Difference between revisions of "Team:RHIT/InterLab"
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Revision as of 14:57, 31 July 2018
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Callibration
Before starting the experiments, our team calibrated the necessary equipment we would need for the InterLab study.
We calibrated the plate reader using serial dilutions of fluorescein. The table to the right represents the plate wells, and all values are measured in μM.
The table to the left shows our microsphere calibration measured at 630 nm.
The table to the right shows our absorbance calibration measured at 630nm. All values are measured in abs.
CFU Data
The following table represents our data from the CFU portion of InterLab. Samples of cells with an OD600 of ~0.1 were diluted and grown on LB + chloramphenicol plates overnight. The next morning, the colonies were counted and used to predict the number of colony forming units in 1 mL of media.
The graph to the left plots the absorbance of each sample in the plate reader against its calculated CFU/mL value after plating the different dilutions. We noticed a general positive correlation between the variables, which makes sense considering a greater number of cells should have a higher absorbance value. We noticed more variability in the positive cultures than the negative cultures. The 3 positive cultures in the bottom left portion of the data are all samples from the first positive culture and the 3 in the top right portion of the data are the three samples from the second positive culture.