Difference between revisions of "Team:ZJU-China"

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{{ZJU-China}}
 
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <h1> What we do</h1>
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    <p>This year, we are developing a project which utilizes three orthogonal connection systems to detect enzyme-catalyzed reaction products qualitatively and quantitatively. </p>
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    <p>Although enzymes can be used in purified form, in crude cell lysates, or encased in synthetic protective materials, an approach that optimally combines the criteria of high surface area, enhanced enzyme stability, rapid mass transport, and modularity remains elusive.  Recently, a brand-new protein immobilization platform has been explored. The platform modifies curli nanofibers, the amyloid fiber component of E. coli biofilms, with a peptide tag fused to the amyloidogenic protein CsgA.</p>
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    <p>Meanwhile, a genetically programmable, irreversible immobilization method—the spontaneous covalent bond formation between 13-amino acid SpyTag and 15kDa SpyCatcher has been demonstrated.  CsgA with a fused SpyTag and a certain enzyme with a SpyCatcher fusion are proved to form site-specific attachment between tags and catchers, even in a complex mixture. </p>
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    <p>In our approach, we are going to transform PHL628-△csgA cells with pBbE1a plasmids expressing CsgA-SpyTag to cover cells with curli fibers as the base. Curli fibers will connect adjacent cells to form a biofilm(Fig.1). Then, since there are three orthogonal systems, SpyTag/SpyCatcher2, SnoopTag/SnoopCatcher  and SdyTag/SdyCatcher , we are also going to synthesizing a string of three enzymes rather than one single SpyCatcher-Enzyme. The method is specifically illustrated as follows(Fig.2).</p>
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    <h1> Where the value lies</h1>
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    <p>One advantage is that it promises to evolve into a cell-free system with high fidelity. It is reported the biofilm displaying the same level of activity after 12 days with a slight decrease after 28 days, which indicates that the biofilm and its entangled curli fibers are stable enough.Thus, a reasonable assumption is that even if cells die, curli with SpyTag and the string of enzymes still works. In view of this advantage, the assembled system has the potential to be widely practiced in industry.  </p>
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    <p>Another advantage is that the string has a myriad of multi-enzyme systems by assigning specific functions to each enzyme at different sites. For example, a logic network composed of three enzymes, alcohol dehydrogenase (ADH, from Saccharomyces cerevisiae), glucose dehydrogenase (GDH, from Pseudomonas) and glucose oxidase (GOx, from Aspergillus niger) operating in concert as four concatenated logic gates(AND/OR) was designed to process 4 different chemical input signals (NAD, acetaldehyde, glucose and oxygen) (Fig.3).  The conceived functions of the three enzymes will be discussed later. There is no exaggeration that microelements, such as domains and subunits, provide a possibility to achieve multiple functions.</p>
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  <img class="picture3" src="img/ZJUChina_figure3.png">
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  <h1> How hardware assists</h1>
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  <h2> Microfluidic devices</h2>
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    <p>It is acknowledged that culture of cells using various microfluidic devices is becoming more common within experimental cell biology. Based on mature technology of microfluidic cell culture, we attempt to control over experimental conditions precisely via custom designed chip architectures, parallelization, automation and direct coupling to miniaturized downstream analysis platforms. This is how purified products, the strings of three enzymes, are produced. </p>
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    <h2> 3D printing</h2>
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<h3>Before you start</h3>
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    <p>A method using ink-jet printing for constructing multi-enzyme systems was proposed. Considering the vaporization of liquids on ink-jet printers while printing, which may lead to protein denaturation and enzyme inactivation, we decided to transform a 3D printer with similar principles. The capability of 3D printer in creating a precise two-dimensional distribution of enzymes indicates a possibility of spatially controlling enzymatic reactions. </p>
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<p> Please read the following pages:</p>
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    <h2> Biochip</h2>
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    <p>As for the application of our model, it is diversified and remains infinite possibilities. We have already cited one application called enzyme-based logic systems above. Moreover, biochips are preferred. The original intention is to monitor enzyme-catalyzed reaction in real-time with sensitive currents which is generated by the enzymatic biofuel cells (EBFCs) and reflects the product concentration and other indicators. </p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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    <p>Adopting nanostructured materials for biofuel cell construction has been extensively suggested as an effective and promising strategy to achieve high energy production; nanotubes can directly connect the enzyme active centers and transport the produced electrons to the electrode along the tubular network. The bacteria growing on the nanotubes enables enzymes on the curli-connected string to close contact nanotubes, which acts as an anode. In addition, metal nanoparticles are similar in size to some enzymes, thus decreasing the electron transfer distance of the bioelectrodes. So we will interface curli fibers with gold nanoparticles(AuNPs) as well to further improve performance of EBFCs(Fig.4).  Laccases with electrodes yields the cathode units of EBFCs.</p>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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  <img class="picture4" src="img/ZJUChina_figure4.png">
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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        <div class="item active "><img src="img/ZJUChina_home_undergraduate.png" /></div>
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        <div class="item active "><img src="img/ZJUChina_home_biolab.png" /></div>
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        <div class="item active "><img src="img/ZJUChina_home_foundation.png" /></div>
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<p>Copyright © 2018 ZJU-IGEM | <a href="www.zju.edu.cn">Zhejiang University</a> |</p>
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  <li><img src="img/ZJUChina_home_biolab.png" alt="" width="200"></li>
 
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  <li><img src="img/ZJUChina_home_medicine.png" alt="" width="200"></li>
 
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  <li><img src="img/ZJUChina_home_cs.png" alt="" width="200"></li>
 
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  <li><img src="img/ZJUChina_home_foundation.png" alt="" width="200"></li>
 
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<p>Copyright © 2018 ZJU-IGEM | <a href="www.zju.edu.cn">Zhejiang University</a> |</p>
 
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<div class="column full_size" >
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 +
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
  
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<div class="clear extra_space"></div>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="button_link">
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<a href="https://2018.igem.org/Special:Upload">
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UPLOAD FILES
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</a>
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</div>
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<div class="column third_size" >
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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</div>
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<div class="column third_size" >
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<div class="highlight decoration_B_full">
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2018.igem.org/wiki/index.php?title=Team:ZJU-China&action=edit">
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EDIT PAGE
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</a>
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 +
<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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</div>
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<div class="column third_size">
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
 
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Revision as of 11:38, 10 June 2018

HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Notebook
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Results
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM ⇗

Welcome to iGEM 2018!

Your team has been approved and you are ready to start the iGEM season!

Before you start

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Uploading pictures and files

You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

UPLOAD FILES

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page

EDIT PAGE

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
  • Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples: