Difference between revisions of "Team:DTU-Denmark/InterLab"

Line 21: Line 21:
 
<div class="col-xs-12">
 
<div class="col-xs-12">
 
<h1>InterLab</h1>
 
<h1>InterLab</h1>
 
+
<h2>This page (like the others) will be improved.</h2>
 
</div>
 
</div>
  
Line 56: Line 56:
  
 
<div class="interlabspace ">
 
<div class="interlabspace ">
<h4 id="il226">Friday 22/6/2018</h4>
+
<h4 id="il266">Tuesday 26/6/2018</h4>
 
<hr>
 
<hr>
 
<p>
 
<p>
Today we transformed our prepared competent cells by using the <a target="_blank" href="http://parts.igem.org/Help:Protocols/Transformation">iGem 2018 interlab study transformation</a>.</p>
+
Luquid LB media was prepared using the "Luria-Bertani (LB) Medium Preparation" protocol for the interlab studies in the
 +
morning, as we did not have enough for the next step. Interlab is put on hold until we have a platereader.</p>
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
<div class="interlabspace ">
 +
<h4 id="il27">Monday 2/7/2018</h4>
 +
<hr>
 +
<p>
 +
We picked 2 colonies from each of our transformation plates and inoculated them in the apropriate LB and
 +
Chloramphenicol medium. The cells were left to grow overnight at 37 degrees Celsius.</p>
 +
 
 +
</div>
 +
 
 +
 
 +
<div class="interlabspace ">
 +
<h4 id="il37">Tuesday 3/7/2018</h4>
 +
<hr>
 +
<p>
 +
Today we finally had the platereader available and continued on the protocol from the 21st of June. We encountered som problems with our fluorescein samples and decided to redo them. We also decided to redo the overnight
 +
cultures as we would not have the time to use them today</p>
 +
</div>
 +
 
 +
<div class="interlabspace ">
 +
<h4 id="il47">Wednesday 4/7/2018</h4>
 +
<hr>
 +
<p>
 +
As we finally had all our controls finished, we cpntinued with the "Cell measurement" protocol. Our initial absorbance for
 +
our samples were way too high. We then finalized this part of the study and continued with the "Colony Forming Units" protocol.</p>
 +
 
 +
</div>
 +
 
 +
 
 +
<div class="interlabspace ">
 +
<h4 id="il57">Thursday5/7/2018</h4>
 +
<hr>
 +
<p>
 +
Data was extracted from our platereader and this, together with the CFU data, was sent off to finalize the interlab study.
 +
Another thing on our long list that can be ticked off.</p>
  
 
</div>
 
</div>

Revision as of 11:34, 3 August 2018

InterLab

This page (like the others) will be improved.

Thursday 21/6/2018


Competent cells were prepared for the interlab studies using the given the iGem 2018 interlab platereader protocol. We did not need to create our own competent cells as we already had high-efficiency dh5α cells ready.

Friday 22/6/2018


Today we transformed our prepared competent cells by using the iGem 2018 interlab study transformation.

Tuesday 26/6/2018


Luquid LB media was prepared using the "Luria-Bertani (LB) Medium Preparation" protocol for the interlab studies in the morning, as we did not have enough for the next step. Interlab is put on hold until we have a platereader.

Monday 2/7/2018


We picked 2 colonies from each of our transformation plates and inoculated them in the apropriate LB and Chloramphenicol medium. The cells were left to grow overnight at 37 degrees Celsius.

Tuesday 3/7/2018


Today we finally had the platereader available and continued on the protocol from the 21st of June. We encountered som problems with our fluorescein samples and decided to redo them. We also decided to redo the overnight cultures as we would not have the time to use them today

Wednesday 4/7/2018


As we finally had all our controls finished, we cpntinued with the "Cell measurement" protocol. Our initial absorbance for our samples were way too high. We then finalized this part of the study and continued with the "Colony Forming Units" protocol.

Thursday5/7/2018


Data was extracted from our platereader and this, together with the CFU data, was sent off to finalize the interlab study. Another thing on our long list that can be ticked off.