Difference between revisions of "Team:NU Kazakhstan/Notebook"
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| − | <h1 style="color: #fff; border-bottom: none; font-weight: bold!important"> | + | <h1 style="color: #fff; border-bottom: none; font-weight: bold!important">Overview & Background</h1> |
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| − | <h3 class="text-heading"><span>PROTOCOLS</span> <a href="#"><span style="border-bottom: 1px dashed #777; "> PROJECT TIMELINE</span></a></h3> | + | <h3 class="text-heading"><span>PROTOCOLS</span> <a href="#"><span style="border-bottom: 1px dashed #777; "> PROJECT TIMELINE &nbps;</span></a></h3> |
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<tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | <tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | ||
| − | tr><td>NFerric ammonium citrate</td><td>0.6g/liter</td></tr> | + | <tr><td>NFerric ammonium citrate</td><td>0.6g/liter</td></tr> |
| − | tr><td>NCitric acid . 1H2O</td><td>0.6g/liter</td></tr> | + | <tr><td>NCitric acid . 1H2O</td><td>0.6g/liter</td></tr> |
| − | tr><td>NCaCl2 . 2H2O</td><td>3.6g/liter</td></tr> | + | <tr><td>NCaCl2 . 2H2O</td><td>3.6g/liter</td></tr> |
</table> | </table> | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
Revision as of 07:19, 12 October 2018
PROTOCOLS PROJECT TIMELINE &nbps;
Subculture of Cyanobacteria
- 1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
- 2. Place into fresh 50 mL BG11-media
- 3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
- 4. Check OD at 750nm
-
Preparation of BG11-media
Stock solutions for BG-11:
A) Stock 1:
| Na2MG EDTA | 0.1g/liter |
| NFerric ammonium citrate | 0.6g/liter |
| NCitric acid . 1H2O | 0.6g/liter |
| NCaCl2 . 2H2O | 3.6g/liter |