Difference between revisions of "Team:Uppsala/Protocol"

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<p>Throughout the worm buster project we have at many times felt the need for assistance or input on different parts of our project. Without those small but immensely meaningful things our project would never had gotten this far and in the worse case never been possible at all. Therefore we would like to express our gratitude to all the people that supported us throughout the project. </p>  
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<h1>Worm recovery from cups</h1>
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<p>Materials:<br>
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<ul>
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  <li>Cups</li>
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  <li>Faeces</li>
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  <li>Physiological solution (0.9% NaCl)</li>
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  <li>Tap water</li> </p>
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</u>
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<h2>Procedure</h2>
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<h3>Incubation of faeces</h3>
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<ul>
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  <li>Around 20 g of faeces are added to a cup</li>
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  <li>The faeces are stirred with tweezers to make the sample less compact</li>
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  <li>The cup is incubated at 28 °C for 1 week </li>
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</ul>
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<h3>Worm recovery</h3>
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<ul>
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  <li>The cup is filled with water (200 mL)</li>
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  <li>One half of a petri dish is placed on top. The cup with the petri dish is then turned upside down.</li>
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  <li>The cup is left at room temperature ON, to allow the dry material to soak water and deposit on the bottom.</li>
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  <li>The following day the cup with the petri dish are tilted to recover the liquid, which contains the worms</li>
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  <li>The worm solution is left in the fridge for 3 hours, to allow a complete deposit of the worms.</li>
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  <li>Most of the supernatant is removed, and the worm solution can be stored.</li>
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</p>
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</ul>
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<h1>Project Support and Advice</h1>
 
<h1>Project Support and Advice</h1>

Revision as of 13:55, 16 October 2018