Difference between revisions of "Team:NU Kazakhstan/Notebook"

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{{NU_Kazakhstan}}
 
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<!-- Start Banner Area -->
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<section class="generic-banner relative" style="background: url(http://highline.codal.kz/img/img-header-overview.JPG); background-size: cover; ">
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<div class="col-lg-10">
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<div class="col-lg-12">
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<div class="banner-content text-center">
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<h1 style="color: #fff; border-bottom: none; font-weight: bold!important">Overview & Background</h1>
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</div>
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</div>
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</div>
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</div>
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</section>
  
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<section class="sample-text-area">
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<div class="container">
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<div class="container">
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<h3 class="text-heading"><span>PROTOCOLS</span> <a href="#"><span>PROJECT TIMELINE</span></a></h3>
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<section class="sample-text-area">
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<div class="container">
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<u><b>Subculture of Cyanobacteria</b></u>
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<ol>
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<li>1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media</li>
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<li>2. Place into fresh 50 mL BG11-media</li>
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<li>3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood</li>
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<li>4. Check OD at 750nm</li>
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<ol>
  
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<div class="column full_size">
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<u><b>Preparation of BG11-media</b></u>
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<b>Stock solutions for BG-11:<br>
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&nbsp;A) Stock 1:</b><br>
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<table>
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<tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr>
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tr><td>NFerric ammonium citrate</td><td>0.6g/liter</td></tr>
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tr><td>NCitric acid . 1H2O</td><td>0.6g/liter</td></tr>
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tr><td>NCaCl2 . 2H2O</td><td>3.6g/liter</td></tr>
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</table>
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Filter sterilize into a sterile bottle or autoclave
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</div>
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<h6 class="text-uppercase mb-20">Top Menu</h6>
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<ul class="footer-nav">
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan">Home</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Description">Project</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Team">Team</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Notebook">Notebook</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Human_Practices">Human Practices</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/InterLab">Interlab</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Composite_Part">Composite Part</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Safety">Safety</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Judging_form">Judging form</a></li>
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</ul>
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</div>
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<div class="col-lg-3 col-sm-6">
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<div class="single-footer-widget">
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<h6 class="text-uppercase mb-20">Project</h6>
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<ul class="footer-nav">
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Description">Overview & Description</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Design">Design</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Parts">Parts</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Modeling">Modeling</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Results">Results</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Future_Plans">Future Plans</a></li>
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</ul>
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</div>
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</div>
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<div class="col-lg-3 col-sm-6">
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<div class="single-footer-widget">
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<h6 class="text-uppercase mb-20">Judging form</h6>
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<ul class="footer-nav">
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Judging_form">Best Human Integrated Practices</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Entrepreneurship">Best Education Integrated Practices</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Best_Modeling">Best Modeling</a></li>
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<li><a href="https://2018.igem.org/Team:NU_Kazakhstan/Measurement">Best Plant Synthetic biology</a></li>
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</ul>
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</div>
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</div>
  
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<h1>Notebook</h1>
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<div class="col-lg-3 col-sm-6">
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<div class="single-footer-widget">
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<h6 class="text-uppercase mb-20">Quick About</h6>
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</div>
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<p>
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<div class="clear"></div>
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SCHOOL OF SCIENCE AND TECHNOLOGY Nazarbayev University Astana, Kazakhstan
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</p>
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<p>
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+7 701 221 8710 <br>
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<div class="column two_thirds_size">
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aidana.toleshova@nu.edu.kz
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<h3>What should this page have?</h3>
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</p>
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<ul>
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<div class="footer-social d-flex align-items-center">
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<li>Chronological notes of what your team is doing.</li>
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<a href="https://www.facebook.com/nukazakhstan/"><i class="fa fa-facebook"></i></a>
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<li> Brief descriptions of daily important events.</li>
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<a href="ttps://www.instagram.com/nu_kazakhstan.igem/"><i class="fa fa-instagram"></i></a>
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<li>Pictures of your progress. </li>
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</div>
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<li>Mention who participated in what task.</li>
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</div>
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</ul>
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</div>
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</div>
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</div>
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<div class="footer-bottom d-flex justify-content-between align-items-center flex-wrap">
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<!-- Link back to Colorlib can't be removed. Template is licensed under CC BY 3.0. -->
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<div class="column third_size">
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<p class="foter-text m-0">Copyright &copy;<script>document.write(new Date().getFullYear());</script> All rights reserved</p>
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<div class="highlight decoration_A_full">
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<!-- Link back to Colorlib can't be removed. Template is licensed under CC BY 3.0. -->
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<h3>Inspiration</h3>
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</div>
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<p>You can see what others teams have done to organize their notes:</p>
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</div>
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</footer>
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<ul>
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<!-- End Footer Area -->
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</ul>
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</div>
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</div>
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Revision as of 07:13, 12 October 2018

Bioremediation of Sour Crude Oil Waste using Cyanobacteria




PROTOCOLS PROJECT TIMELINE

Subculture of Cyanobacteria
  1. 1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
  2. 2. Place into fresh 50 mL BG11-media
  3. 3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
  4. 4. Check OD at 750nm
      5. Preparation of BG11-media Stock solutions for BG-11:
       A) Stock 1:
      tr> tr> tr>
      Na2MG EDTA0.1g/liter
      NFerric ammonium citrate0.6g/liter
      NCitric acid . 1H2O0.6g/liter
      NCaCl2 . 2H2O3.6g/liter
      Filter sterilize into a sterile bottle or autoclave