All media was prepared before 11 am to be autoclaved. The actual weights of the different media was:

All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fume hood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.