Difference between revisions of "Team:JMU Wuerzburg/Description"
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| + | <h2>Development of a qPCR-based rapid testen system</h2> | ||
| + | <p>Recently, fast qPCR and RTqPCR diagnostical tests detecting pathogens in patient-derived samples have been developed. These tests comprise all required steps from genome purification to the result analyses in a "single tube" reaction. Our aim is to construct a test system that is adaptable on various pathogens. At first we design primers and probes by analysing all published sequences of the respective pathogens with bioinformatic tools. After that we develop our rapid tests detecting Noroviruses and the malaria-causing pathogens Plasmodium. Our test system is evaluated and optimised using synthesised templates. In a second step, we use inactivated patient-derived samples to confirm selectivity and specificity of our tests. | ||
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<h1>Description</h1> | <h1>Description</h1> | ||
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p> | <p>Tell us about your project, describe what moves you and why this is something important for your team.</p> | ||
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<h3>What should this page contain?</h3> | <h3>What should this page contain?</h3> | ||
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<h3>Inspiration</h3> | <h3>Inspiration</h3> | ||
<p>See how other teams have described and presented their projects: </p> | <p>See how other teams have described and presented their projects: </p> | ||
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li> | <li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li> | ||
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<h3>Advice on writing your Project Description</h3> | <h3>Advice on writing your Project Description</h3> | ||
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. | We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. | ||
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<h3>References</h3> | <h3>References</h3> | ||
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p> | <p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p> | ||
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Revision as of 12:52, 26 June 2018
Development of a qPCR-based rapid testen system
Recently, fast qPCR and RTqPCR diagnostical tests detecting pathogens in patient-derived samples have been developed. These tests comprise all required steps from genome purification to the result analyses in a "single tube" reaction. Our aim is to construct a test system that is adaptable on various pathogens. At first we design primers and probes by analysing all published sequences of the respective pathogens with bioinformatic tools. After that we develop our rapid tests detecting Noroviruses and the malaria-causing pathogens Plasmodium. Our test system is evaluated and optimised using synthesised templates. In a second step, we use inactivated patient-derived samples to confirm selectivity and specificity of our tests.