Difference between revisions of "Team:DTU-Denmark/Parts"

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<p>This BioBrick is the coding region of MelA that has been codon-optimized to Aspergillus oryzae by DTU Biobuilders 2018. The protein sequence is based on a previous BioBrick.</p>
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<p>This BioBrick contains the coding region of<i> melA</i> codon optimized for <i>Aspergillus oryzae</i> by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.</p>
 
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<p>This BioBrick is the coding region for the Nat1 gene, that provides resistance to NCT in filamentous fungi.</p>
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<p>This BioBrick contains the coding region of the <i>nat1 gene</i> encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin. The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in <i>Aspergillus oryzae</i>.</p>
 
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Revision as of 10:16, 16 October 2018

Parts

Type

Coding

Length

1830

This BioBrick contains the coding region of melA codon optimized for Aspergillus oryzae by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.

Type

Coding

Length

573

This BioBrick contains the coding region of the nat1 gene encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin. The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in Aspergillus oryzae.

Type

Composite

Length

1290

This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1623

This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1626

This BioBrick encompasses the resistence gene Hph for expression in fungi. It has the promoter pTrpC and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2430

This BioBrick encompasses the MelA gene for expression in fungi. It has the promoter pTrpC and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2451

This BioBrick encompasses the MelA gene for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2924

This BioBrick contains the Hph selection marker and the chromoprotein AmilCP for expression in fungi.

Type

Composite

Length

3257

This BioBrick contains the Hph selection marker and the chromoprotein AmilCP for expression in fungi.