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Revision as of 07:18, 12 October 2018
PROTOCOLS PROJECT TIMELINE&nbps;
Subculture of Cyanobacteria
- 1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
- 2. Place into fresh 50 mL BG11-media
- 3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
- 4. Check OD at 750nm
-
Preparation of BG11-media
Stock solutions for BG-11:
A) Stock 1:
Na2MG EDTA | 0.1g/liter | NFerric ammonium citrate | 0.6g/liter | tr>NCitric acid . 1H2O | 0.6g/liter | tr>NCaCl2 . 2H2O | 3.6g/liter |