Difference between revisions of "Team:FJNU-China/Result"

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   <p>In the case of garbage lid, the lid should express the appropriate amount PLA&2-PE to sterilization and release fragrance.</p>
 
   <p>In the case of garbage lid, the lid should express the appropriate amount PLA&2-PE to sterilization and release fragrance.</p>
 
<p>
 
<p>
<h3 style="color: tomato; font-weight:bold;text-align:center;font-size:25px;">PLA</h3>
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<h3 style="color: tomato; font-weight:bold;text-align:center;font-size:25px;">2-PE</h3>
 
Goal:</br>
 
Goal:</br>
We used constitutive promoter, linked with three enzymes to express PLA. In order to make system in lid controllable, we need to figure out that how long and how much will PLA be produced. Then, go further and deduce how long the lid will start to work.</br>
+
Because we use phenylalanine as the substrate to biosynthesis 2-PE, it’s essential to know how much substrate should be put in our lid and how long 2-PE will release into the air. </br>
 
Reaults:</br>
 
Reaults:</br>
1. Relationship between time and PLA production.</br>
+
1. Relationship between substrate depletion and 2-PE production is shown. </br>
2. Relationship between cell concentration and PLA production.</br>
+
2. Further optimization.</br>
 
</p>
 
</p>
 
</div>
 
</div>
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                                 <a data-toggle="collapse" href="#collapse3"
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                                     Click here to know more about experimental details <span>[Expand]</span>  <i class="fa fa-hand-o-left" aria-hidden="true"></i>
 
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                                   <div class="col-md-12 col-xs-12">
   <p>&nbsp;&nbsp;&nbsp;&nbsp;1. Relationship between time and PLA production.</p>
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   <p>&nbsp;&nbsp;&nbsp;&nbsp; Relationship between substrate depletion and 2-PE production is shown.</p>
 
<div>
 
<div>
  <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/0/08/T--FJNU-China--D-PLA_production_and_time.png" style="width:60%"  >
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  <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/3/3d/T--FJNU-China--2-PE_subs_depletion_and_production.jpg" style="width:60%"  >
<p style="font-size:15px; text-align:center;">Figure 1.The line of time and PLA peoduction</p>
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<p style="font-size:15px; text-align:center;">Figure xxxx</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;We draw the line of time and different optical density (OD) of bacteria’s PLA production and found the accumulation of PLA reached 18mM at 5min, which means that the accumulated process of PLA is quite fast. According to the graphs, we can infer the best effecting time is 8 min for 15OD, 6.5 min for 22.5OD and 5 min for 30OD.</p>
+
<p>&nbsp;&nbsp;&nbsp;&nbspBy controlling other variables, the line between substrate depletion and 2-PE production is shown. According to our human practice, the most comfortable concentration sets at 5 mM.(more details on our human practice) We try to control the amount of substrate at 32.5 mM. </p>
 
</div>
 
</div>
  
   <p>&nbsp;&nbsp;&nbsp;&nbsp;2.Relationship between cell concentration and PLA production.</p>
+
   <p>&nbsp;&nbsp;&nbsp;&nbsp;2. Further optimization.</p>
 
<div>
 
<div>
 
  <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/1/12/T--FJNU-China--PLA_OD_and_production.jpg" style="width:60%"  >
 
  <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/1/12/T--FJNU-China--PLA_OD_and_production.jpg" style="width:60%"  >
 
<p style="font-size:15px; text-align:center;">Figure 2.The line of cell concentration and PLA production</p>
 
<p style="font-size:15px; text-align:center;">Figure 2.The line of cell concentration and PLA production</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;At the time we explored the mechanism of PLA sterilization, we found PLA inhibits the growth of harmful bacteria by break down the cell walls and membrane. It’s necessary to clarify the relationship between cell concentration and PLA production. The graph shows that the thicker engineered bacteria we use, the less PLA expressed instead. </br>
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the substrate of PLA is the same as 2-PE. To produce both of them, we have to add excess substrate. We came up with another idea, changing the RBS to increase the production of 2-PE. After we changed it to a strong RBS, the production of 2-PE increased to 5mM, which is suitable to put in the garbage lid.More details on our<a href="https://2018.igem.org/Team:FJNU-China/Model"> model</a> page.
&nbsp;&nbsp;&nbsp;&nbsp;This is also a join point of our model, more details on our<a href="https://2018.igem.org/Team:FJNU-China/Model"> model</a> page.
+
 
</p>
 
</p>
 
</div>
 
</div>

Revision as of 19:19, 15 October 2018

Result

  • Abstract
  • PLA
  • 2-PE
  • Garbage can
  • Bromidrosis
  • Biosafty
  • Demonstrate

PLA

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

 

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

PLA

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

 

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

Garbage lid

In the case of garbage lid, the lid should express the appropriate amount PLA&2-PE to sterilization and release fragrance.

PLA

Goal:
We used constitutive promoter, linked with three enzymes to express PLA. In order to make system in lid controllable, we need to figure out that how long and how much will PLA be produced. Then, go further and deduce how long the lid will start to work.
Reaults:
1. Relationship between time and PLA production.
2. Relationship between cell concentration and PLA production.

    1. Relationship between time and PLA production.

Figure 1.The line of time and PLA peoduction

    We draw the line of time and different optical density (OD) of bacteria’s PLA production and found the accumulation of PLA reached 18mM at 5min, which means that the accumulated process of PLA is quite fast. According to the graphs, we can infer the best effecting time is 8 min for 15OD, 6.5 min for 22.5OD and 5 min for 30OD.

    2.Relationship between cell concentration and PLA production.

Figure 2.The line of cell concentration and PLA production

    At the time we explored the mechanism of PLA sterilization, we found PLA inhibits the growth of harmful bacteria by break down the cell walls and membrane. It’s necessary to clarify the relationship between cell concentration and PLA production. The graph shows that the thicker engineered bacteria we use, the less PLA expressed instead.
    This is also a join point of our model, more details on our model page.

In the case of garbage lid, the lid should express the appropriate amount PLA&2-PE to sterilization and release fragrance.

2-PE

Goal:
Because we use phenylalanine as the substrate to biosynthesis 2-PE, it’s essential to know how much substrate should be put in our lid and how long 2-PE will release into the air.
Reaults:
1. Relationship between substrate depletion and 2-PE production is shown.
2. Further optimization.

     Relationship between substrate depletion and 2-PE production is shown.

Figure xxxx

   &nbspBy controlling other variables, the line between substrate depletion and 2-PE production is shown. According to our human practice, the most comfortable concentration sets at 5 mM.(more details on our human practice) We try to control the amount of substrate at 32.5 mM.

    2. Further optimization.

Figure 2.The line of cell concentration and PLA production

    However, the substrate of PLA is the same as 2-PE. To produce both of them, we have to add excess substrate. We came up with another idea, changing the RBS to increase the production of 2-PE. After we changed it to a strong RBS, the production of 2-PE increased to 5mM, which is suitable to put in the garbage lid.More details on our model page.

2-PE

Goal:
Because we use phenylalanine as the substrate to biosynthesis 2-PE, it’s essential to know how much substrate should be put in our lid and how long 2-PE will release into the air.
Reaults:
1. Relationship between substrate depletion and 2-PE production is shown.
2. Further optimization.

    1.Relationship between substrate depletion and 2-PE production is shown.

Figure 2.XXXXXXXXXXXX

    By controlling other variables, the line between substrate depletion and 2-PE production is shown. According to our human practice, the most comfortable concentration sets at 5 mM.(more details on our human practice) We try to control the amount of substrate at 32.5 mM.

    2. Further optimization.

    However, the substrate of PLA is the same as 2-PE. To produce both of them, we have to add excess substrate. We came up with another idea, changing the RBS to increase the production of 2-PE. After we changed it to a strong RBS, the production of 2-PE increased to 5mM, which is suitable to put in the garbage lid.

Bromidrosis

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

 

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

Bromidrosis

2-PE

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

 

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

Demonstrate

EurAsian Meetup

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

5th CCiC

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.