Difference between revisions of "Team:Edinburgh UG/Experiments"
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<h1 class="brand-heading">General Protocols</h1> | <h1 class="brand-heading">General Protocols</h1> | ||
<h2 style="text-align:left">Agarose Gel</h2> | <h2 style="text-align:left">Agarose Gel</h2> | ||
| − | <ol><li> Weigh 1 g of agarose </li> <li>Add 100 mL of TAE 1X</li> <li>Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down” and put it back on the | + | <ol><li> Weigh 1 g of agarose </li> <li>Add 100 mL of TAE 1X</li> <li>Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down” |
| − | heat until the agarose is completely dissolved</li> <li>While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight</li> <li>When homogeneous, add 10 µL of SYBR | + | and put it back on the |
| + | heat until the agarose is completely dissolved</li> <li>While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight</li> <li>When | ||
| + | homogeneous, add 10 µL of SYBR | ||
SAFE DNA Gel Stain to the solution and mix well</li> <li>Pour the solution into the bed and clear all its bubbles with a pipette tip. Add in the comb and make sure it is secure</li> <li>Mix the samples with | SAFE DNA Gel Stain to the solution and mix well</li> <li>Pour the solution into the bed and clear all its bubbles with a pipette tip. Add in the comb and make sure it is secure</li> <li>Mix the samples with | ||
loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well</li> | loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well</li> | ||
| + | </ol> | ||
</div> | </div> | ||
Revision as of 18:53, 14 October 2018
Experiments
General Protocols
Agarose Gel
- Weigh 1 g of agarose
- Add 100 mL of TAE 1X
- Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down” and put it back on the heat until the agarose is completely dissolved
- While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight
- When homogeneous, add 10 µL of SYBR SAFE DNA Gel Stain to the solution and mix well
- Pour the solution into the bed and clear all its bubbles with a pipette tip. Add in the comb and make sure it is secure
- Mix the samples with loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well
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