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                   <li style="text-align:left">Mix the samples with loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well</li>
 
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            <h2 style="text-align:left">LB Medium (1 litre liquid)</h2>
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              <ol><li style="text-align:left"> 10 g tryptone</li>
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                  <li style="text-align:left">10 g NaCl</li>
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                  <li style="text-align:left">5 g yeast extract</li>
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                  <li style="text-align:left">Water</li>
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Revision as of 19:05, 14 October 2018

Edinburgh iGEM 2018

Experiments

General Protocols

Agarose Gel

  1. Weigh 1 g of agarose
  2. Add 100 mL of TAE 1X
  3. Heat up until the solution is homogeneous, avoiding boiling. If it boils, move away from the heat until it “calms down” and put it back on the heat until the agarose is completely dissolved
  4. While heating, prepare the bed in which the gel will polymerize. Make sure that it is well balanced and tight
  5. When homogeneous, add 10 µL of SYBR SAFE DNA Gel Stain to the solution and mix well
  6. Pour the solution into the bed and clear all its bubbles with a pipette tip. Add in the comb and make sure it is secure
  7. Mix the samples with loading dye in a 5:1 ratio. Put the samples into the wells, as well as 5 µL of DNA ladder into the first well

LB Medium (1 litre liquid)

  1. 10 g tryptone
  2. 10 g NaCl
  3. 5 g yeast extract
  4. Water

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