Difference between revisions of "Team:FJNU-China/InterLab"
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| + | <p>We followed this <a href="#">protocol</a> to do the Interlab Study.</br>When we completed three of the calibration measurements, performing the cell measurements. Used the same plates, volumes and settings that we used in calibration protocol. We transformed E.coli DH5α competent cells with the 8 plasmids and picked 2 colonies from each of plates into 5 mL LB medium + Chloramphenicol. After culturing the cells overnight at 37°C and 220 rpm, we used plate reader to measure the Abs600 and fluorescence of samples at 0, 6 hours.</p> | ||
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<h2 >Analyse</h2> | <h2 >Analyse</h2> | ||
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Revision as of 06:57, 30 September 2018
Interlab
Overview
Reliable and repeatable measurement is a key component to all engineering disciplines. However, the number of cells in the sample is a variability in measurements. The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements. This year, our team take part in the fifth InterLab study, using normalizing to absolute cell count or CFUs instead of OD to reduce variability of fluorescence measurements.
Materials
Strain usedEscherichia coli DH5α
Plasmid usedNegative control: BBa_R0040 Plate 7 Well 2D
Positive control: BBa_I20270 Plate 7 Well 2B
Test Device 1: BBa_J364000 Plate 7 Well 2F
Test Device 2: BBa_J364001 Plate 7 Well 2H
Test Device 3: BBa_J364002 Plate 7 Well 2J
Test Device 4: BBa_J364007 Plate 7 Well 2L
Test Device 5: BBa_J364008 Plate 7 Well 2N
Test Device 6: BBa_J364009 Plate 7 Well 2P
MachineMolecular Devices SpectraMax i3x
Methods
We followed this protocol to do the Interlab Study.When we completed three of the calibration measurements, performing the cell measurements. Used the same plates, volumes and settings that we used in calibration protocol. We transformed E.coli DH5α competent cells with the 8 plasmids and picked 2 colonies from each of plates into 5 mL LB medium + Chloramphenicol. After culturing the cells overnight at 37°C and 220 rpm, we used plate reader to measure the Abs600 and fluorescence of samples at 0, 6 hours.
Result
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Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Analyse
Suspendisse a orci facilisis, dignissim tortor vitae, ultrices mi. Vestibulum a iaculis lacus. Phasellus vitae convallis ligula, nec volutpat tellus. Vivamus scelerisque mollis nisl, nec vehicula elit egestas a. Sed luctus metus id mi gravida, faucibus convallis neque pretium. Maecenas quis sapien ut leo fringilla tempor vitae sit amet leo. Donec imperdiet tempus placerat. Pellentesque pulvinar ultrices nunc sed ultrices. Morbi vel mi pretium, fermentum lacus et, viverra tellus. Phasellus sodales libero nec dui convallis, sit amet fermentum sapien auctor. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Sed eu elementum nibh, quis varius libero.