. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	34	76	361	122	53	318	289	88	47
Colony 1, Replicate 2	71	67	418	131	42	338	283	57	34
Colony 1, Replicate 3	52	66	386	127	44	309	304	90	27
Colony 1, Replicate 4	53	101	391	112	45	367	314	94	33
Colony 2, Repplicate 1	65	61	357	100	8	329	277	76	35
Colony 2, Replicate 2	47	83	389	144	24	339	2278	91	38
Colony 2, Replicate 3	79	74	377	138	33	335	280	85	45
Colony 2, Replicate 4	61	65	350	142	45	327	282	72	31
Hour 6	Nge. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	36	461	1252	920	58	2389	338	436	35
Colony 1, Replicate 2	45	500	1351	988	55	2621	354	433	54
Colony 1, Replicate 3	53	501	1390	1014	60	2757	393	446	24
Colony 1, Replicate 4	61	488	1332	1039	36	2793	351	448	31
Colony 2, Repplicate 1	30	429	971	821	44	2142	371	437	8
Colony 2, Replicate 2	55	516	1199	832	96	2390	378	401	24
Colony 2, Replicate 3	38	483	1239	838	74	2401	425	423	40
Colony 2, Replicate 4	49	460	1129	896	57	2528	384	423	51

Hour 0:	Nge. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	0.061	0.06	0.061	0.063	0.059	0.059	0.063	0.061	0.043
Colony 1, Replicate 2	0.062	0.062	0.063	0.063	0.063	0.062	0.065	0.063	0.043
Colony 1, Replicate 3	0.063	0.062	0.064	0.065	0.064	0.061	0.063	0.061	0.043
Colony 1, Replicate 4	0.062	0.062	0.065	0.063	0.063	0.062	0.063	0.062	0.043
Colony 2, Repplicate 1	0.06	0.063	0.064	0.063	0.062	0.062	0.062	0.064	0.045
Colony 2, Replicate 2	47	83	389	144	24	339	2278	91	38
Colony 2, Replicate 3	0.061	0.063	0.061	0.065	0.061	0.065	0.06	0.062	0.046
Colony 2, Replicate 4	0.061	0.063	0.061	0.065	0.061	0.065	0.06	0.062	0.046
Hour 6	Nge. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	0.428	0.415	0.174	0.445	0.455	0.418	0.074	0.432	0.042
Colony 1, Replicate 2	0.452	0.435	0.19	0.481	0.481	0.46	0.08	0.469	0.042
Colony 1, Replicate 3	0.459	0.434	0.198	0.495	0.487	0.462	0.081	0.46	0.042
Colony 1, Replicate 4	0.461	0.439	0.185	0.495	0.479	0.477	0.076	0.467	0.043
Colony 2, Repplicate 1	0.446	0.414	0.138	0.434	0.443	0.406	0.079	0.419	0.043
Colony 2, Replicate 2	0.467	0.447	0.149	0.452	0.484	0.437	0.076	0.435	0.043
Colony 2, Replicate 3	0.496	0.448	0.153	0.449	0.485	0.455	0.082	0.435	0.043
Colony 2, Replicate 4	0.483	0.444	0.148	0.475	0.467	0.455	0.079	0.409	0.04

D4	8 x 10⁵	NCAI	NCA2	NCA3	NCB1	NCB2	NCB3	Avg NCA	Avg NCB
		445	286	649	173	608	945	4.60E + 02	5.75E + 02
		PCAI	PCA2	PCA3	PCB1	PCB2	PCB3	Avg PCA	Avg PCB
		195	219	245	232	250	253	2.20E + 02	2.45E + 02
D5	8 x 10⁵	NCAI	NCA2	NCA3	NCB1	NCB2	NCB3	Avg NCA	Avg NCB
		49	32	89	32	153	418	5.67E + 01	2.01E + 02
		PCAI	PCA2	PCA3	PCB1	PCB2	PCB3	Avg PCA	Avg PCB
		36	54	35	29	4	27	4.17E + 02	2.00E + 02

	Avg of D4 and D5
NCA	4.06E + 09
NCB	1.02E + 10
PCA	2.74E + 09
PCB	1.77E + 09

Difference between revisions of "Team:NUS Singapore-A/InterLab"

Revision as of 10:26, 6 July 2018

Interlab Study

OVERVIEW

PARTS RECEIVED

MATERIALS & METHODS

DISCUSSION

★ ALERT!

InterLab

Bronze Medal Criterion #4