Difference between revisions of "Team:NU Kazakhstan/Notebook"

Revision as of 07:31, 12 October 2018

Bioremediation of Sour Crude Oil Waste using Cyanobacteria

Subculture of Cyanobacteria

1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
2. Place into fresh 50 mL BG11-media
3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
4. Check OD at 750nm

Preparation of BG11-media Stock solutions for BG-11:
A) Stock 1:

Filter sterilize into a sterile bottle or autoclave
Stock 2:

MgSO4 . 7H2O

7.5g/liter

Filter sterilize into a sterile bottle or autoclave
Stock 3:

K2HPO4 . 3H2O	4.0g/liter
or K2HPO4	3.05g/liter

Filter sterilize into a sterile bottle or autoclave
Stock 5 (Microelements):

@@ Line 34: / Line 34: @@
 	<li>3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood</li>
 	<li>4. Check OD at 750nm</li>
-<ol>
+</ol>
 <u><b>Preparation of BG11-media</b></u>
@@ Line 46: / Line 46: @@
 </table>
 Filter sterilize into a sterile bottle or autoclave
+<br>
+&nbsp; Stock 2:</b><br>
+<table>
+<tr><td>MgSO4 . 7H2O</td><td>7.5g/liter</td></tr>
+</table>
+Filter sterilize into a sterile bottle or autoclave
+<br>
+&nbsp;Stock 3:</b><br>
+<table>
+<tr><td>K2HPO4 . 3H2O</td><td>4.0g/liter</td></tr>
+<tr><td>or K2HPO4</td><td>3.05g/liter</td></tr>
+</table>
+Filter sterilize into a sterile bottle or autoclave
+<br><b>Stock 5 (Microelements):</b></br>
+<table>
+<tr><td>H3BO3</td><td>2.86g/liter</td></tr>
+<tr><td>MnCl2 . 4H2O</td><td>1.81g/liter</td></tr>
+<tr><td>ZnSO4 . 7H2O</td><td>0.222g/liter</td></tr>
+<tr><td>CuSO4 . 5H2O</td><td>0.079g/liter</td></tr>
+<tr><td>COCl2 . 6H2O</td><td>0.050g/liter</td></tr>
+<tr><td>NaMoO4 . 2H2O</td><td>0.391g/liter</td></tr>
+<tr><td>or MoO4 (85%)</td><td>0.018g/liter</td></tr>
+</table>
 			</div>
 		</section>