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<h1>cDNA Conversion</h1> | <h1>cDNA Conversion</h1> | ||
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+ | <h2>What is cDNA synthesis and why is it needed?</h2> | ||
+ | <p> cDNA stands for complementary DNA, and can be described as a double stranded DNA that is made with RNA as template. Our ultimate goal is the sequence the RNA that we have extracted - and with our sequencing method of choice, we need to turn the RNA back into DNA for it to be read properly. To do this, we use a technique known as reverse transcription to read and copy the RNA contents onto a newly made DNA strand, with no genetic information lost! | ||
+ | </p> | ||
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+ | <h2>How is it done?</h2> | ||
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Revision as of 07:47, 14 October 2018
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cDNA Conversion
What is cDNA synthesis and why is it needed?
cDNA stands for complementary DNA, and can be described as a double stranded DNA that is made with RNA as template. Our ultimate goal is the sequence the RNA that we have extracted - and with our sequencing method of choice, we need to turn the RNA back into DNA for it to be read properly. To do this, we use a technique known as reverse transcription to read and copy the RNA contents onto a newly made DNA strand, with no genetic information lost!