Our team organized a laboratory safety test, everyone read and learnt carefully to apply and utilize the safety protocols, and preparing our work surroundings, which were ought to be carried out at our lab`s level of Biosafety.

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Safe lab work

Which organisms we use?

Our chassis organism is Escherichia coli. The odorless host (the tnaA gene deletion strain of Escherichia coli) is widely applied in our experiment. These organisms are commonly used with safety. And we also use Staphylococcus epidermidis in the experiment.

Although S. epidermidis is part of the normal skin flora and not usually pathogenic, patients with compromised immune systems are at risk of developing infection.

What we do with our organisms?

Our bacteria will be engineered to express a series of enzyme. Such as Escherichia coli atoDA and Clostridium acetobutylicum adhE2 were co-expressed in our host to transform E-3M2H to its correspondent alcohols. Additionally, phenyllactic acid (PLA, broad-spectrum antibacterial) and 2-phenylethanol (2-PE, a pleasant rose-like fragrance) biosynthesis pathway were co-constructed in the host with circuit to achieve antibacterial effect and natural deodorizing effect.

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Safe project design

Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante. When we first started thinking about our project, we had grand plans to carry out our initial experiments in E. coli before completing proof-of-concept in the ESKAPE pathogens themselves. We soon realised that working with these pathogens was unwise from a safety standpoint, and was not permitted by either iGEM and University of Edinburgh safety guidelines, so we altered our plan and developed new experiments so that all proof-of-concept work could be carried out in non-pathogenic E. coli. We liaised with the University of Edinburgh Biological Safety Committee to create a safe system that would allow us to achieve our goals. Our first plan for the E. coli system was to use promoter-less resistance genes. Through discussions with the safety committee we realised that the risks of expression due to cryptic promoters or spread through horizontal gene transfer were not insignificant. We altered the design of our system to ensure that no functional resistance gene products would be expressed by using short “chunks” of the gene, rather than the full sequence.

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Safe shipment

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